JELÍNKOVÁ, Šárka, I KRATOCHVÍLOVÁ, Martin PEŠL, Jan PŘIBYL, Petr SKLÁDAL, Vladimír ROTREKL and Jan PŘIBYL. 5. Influence of selected cryoprotectants on regeneration of cryopreserved cells properties. Online. In Haider Khawaja. Stem Cells: From Myth to Reality and Evolving. 1st ed. Springer, 2019, p. 102-126, 24 pp.
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Basic information
Original name 5. Influence of selected cryoprotectants on regeneration of cryopreserved cells properties
Name (in English) 5. Influence of selected cryoprotectants on regeneration of cryopreserved cells properties
Authors JELÍNKOVÁ, Šárka, I KRATOCHVÍLOVÁ, Martin PEŠL, Jan PŘIBYL, Petr SKLÁDAL, Vladimír ROTREKL and Jan PŘIBYL.
Edition 1. vyd. Stem Cells: From Myth to Reality and Evolving, p. 102-126, 24 pp. 2019.
Publisher Springer
Other information
Type of outcome Chapter(s) of a specialized book
Confidentiality degree is not subject to a state or trade secret
Publication form electronic version available online
Keywords in English Cryopreservation, rCyoprotectants, Freeze, Multipotent, Pluripotent, Saccharose, Stem cells, Therapy, Trehalose.
Tags topvydavatel
Changed by Changed by: MUDr. Martin Pešl, Ph.D., učo 60014. Changed: 7/1/2020 22:33.
Abstract
The cryopreservation of cells is a fundamental task for a wide range of applications, from basic storage of cells to fertilization. Cells must be protected against damage during freezing by application of specifíc cryoprotectants and freezing/melting protocols. This chapter presents a brief overview of the predominant cryopreservation methods and most common cryoprotectants. Furthermore, it explores the specifics of cryopreservation of stem cells with various types and levels of multipotency. Special attention is dedicated to the effect of cryopreservation on human pluripotent stem cells, including the effect of different cryoprotectants used to prepare fibroblast feeder layer for the cultivation of pluripotent cells. Finally, the chapter concludes with a review of selected methods that have been successfully used to probe the postthaw state of cryopreserved cells through their viability, inner structure, and mechanical properties.
Abstract (in English)
The cryopreservation of cells is a fundamental task for a wide range of applications, from basic storage of cells to fertilization. Cells must be protected against damage during freezing by application of specifíc cryoprotectants and freezing/melting protocols. This chapter presents a brief overview of the predominant cryopreservation methods and most common cryoprotectants. Furthermore, it explores the specifics of cryopreservation of stem cells with various types and levels of multipotency. Special attention is dedicated to the effect of cryopreservation on human pluripotent stem cells, including the effect of different cryoprotectants used to prepare fibroblast feeder layer for the cultivation of pluripotent cells. Finally, the chapter concludes with a review of selected methods that have been successfully used to probe the postthaw state of cryopreserved cells through their viability, inner structure, and mechanical properties.
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