Detailed Information on Publication Record
2019
5. Influence of selected cryoprotectants on regeneration of cryopreserved cells properties
JELÍNKOVÁ, Šárka, I KRATOCHVÍLOVÁ, Martin PEŠL, Jan PŘIBYL, Petr SKLÁDAL et. al.Basic information
Original name
5. Influence of selected cryoprotectants on regeneration of cryopreserved cells properties
Name (in English)
5. Influence of selected cryoprotectants on regeneration of cryopreserved cells properties
Authors
JELÍNKOVÁ, Šárka, I KRATOCHVÍLOVÁ, Martin PEŠL, Jan PŘIBYL, Petr SKLÁDAL, Vladimír ROTREKL and Jan PŘIBYL
Edition
1. vyd. Stem Cells: From Myth to Reality and Evolving, p. 102-126, 24 pp. 2019
Publisher
Springer
Other information
Type of outcome
Kapitola resp. kapitoly v odborné knize
Confidentiality degree
není předmětem státního či obchodního tajemství
Publication form
electronic version available online
ISBN
978-3-11-064243-8
Keywords in English
Cryopreservation, Cryoprotectants, Freeze, Multipotent, Pluripotent, Saccharose, Stem cells, Therapy, Trehalose.
Tags
Změněno: 19/8/2024 11:09, MUDr. Martin Pešl, Ph.D.
V originále
The cryopreservation of cells is a fundamental task for a wide range of applications, from basic storage of cells to fertilization. Cells must be protected against damage during freezing by application of specifíc cryoprotectants and freezing/melting protocols. This chapter presents a brief overview of the predominant cryopreservation methods and most common cryoprotectants. Furthermore, it explores the specifics of cryopreservation of stem cells with various types and levels of multipotency. Special attention is dedicated to the effect of cryopreservation on human pluripotent stem cells, including the effect of different cryoprotectants used to prepare fibroblast feeder layer for the cultivation of pluripotent cells. Finally, the chapter concludes with a review of selected methods that have been successfully used to probe the postthaw state of cryopreserved cells through their viability, inner structure, and mechanical properties.
In English
The cryopreservation of cells is a fundamental task for a wide range of applications, from basic storage of cells to fertilization. Cells must be protected against damage during freezing by application of specifíc cryoprotectants and freezing/melting protocols. This chapter presents a brief overview of the predominant cryopreservation methods and most common cryoprotectants. Furthermore, it explores the specifics of cryopreservation of stem cells with various types and levels of multipotency. Special attention is dedicated to the effect of cryopreservation on human pluripotent stem cells, including the effect of different cryoprotectants used to prepare fibroblast feeder layer for the cultivation of pluripotent cells. Finally, the chapter concludes with a review of selected methods that have been successfully used to probe the postthaw state of cryopreserved cells through their viability, inner structure, and mechanical properties.