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@article{1576081,
author = {Trnková, Libuše and Třísková, Iveta and Vorlíčková, Michaela and Kejnovská, Iva and Dvořáková, Zuzana and Pivoňková, Hana and Fiala, Radovan},
article_number = {10},
doi = {http://dx.doi.org/10.1002/elan.201900323},
keywords = {i-motif; (C)-rich DNA nonamers; linear sweep voltammetry; elimination voltammetry with linear scan; CD spectra; NMR spectra; native PAGE},
language = {eng},
issn = {1040-0397},
journal = {Electroanalysis},
title = {Comparative Electrochemical and Spectroscopic Studies of I-motif forming DNA Nonamers},
url = {https://onlinelibrary.wiley.com/doi/epdf/10.1002/elan.201900323},
volume = {31},
year = {2019}
}
TY - JOUR
ID - 1576081
AU - Trnková, Libuše - Třísková, Iveta - Vorlíčková, Michaela - Kejnovská, Iva - Dvořáková, Zuzana - Pivoňková, Hana - Fiala, Radovan
PY - 2019
TI - Comparative Electrochemical and Spectroscopic Studies of I-motif forming DNA Nonamers
JF - Electroanalysis
VL - 31
IS - 10
SP - 2081-2093
EP - 2081-2093
SN - 10400397
KW - i-motif
KW - (C)-rich DNA nonamers
KW - linear sweep voltammetry
KW - elimination voltammetry with linear scan
KW - CD spectra
KW - NMR spectra
KW - native PAGE
UR - https://onlinelibrary.wiley.com/doi/epdf/10.1002/elan.201900323
L2 - https://onlinelibrary.wiley.com/doi/epdf/10.1002/elan.201900323
N2 - The paper shows the structural diversity of cytosine (C)-rich oligodeoxynucleotides (ODNs) arising from their detail nucleotide sequence and experimental conditions. In slightly acidic solutions, the ODN nonamers with different adenine (A) and cytosine (C) sequences can adopt non-canonical structures involving protonated bases. A distinct secondary structure formed in (C)-rich sequences, called i-motif (iM), consists of hemiprotonated and intercalated cytosine base pairs (C.C+). Folding and unfolding of particular structures in solutions were monitored by H-1 NMR and CD spectroscopies and native polyacrylamide gel electrophoresis (PAGE), which are capable to determine their structural characteristics. Effects of sequences and their proclivity to formation of the iM on electrochemical behaviour of the ODN nonamers were studied by electrochemical methods. The LSV signals of A and C obtained from the reductive dissolution of ODN adsorption layers on a hanging mercury drop electrode were processed by elimination voltammetry with linear scan (EVLS), which revealed complex effects of the nonamer properties (namely their primary and secondary structure confirmed in solution) on their adsorption and reduction activity.
ER -
TRNKOVÁ, Libuše, Iveta TŘÍSKOVÁ, Michaela VORLÍČKOVÁ, Iva KEJNOVSKÁ, Zuzana DVOŘÁKOVÁ, Hana PIVOŇKOVÁ and Radovan FIALA. Comparative Electrochemical and Spectroscopic Studies of I-motif forming DNA Nonamers. \textit{Electroanalysis}. 2019, vol.~31, No~10, p.~2081-2093. ISSN~1040-0397. Available from: https://dx.doi.org/10.1002/elan.201900323.
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