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@article{1582160,
author = {Kushkevych, Ivan and Kotrsová, Věra and Dordević, Dani and Buňková, Leona and Vítězová, Monika and Amedei, Amedeo},
article_number = {12},
doi = {http://dx.doi.org/10.3390/biom9120752},
keywords = {hydrogen sulfide; toxicity; intestinal microbiome; sulfate-reducing bacteria; lactic acid bacteria; inflammatory bowel disease; ulcerative colitis},
language = {eng},
issn = {2218-273X},
journal = {Biomolecules},
title = {Hydrogen Sulfide Effects on the Survival of Lactobacilli with Emphasis on the Development of Inflammatory Bowel Diseases},
url = {https://www.mdpi.com/2218-273X/9/12/752},
volume = {9},
year = {2019}
}
TY - JOUR
ID - 1582160
AU - Kushkevych, Ivan - Kotrsová, Věra - Dordević, Dani - Buňková, Leona - Vítězová, Monika - Amedei, Amedeo
PY - 2019
TI - Hydrogen Sulfide Effects on the Survival of Lactobacilli with Emphasis on the Development of Inflammatory Bowel Diseases
JF - Biomolecules
VL - 9
IS - 12
SP - 1-13
EP - 1-13
PB - MDPI
SN - 2218273X
KW - hydrogen sulfide
KW - toxicity
KW - intestinal microbiome
KW - sulfate-reducing bacteria
KW - lactic acid bacteria
KW - inflammatory bowel disease
KW - ulcerative colitis
UR - https://www.mdpi.com/2218-273X/9/12/752
L2 - https://www.mdpi.com/2218-273X/9/12/752
N2 - The gut microbiota is a complex component of humans that depends on diet, host genome, and lifestyle. The background: The study purpose is to find relations between nutrition, intestinal lactic acid bacteria (LAB) from various environments (human, animal intestine, and yogurt) and sulfate-reducing microbial communities in the large intestine; to compare kinetic growth parameters of LAB; and to determine their sensitivity to different concentration of hydrogen sulfide produced by intestinal sulfate-reducing bacteria. Methods: Microbiological (isolation and identification), biochemical (electrophoresis), molecular biology methods (DNA isolation and PCR analysis), and statistical processing (average and standard error calculations) of the results were used. The results: The toxicity of hydrogen sulfide produced by sulfate-reducing bacteria, the survival of lactic acid bacteria, and minimal inhibitory concentrations (MIC) were determined. The measured hydrogen sulfide sensitivity values were the same for L. paracasei and L. reuteri (MIC > 1.1 mM). In addition, L. plantarum and L. fermentum showed also a similar sensitivity (MIC > 0.45 mM) but significantly (p < 0.05) lower than L. reuteri and L. paracasei (1.1 > 0.45 mM). L. paracasei and L. reuteri are more sensitive to hydrogen sulfide than L. fermentum and L. plantarum. L. pentosus was sensitive to the extremely low concentration of H2S (MIC > 0.15 mM). Conclusions: The Lactobacillus species were significantly sensitive to hydrogen sulfide, which is a final metabolite of intestinal sulfate-reducing bacteria. The results are definitely helpful for a better understanding of complicated interaction among intestinal microbiota and nutrition.
ER -
KUSHKEVYCH, Ivan, Věra KOTRSOVÁ, Dani DORDEVI$\backslash$'C, Leona BUŇKOVÁ, Monika VÍTĚZOVÁ and Amedeo AMEDEI. Hydrogen Sulfide Effects on the Survival of Lactobacilli with Emphasis on the Development of Inflammatory Bowel Diseases. \textit{Biomolecules}. MDPI, 2019, vol.~9, No~12, p.~1-13. ISSN~2218-273X. Available from: https://dx.doi.org/10.3390/biom9120752.
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