MicroRNA-210 expression during childbirth and postpartum as a potential biomarker of acute fetal hypoxia

VONKOVÁ, Barbara, Ivona BLAHÁKOVÁ, Lukáš HRUBAN, Petr JANKŮ and Šárka POSPÍŠILOVÁ. MicroRNA-210 expression during childbirth and postpartum as a potential biomarker of acute fetal hypoxia. Biomedical Papers. Olomouc: Univerzita Palackého v Olomouci, 2019, vol. 163, No 3, p. 259-264. ISSN 1213-8118. Available from: https://dx.doi.org/10.5507/bp.2018.075.

Basic information

• Original name: MicroRNA-210 expression during childbirth and postpartum as a potential biomarker of acute fetal hypoxia
• Authors: VONKOVÁ, Barbara (203 Czech Republic, belonging to the institution), Ivona BLAHÁKOVÁ (203 Czech Republic, belonging to the institution), Lukáš HRUBAN (203 Czech Republic), Petr JANKŮ (203 Czech Republic) and Šárka POSPÍŠILOVÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Biomedical Papers, Olomouc, Univerzita Palackého v Olomouci, 2019, 1213-8118.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30400 3.4 Medical biotechnology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: https://biomed.papers.upol.cz/artkey/bio-201903-0011_microrna-210-expression-during-childbirth-and-postpartum-as-a-potential-biomarker-of-acute-fetal-hypoxia.php
• Impact factor: Impact factor: 1.000
• RIV identification code: RIV/00216224:14740/19:00108369
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.5507/bp.2018.075
• UT WoS: 000489991000011
• Keywords in English: microRNA-210; acute fetal hypoxia; postpartum; clearance of miR-210
• Tags: 14110212, podil, rivok
• Tags: International impact, Reviewed

Abstract

Objective. To explore whether miR-210 expression can be used as a diagnostic and prognostic marker in acute fetal hypoxia. Methods. Whole blood samples of 29 women and their fetuses without hypoxia and 24 women and their fetuses with hypoxia were analysed in this study. Reverse transcription and quantitative real-time PCR were used to measure the expression of miR-210. Expression level differences between the control and hypoxic group in labour time and postpartum change fold were analyzed by standard statistical tests. Results. We confirmed that miR-210 is significantly more upregulated in fetal blood with acute hypoxia when compared to maternal blood (P<0.001). Furthermore, there was significant up-regulation in miR-210 level in the hypoxic group when compared to the control non-hypoxic group (P<0.05) in both maternal and fetal blood. Our results did not confirm a significant difference in postpartum miR-210 clearance level 2 h, 8 h, 24 h or 48 h after labour. Conclusions. Our study confirmed miR-210 upregulation in the blood of pregnant women with acute fetal hypoxia at the time of labour compared to pregnant women without acute fetal hypoxia. Additional investigation should be done to determine miR-210 clearance and the possibility of using miR-210 as a diagnostic and prognostic marker.

Links

• LQ1601, research and development project: Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

• MUNI/A/0968/2017, interní kód MU: Name: Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit V (Acronym: VýDiTeHeMA V)

Investor: Masaryk University, Category A

• TE02000058, research and development project: Name: Centrum kompetence pro molekulární diagnostiku a personalizovanou medicínu (Acronym: MOLDIMED)

Investor: Technology Agency of the Czech Republic