J 2019

MicroRNA-210 expression during childbirth and postpartum as a potential biomarker of acute fetal hypoxia

VONKOVÁ, Barbara, Ivona BLAHÁKOVÁ, Lukáš HRUBAN, Petr JANKŮ, Šárka POSPÍŠILOVÁ et. al.

Basic information

Original name

MicroRNA-210 expression during childbirth and postpartum as a potential biomarker of acute fetal hypoxia

Authors

VONKOVÁ, Barbara (203 Czech Republic, belonging to the institution), Ivona BLAHÁKOVÁ (203 Czech Republic, belonging to the institution), Lukáš HRUBAN (203 Czech Republic), Petr JANKŮ (203 Czech Republic) and Šárka POSPÍŠILOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Biomedical Papers, Olomouc, Univerzita Palackého v Olomouci, 2019, 1213-8118

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30400 3.4 Medical biotechnology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 1.000

RIV identification code

RIV/00216224:14740/19:00108369

Organization unit

Central European Institute of Technology

DOI

UT WoS

000489991000011

Keywords in English

microRNA-210; acute fetal hypoxia; postpartum; clearance of miR-210

Tags

Tags

International impact, Reviewed
Změněno: 4/3/2020 13:23, Mgr. Pavla Foltynová, Ph.D.

Abstract

V originále

Objective. To explore whether miR-210 expression can be used as a diagnostic and prognostic marker in acute fetal hypoxia. Methods. Whole blood samples of 29 women and their fetuses without hypoxia and 24 women and their fetuses with hypoxia were analysed in this study. Reverse transcription and quantitative real-time PCR were used to measure the expression of miR-210. Expression level differences between the control and hypoxic group in labour time and postpartum change fold were analyzed by standard statistical tests. Results. We confirmed that miR-210 is significantly more upregulated in fetal blood with acute hypoxia when compared to maternal blood (P<0.001). Furthermore, there was significant up-regulation in miR-210 level in the hypoxic group when compared to the control non-hypoxic group (P<0.05) in both maternal and fetal blood. Our results did not confirm a significant difference in postpartum miR-210 clearance level 2 h, 8 h, 24 h or 48 h after labour. Conclusions. Our study confirmed miR-210 upregulation in the blood of pregnant women with acute fetal hypoxia at the time of labour compared to pregnant women without acute fetal hypoxia. Additional investigation should be done to determine miR-210 clearance and the possibility of using miR-210 as a diagnostic and prognostic marker.

Links

LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
MUNI/A/0968/2017, interní kód MU
Name: Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit V (Acronym: VýDiTeHeMA V)
Investor: Masaryk University, Category A
TE02000058, research and development project
Name: Centrum kompetence pro molekulární diagnostiku a personalizovanou medicínu (Acronym: MOLDIMED)
Investor: Technology Agency of the Czech Republic