One-step detection of human papilloma viral infection using
quantum dot-nucleotide interaction specificity

J 2019

One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity

JIMENEZ, Ana Maria Jimenez, Amitava MOULICK, Sukanya BHOWMICK, Vladislav STRMISKA, Milica GAGIC et. al.

Basic information

Original name

One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity

Authors

JIMENEZ, Ana Maria Jimenez (203 Czech Republic), Amitava MOULICK (203 Czech Republic), Sukanya BHOWMICK (203 Czech Republic), Vladislav STRMISKA (203 Czech Republic), Milica GAGIC (203 Czech Republic), Zuzana HORAKOVA (203 Czech Republic), Rom KOSTRICA (203 Czech Republic), Michal MASAŘÍK (203 Czech Republic, belonging to the institution), Zbynek HEGER (203 Czech Republic) and Vojtech ADAM (203 Czech Republic, guarantor)

Edition

Talanta, AMSTERDAM, Elsevier, 2019, 0039-9140

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30204 Oncology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.339

RIV identification code

RIV/00216224:14110/19:00107803

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.talanta.2019.07.006

UT WoS

000485856500047

Keywords in English

Cancer; Human papillomavirus; Nanotechnology; Magnetic isolation; Quantum dots

Abstract

V originále

Due to the close relationship between carcinogenesis and human papillomavirus (HPV), and since they are transmitted via huge number of asymptomatic carriers, the detection of HPV is really needed to reduce the risk of developing cancer. According to the best of our knowledge, our study provides the very first method for one-step detection of viral infection and if it has initiated the subsequent cancer proliferation. The proposed novel nanosystem consists of magnetic glass particles (MGPs), which were attached with DNA probe on their surface to hybridize with target DNAs. The MGP-probe-DNA hybrid was finally conjugated with CdTe/ZnSe core/shell quantum dots (QDs). The proposed detection system is based on a novel mechanism in which the MGPs separate out the target DNAs from different biological samples using external magnetic field for better and clear detection and the QDs give different fluorescent maxima for different target DNAs due to their ability to interact differently with different nucleotides. Firstly, the method was optimized using HPV genes cloned into synthetic plasmids. Then it was applied directly on the samples from normal and cancerous cells. After that, the real hospital samples of head and neck squamous cell carcinoma (HNSCC) with or without the infection of HPV were also analyzed. Our novel nano-system is proved successful in detecting and distinguishing between the patients suffering by HPV infection with or without subsequent cancer having detection limit estimated as 1.0 x 10(9) (GEq/mL). The proposed methodology is faster and cost-effective, which can be applied at the clinical level to help the doctors to decide the strategy of medication that may save the life of the patients with an early treatment.

Links

GA18-03978S, research and development project

Name: Efekt buněčné tuhosti a mikroprostředí jako prediktor odpovědi na léčbu karcinomů hlavy a krku

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

NV16-29835A, research and development project

Name: Molekulárně-genetické markery predikce účinnosti radioterapie u nádorů hlavy a krku

Citovat

JIMENEZ, Ana Maria Jimenez, Amitava MOULICK, Sukanya BHOWMICK, Vladislav STRMISKA, Milica GAGIC, Zuzana HORAKOVA, Rom KOSTRICA, Michal MASAŘÍK, Zbynek HEGER and Vojtech ADAM. One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity. Talanta. AMSTERDAM: Elsevier, 2019, vol. 205, DEC 1 2019, p. 1-8. ISSN 0039-9140. Available from: https://dx.doi.org/10.1016/j.talanta.2019.07.006.

@article{1582256,
   author = {Jimenez, Ana Maria Jimenez and Moulick, Amitava and Bhowmick, Sukanya and Strmiska, Vladislav and Gagic, Milica and Horakova, Zuzana and Kostrica, Rom and Masařík, Michal and Heger, Zbynek and Adam, Vojtech},
   article_location = {AMSTERDAM},
   article_number = {DEC 1 2019},
   doi = {http://dx.doi.org/10.1016/j.talanta.2019.07.006},
   keywords = {Cancer; Human papillomavirus; Nanotechnology; Magnetic isolation; Quantum dots},
   language = {eng},
   issn = {0039-9140},
   journal = {Talanta},
   title = {One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity},
   url = {http://dx.doi.org/10.1016/j.talanta.2019.07.006},
   volume = {205},
   year = {2019}
}

TY  - JOUR
ID  - 1582256
AU  - Jimenez, Ana Maria Jimenez - Moulick, Amitava - Bhowmick, Sukanya - Strmiska, Vladislav - Gagic, Milica - Horakova, Zuzana - Kostrica, Rom - Masařík, Michal - Heger, Zbynek - Adam, Vojtech
PY  - 2019
TI  - One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity
JF  - Talanta
VL  - 205
IS  - DEC 1 2019
SP  - 1-8
EP  - 1-8
PB  - Elsevier
SN  - 00399140
KW  - Cancer
KW  - Human papillomavirus
KW  - Nanotechnology
KW  - Magnetic isolation
KW  - Quantum dots
UR  - http://dx.doi.org/10.1016/j.talanta.2019.07.006
L2  - http://dx.doi.org/10.1016/j.talanta.2019.07.006
N2  - Due to the close relationship between carcinogenesis and human papillomavirus (HPV), and since they are transmitted via huge number of asymptomatic carriers, the detection of HPV is really needed to reduce the risk of developing cancer. According to the best of our knowledge, our study provides the very first method for one-step detection of viral infection and if it has initiated the subsequent cancer proliferation. The proposed novel nanosystem consists of magnetic glass particles (MGPs), which were attached with DNA probe on their surface to hybridize with target DNAs. The MGP-probe-DNA hybrid was finally conjugated with CdTe/ZnSe core/shell quantum dots (QDs). The proposed detection system is based on a novel mechanism in which the MGPs separate out the target DNAs from different biological samples using external magnetic field for better and clear detection and the QDs give different fluorescent maxima for different target DNAs due to their ability to interact differently with different nucleotides. Firstly, the method was optimized using HPV genes cloned into synthetic plasmids. Then it was applied directly on the samples from normal and cancerous cells. After that, the real hospital samples of head and neck squamous cell carcinoma (HNSCC) with or without the infection of HPV were also analyzed. Our novel nano-system is proved successful in detecting and distinguishing between the patients suffering by HPV infection with or without subsequent cancer having detection limit estimated as 1.0 x 10(9) (GEq/mL). The proposed methodology is faster and cost-effective, which can be applied at the clinical level to help the doctors to decide the strategy of medication that may save the life of the patients with an early treatment.
ER  -

JIMENEZ, Ana Maria Jimenez, Amitava MOULICK, Sukanya BHOWMICK, Vladislav STRMISKA, Milica GAGIC, Zuzana HORAKOVA, Rom KOSTRICA, Michal MASAŘÍK, Zbynek HEGER and Vojtech ADAM. One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity. \textit{Talanta}. AMSTERDAM: Elsevier, 2019, vol.~205, DEC 1 2019, p.~1-8. ISSN~0039-9140. Available from: https://dx.doi.org/10.1016/j.talanta.2019.07.006.

Detailed Information on Publication Record