Interleukin-6 contributes to initiation of neuronal
regeneration program in the remote dorsal root ganglia neurons
after sciatic nerve injury

J 2019

Interleukin-6 contributes to initiation of neuronal regeneration program in the remote dorsal root ganglia neurons after sciatic nerve injury

DUBOVÝ, Petr, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ, Václav BRÁZDA, Marek JOUKAL et. al.

Basic information

Original name

Interleukin-6 contributes to initiation of neuronal regeneration program in the remote dorsal root ganglia neurons after sciatic nerve injury

Authors

DUBOVÝ, Petr (203 Czech Republic, belonging to the institution), Ivana HRADILOVÁ SVÍŽENSKÁ (203 Czech Republic, belonging to the institution), Ilona KLUSÁKOVÁ (203 Czech Republic, belonging to the institution), Václav BRÁZDA (203 Czech Republic, belonging to the institution) and Marek JOUKAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Histochemistry and Cell Biology, Heidelberg, Springer, 2019, 0948-6143

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.418

RIV identification code

RIV/00216224:14110/19:00107745

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s00418-019-01779-3

UT WoS

000478661300003

Keywords in English

Primary sensory neuron; Sciatic nerve lesion; Ulnar nerve crush; SCG10; STAT3

Abstract

V originále

To assess the potential role of IL-6 in sciatic nerve injury-induced activation of a pro-regenerative state in remote dorsal root ganglia (DRG) neurons, we compared protein levels of SCG-10 and activated STAT3, as well as axon regeneration in IL-6 knockout (IL-6ko) mice and their wild-type (WT) counterparts. Unilateral sciatic nerve compression and transection upregulated SCG-10 protein levels and activated STAT3 in DRG neurons not only in lumbar but also in cervical segments of WT mice. A pro-regenerative state induced by prior sciatic nerve lesion in cervical DRG neurons of WT mice was also shown by testing for axon regeneration in crushed ulnar nerve. DRG neurons from IL-6ko mice also displayed bilaterally increased levels of SCG-10 and STAT3 in both lumbar and cervical segments after sciatic nerve lesions. However, levels of SCG-10 protein in lumbar and cervical DRG of IL-6ko mice were significantly lower than those of their WT counterparts. Sciatic nerve injury induced a lower level of SCG-10 in cervical DRG of IL-6ko than WT mice, and this correlates with significantly shorter regeneration of axons distal to the crushed ulnar nerve. These results suggest that IL-6 contributes, at the very least, to initiation of the neuronal regeneration program in remote DRG neurons after unilateral sciatic nerve injury.

Links

GA16-08508S, research and development project

Name: Zvýšení endogenního regeneračního programu a jeho aktivace zprostředkovaná cytokiny/chemokiny v neuronech ganglií bez spojení s poškozeným nervem (Acronym: ERP)

Investor: Czech Science Foundation

Citovat

DUBOVÝ, Petr, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ, Václav BRÁZDA and Marek JOUKAL. Interleukin-6 contributes to initiation of neuronal regeneration program in the remote dorsal root ganglia neurons after sciatic nerve injury. Histochemistry and Cell Biology. Heidelberg: Springer, 2019, vol. 152, No 2, p. 109-117. ISSN 0948-6143. Available from: https://dx.doi.org/10.1007/s00418-019-01779-3.

@article{1574658,
   author = {Dubový, Petr and Hradilová Svíženská, Ivana and Klusáková, Ilona and Brázda, Václav and Joukal, Marek},
   article_location = {Heidelberg},
   article_number = {2},
   doi = {http://dx.doi.org/10.1007/s00418-019-01779-3},
   keywords = {Primary sensory neuron; Sciatic nerve lesion; Ulnar nerve crush; SCG10; STAT3},
   language = {eng},
   issn = {0948-6143},
   journal = {Histochemistry and Cell Biology},
   title = {Interleukin-6 contributes to initiation of neuronal regeneration program in the remote dorsal root ganglia neurons after sciatic nerve injury},
   url = {http://dx.doi.org/10.1007/s00418-019-01779-3},
   volume = {152},
   year = {2019}
}

TY  - JOUR
ID  - 1574658
AU  - Dubový, Petr - Hradilová Svíženská, Ivana - Klusáková, Ilona - Brázda, Václav - Joukal, Marek
PY  - 2019
TI  - Interleukin-6 contributes to initiation of neuronal regeneration program in the remote dorsal root ganglia neurons after sciatic nerve injury
JF  - Histochemistry and Cell Biology
VL  - 152
IS  - 2
SP  - 109-117
EP  - 109-117
PB  - Springer
SN  - 09486143
KW  - Primary sensory neuron
KW  - Sciatic nerve lesion
KW  - Ulnar nerve crush
KW  - SCG10
KW  - STAT3
UR  - http://dx.doi.org/10.1007/s00418-019-01779-3
L2  - http://dx.doi.org/10.1007/s00418-019-01779-3
N2  - To assess the potential role of IL-6 in sciatic nerve injury-induced activation of a pro-regenerative state in remote dorsal root ganglia (DRG) neurons, we compared protein levels of SCG-10 and activated STAT3, as well as axon regeneration in IL-6 knockout (IL-6ko) mice and their wild-type (WT) counterparts. Unilateral sciatic nerve compression and transection upregulated SCG-10 protein levels and activated STAT3 in DRG neurons not only in lumbar but also in cervical segments of WT mice. A pro-regenerative state induced by prior sciatic nerve lesion in cervical DRG neurons of WT mice was also shown by testing for axon regeneration in crushed ulnar nerve. DRG neurons from IL-6ko mice also displayed bilaterally increased levels of SCG-10 and STAT3 in both lumbar and cervical segments after sciatic nerve lesions. However, levels of SCG-10 protein in lumbar and cervical DRG of IL-6ko mice were significantly lower than those of their WT counterparts. Sciatic nerve injury induced a lower level of SCG-10 in cervical DRG of IL-6ko than WT mice, and this correlates with significantly shorter regeneration of axons distal to the crushed ulnar nerve. These results suggest that IL-6 contributes, at the very least, to initiation of the neuronal regeneration program in remote DRG neurons after unilateral sciatic nerve injury.
ER  -

DUBOVÝ, Petr, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ, Václav BRÁZDA and Marek JOUKAL. Interleukin-6 contributes to initiation of neuronal regeneration program in the remote dorsal root ganglia neurons after sciatic nerve injury. \textit{Histochemistry and Cell Biology}. Heidelberg: Springer, 2019, vol.~152, No~2, p.~109-117. ISSN~0948-6143. Available from: https://dx.doi.org/10.1007/s00418-019-01779-3.

Detailed Information on Publication Record