J 2019

Oct4-mediated reprogramming induces embryonic-like microRNA expression signatures in human fibroblasts

PEŠKOVÁ, Lucie, Kateřina AMRUZ ČERNÁ, Jan OPPELT, Marek MRÁZ, Tomáš BÁRTA et. al.

Basic information

Original name

Oct4-mediated reprogramming induces embryonic-like microRNA expression signatures in human fibroblasts

Authors

PEŠKOVÁ, Lucie (203 Czech Republic, belonging to the institution), Kateřina AMRUZ ČERNÁ (203 Czech Republic, belonging to the institution), Jan OPPELT (203 Czech Republic, belonging to the institution), Marek MRÁZ (203 Czech Republic, belonging to the institution) and Tomáš BÁRTA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Scientific reports, LONDON, NATURE PUBLISHING GROUP, 2019, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 3.998

RIV identification code

RIV/00216224:14110/19:00107787

Organization unit

Faculty of Medicine

UT WoS

000493439600057

Keywords in English

ADULT HUMAN FIBROBLASTS; PLURIPOTENT STEM-CELLS; HUMAN SOMATIC-CELLS; DIRECT CONVERSION; MOUSE FIBROBLASTS; TRANSDIFFERENTIATION; ACQUISITION; ACTIVATION; REPRESSION; PLASTICITY

Tags

International impact, Reviewed
Změněno: 24/10/2024 16:37, Ing. Marie Švancarová

Abstract

V originále

Oct4-mediated reprogramming has recently become a novel tool for the generation of various cell types from differentiated somatic cells. Although molecular mechanisms underlying this process are unknown, it is well documented that cells over-expressing Oct4 undergo transition from differentiated state into plastic state. This transition is associated with the acquisition of stem cells properties leading to epigenetically "open" state that is permissive to cell fate switch upon external stimuli. In order to contribute to our understanding of molecular mechanisms driving this process, we characterised human fibroblasts over-expressing Oct4 and performed comprehensive small-RNAseq analysis. Our analyses revealed new interesting aspects of Oct4-mediated cell plasticity induction. Cells over-expressing Oct4 lose their cell identity demonstrated by down-regulation of fibroblast-specific genes and up-regulation of epithelial genes. Interestingly, this process is associated with microRNA expression profile that is similar to microRNA profiles typically found in pluripotent stem cells. We also provide extensive network of microRNA families and clusters allowing us to precisely determine the miRNAome associated with the acquisition of Oct4-induced transient plastic state. Our data expands current knowledge of microRNA and their implications in cell fate alterations and contributing to understanding molecular mechanisms underlying it.

Links

GJ16-24004Y, research and development project
Name: Indukce buněčné plasticity prostřednictvím modulace mikroRNA molekul: Nový přístup pro přeprogramování buněk
Investor: Czech Science Foundation
LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
90091, large research infrastructures
Name: NCMG