Comparative Electrochemical and Spectroscopic Studies of
I-motif forming DNA Nonamers

J 2019

Comparative Electrochemical and Spectroscopic Studies of I-motif forming DNA Nonamers

TRNKOVÁ, Libuše, Iveta TŘÍSKOVÁ, Michaela VORLÍČKOVÁ, Iva KEJNOVSKÁ, Zuzana DVOŘÁKOVÁ et. al.

Basic information

Original name

Comparative Electrochemical and Spectroscopic Studies of I-motif forming DNA Nonamers

Authors

TRNKOVÁ, Libuše (203 Czech Republic, guarantor, belonging to the institution), Iveta TŘÍSKOVÁ (203 Czech Republic, belonging to the institution), Michaela VORLÍČKOVÁ (203 Czech Republic), Iva KEJNOVSKÁ (203 Czech Republic), Zuzana DVOŘÁKOVÁ (203 Czech Republic), Hana PIVOŇKOVÁ (203 Czech Republic) and Radovan FIALA (203 Czech Republic, belonging to the institution)

Edition

Electroanalysis, 2019, 1040-0397

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10400 1.4 Chemical sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

https://onlinelibrary.wiley.com/doi/epdf/10.1002/elan.201900323

Impact factor

Impact factor: 2.544

RIV identification code

RIV/00216224:14310/19:00111161

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/elan.201900323

UT WoS

000488393000001

Keywords in English

i-motif; (C)-rich DNA nonamers; linear sweep voltammetry; elimination voltammetry with linear scan; CD spectra; NMR spectra; native PAGE

Abstract

V originále

The paper shows the structural diversity of cytosine (C)-rich oligodeoxynucleotides (ODNs) arising from their detail nucleotide sequence and experimental conditions. In slightly acidic solutions, the ODN nonamers with different adenine (A) and cytosine (C) sequences can adopt non-canonical structures involving protonated bases. A distinct secondary structure formed in (C)-rich sequences, called i-motif (iM), consists of hemiprotonated and intercalated cytosine base pairs (C.C+). Folding and unfolding of particular structures in solutions were monitored by H-1 NMR and CD spectroscopies and native polyacrylamide gel electrophoresis (PAGE), which are capable to determine their structural characteristics. Effects of sequences and their proclivity to formation of the iM on electrochemical behaviour of the ODN nonamers were studied by electrochemical methods. The LSV signals of A and C obtained from the reductive dissolution of ODN adsorption layers on a hanging mercury drop electrode were processed by elimination voltammetry with linear scan (EVLS), which revealed complex effects of the nonamer properties (namely their primary and secondary structure confirmed in solution) on their adsorption and reduction activity.

Links

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/1359/2018, interní kód MU

Name: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2019

Investor: Masaryk University, Category A

90043, large research infrastructures

Name: CIISB

Citovat

TRNKOVÁ, Libuše, Iveta TŘÍSKOVÁ, Michaela VORLÍČKOVÁ, Iva KEJNOVSKÁ, Zuzana DVOŘÁKOVÁ, Hana PIVOŇKOVÁ and Radovan FIALA. Comparative Electrochemical and Spectroscopic Studies of I-motif forming DNA Nonamers. Electroanalysis. 2019, vol. 31, No 10, p. 2081-2093. ISSN 1040-0397. Available from: https://dx.doi.org/10.1002/elan.201900323.

@article{1576081,
   author = {Trnková, Libuše and Třísková, Iveta and Vorlíčková, Michaela and Kejnovská, Iva and Dvořáková, Zuzana and Pivoňková, Hana and Fiala, Radovan},
   article_number = {10},
   doi = {http://dx.doi.org/10.1002/elan.201900323},
   keywords = {i-motif; (C)-rich DNA nonamers; linear sweep voltammetry; elimination voltammetry with linear scan; CD spectra; NMR spectra; native PAGE},
   language = {eng},
   issn = {1040-0397},
   journal = {Electroanalysis},
   title = {Comparative Electrochemical and Spectroscopic Studies of I-motif forming DNA Nonamers},
   url = {https://onlinelibrary.wiley.com/doi/epdf/10.1002/elan.201900323},
   volume = {31},
   year = {2019}
}

TY  - JOUR
ID  - 1576081
AU  - Trnková, Libuše - Třísková, Iveta - Vorlíčková, Michaela - Kejnovská, Iva - Dvořáková, Zuzana - Pivoňková, Hana - Fiala, Radovan
PY  - 2019
TI  - Comparative Electrochemical and Spectroscopic Studies of I-motif forming DNA Nonamers
JF  - Electroanalysis
VL  - 31
IS  - 10
SP  - 2081-2093
EP  - 2081-2093
SN  - 10400397
KW  - i-motif
KW  - (C)-rich DNA nonamers
KW  - linear sweep voltammetry
KW  - elimination voltammetry with linear scan
KW  - CD spectra
KW  - NMR spectra
KW  - native PAGE
UR  - https://onlinelibrary.wiley.com/doi/epdf/10.1002/elan.201900323
L2  - https://onlinelibrary.wiley.com/doi/epdf/10.1002/elan.201900323
N2  - The paper shows the structural diversity of cytosine (C)-rich oligodeoxynucleotides (ODNs) arising from their detail nucleotide sequence and experimental conditions. In slightly acidic solutions, the ODN nonamers with different adenine (A) and cytosine (C) sequences can adopt non-canonical structures involving protonated bases. A distinct secondary structure formed in (C)-rich sequences, called i-motif (iM), consists of hemiprotonated and intercalated cytosine base pairs (C.C+). Folding and unfolding of particular structures in solutions were monitored by H-1 NMR and CD spectroscopies and native polyacrylamide gel electrophoresis (PAGE), which are capable to determine their structural characteristics. Effects of sequences and their proclivity to formation of the iM on electrochemical behaviour of the ODN nonamers were studied by electrochemical methods. The LSV signals of A and C obtained from the reductive dissolution of ODN adsorption layers on a hanging mercury drop electrode were processed by elimination voltammetry with linear scan (EVLS), which revealed complex effects of the nonamer properties (namely their primary and secondary structure confirmed in solution) on their adsorption and reduction activity.
ER  -

TRNKOVÁ, Libuše, Iveta TŘÍSKOVÁ, Michaela VORLÍČKOVÁ, Iva KEJNOVSKÁ, Zuzana DVOŘÁKOVÁ, Hana PIVOŇKOVÁ and Radovan FIALA. Comparative Electrochemical and Spectroscopic Studies of I-motif forming DNA Nonamers. \textit{Electroanalysis}. 2019, vol.~31, No~10, p.~2081-2093. ISSN~1040-0397. Available from: https://dx.doi.org/10.1002/elan.201900323.

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