Prognostic Significance of Serum Free Amino Acids in Head and
Neck Cancers

J 2019

Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers

VŠIANSKÝ, Vít, Markéta SVOBODOVÁ, Jaromír GUMULEC, Natalia CERNEI, Dagmar STERBOVA et. al.

Basic information

Original name

Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers

Authors

VŠIANSKÝ, Vít (203 Czech Republic, belonging to the institution), Markéta SVOBODOVÁ (203 Czech Republic, belonging to the institution), Jaromír GUMULEC (203 Czech Republic, belonging to the institution), Natalia CERNEI (203 Czech Republic), Dagmar STERBOVA (203 Czech Republic), Ondrej ZITKA (203 Czech Republic), Rom KOSTRICA (203 Czech Republic), Pavel SMILEK (203 Czech Republic), Jan PLZAK (203 Czech Republic), Jan BETKA (203 Czech Republic), David KALFERT (203 Czech Republic), Michal MASAŘÍK (203 Czech Republic, belonging to the institution) and Martina RAUDENSKÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Cells, Basel, MDPI, 2019, 2073-4409

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.366

RIV identification code

RIV/00216224:14110/19:00107806

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/cells8050428

UT WoS

000470994400048

Keywords in English

head and neck cancer; blood biomarkers; prognosis; colony forming assay; metabolism; amino acids

Abstract

V originále

Despite distinctive advances in the field of head and neck squamous cell cancer (HNSCC) biomarker discovery, the spectrum of clinically useful prognostic serum biomarkers is limited. As metabolic activities in highly proliferative transformed cells are fundamentally different from those in non-transformed cells, specific shifts in concentration of different metabolites may serve as diagnostic or prognostic markers. Blood amino acids have been identified as promising biomarkers in different cancers before, but little is known about this field in HNSCC. Blood amino acid profiles of 140 HNSCC patients were examined using high-performance liquid chromatography. Cox proportional hazards regression model was used to assess the prognostic value of amino acid concentrations in serum. Colony forming assay was used to identify the effect of amino acids that were significant in Cox proportional hazards regression models on colony forming ability of FaDu and Detroit 562 cell lines. In the multivariable Cox regression model for overall survival (OS), palliative treatment was associated with an unfavourable prognosis while high serum levels of methionine have had a positive prognostic impact. In the relapse-free survival (RFS) multivariable model, methionine was similarly identified as a positive prognostic factor, along with tumor localization in the oropharynx. Oral cavity localization and primary radio(chemo)therapy treatment strategy have been linked to poorer RFS. 1mM serine was shown to support the forming of colonies in both tested HNSCC cell lines. Effect of methionine was exactly the opposite.

Links

GA18-03978S, research and development project

Name: Efekt buněčné tuhosti a mikroprostředí jako prediktor odpovědi na léčbu karcinomů hlavy a krku

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

NV18-08-00229, research and development project

Name: Interakce buněk nádorového mikroprostředí jako nový nástroj predikce účinnosti terapie nádorů hlavy a krku

Investor: Ministry of Health of the CR

ROZV/24/LF/2018, interní kód MU

Name: LF - Příspěvek na IP 2108

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

Citovat

VŠIANSKÝ, Vít, Markéta SVOBODOVÁ, Jaromír GUMULEC, Natalia CERNEI, Dagmar STERBOVA, Ondrej ZITKA, Rom KOSTRICA, Pavel SMILEK, Jan PLZAK, Jan BETKA, David KALFERT, Michal MASAŘÍK and Martina RAUDENSKÁ. Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers. Cells. Basel: MDPI, 2019, vol. 8, No 5, p. 1-12. ISSN 2073-4409. Available from: https://dx.doi.org/10.3390/cells8050428.

@article{1582324,
   author = {Všianský, Vít and Svobodová, Markéta and Gumulec, Jaromír and Cernei, Natalia and Sterbova, Dagmar and Zitka, Ondrej and Kostrica, Rom and Smilek, Pavel and Plzak, Jan and Betka, Jan and Kalfert, David and Masařík, Michal and Raudenská, Martina},
   article_location = {Basel},
   article_number = {5},
   doi = {http://dx.doi.org/10.3390/cells8050428},
   keywords = {head and neck cancer; blood biomarkers; prognosis; colony forming assay; metabolism; amino acids},
   language = {eng},
   issn = {2073-4409},
   journal = {Cells},
   title = {Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers},
   url = {http://dx.doi.org/10.3390/cells8050428},
   volume = {8},
   year = {2019}
}

TY  - JOUR
ID  - 1582324
AU  - Všianský, Vít - Svobodová, Markéta - Gumulec, Jaromír - Cernei, Natalia - Sterbova, Dagmar - Zitka, Ondrej - Kostrica, Rom - Smilek, Pavel - Plzak, Jan - Betka, Jan - Kalfert, David - Masařík, Michal - Raudenská, Martina
PY  - 2019
TI  - Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers
JF  - Cells
VL  - 8
IS  - 5
SP  - 1-12
EP  - 1-12
PB  - MDPI
SN  - 20734409
KW  - head and neck cancer
KW  - blood biomarkers
KW  - prognosis
KW  - colony forming assay
KW  - metabolism
KW  - amino acids
UR  - http://dx.doi.org/10.3390/cells8050428
L2  - http://dx.doi.org/10.3390/cells8050428
N2  - Despite distinctive advances in the field of head and neck squamous cell cancer (HNSCC) biomarker discovery, the spectrum of clinically useful prognostic serum biomarkers is limited. As metabolic activities in highly proliferative transformed cells are fundamentally different from those in non-transformed cells, specific shifts in concentration of different metabolites may serve as diagnostic or prognostic markers. Blood amino acids have been identified as promising biomarkers in different cancers before, but little is known about this field in HNSCC. Blood amino acid profiles of 140 HNSCC patients were examined using high-performance liquid chromatography. Cox proportional hazards regression model was used to assess the prognostic value of amino acid concentrations in serum. Colony forming assay was used to identify the effect of amino acids that were significant in Cox proportional hazards regression models on colony forming ability of FaDu and Detroit 562 cell lines. In the multivariable Cox regression model for overall survival (OS), palliative treatment was associated with an unfavourable prognosis while high serum levels of methionine have had a positive prognostic impact. In the relapse-free survival (RFS) multivariable model, methionine was similarly identified as a positive prognostic factor, along with tumor localization in the oropharynx. Oral cavity localization and primary radio(chemo)therapy treatment strategy have been linked to poorer RFS. 1mM serine was shown to support the forming of colonies in both tested HNSCC cell lines. Effect of methionine was exactly the opposite.
ER  -

VŠIANSKÝ, Vít, Markéta SVOBODOVÁ, Jaromír GUMULEC, Natalia CERNEI, Dagmar STERBOVA, Ondrej ZITKA, Rom KOSTRICA, Pavel SMILEK, Jan PLZAK, Jan BETKA, David KALFERT, Michal MASAŘÍK and Martina RAUDENSKÁ. Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers. \textit{Cells}. Basel: MDPI, 2019, vol.~8, No~5, p.~1-12. ISSN~2073-4409. Available from: https://dx.doi.org/10.3390/cells8050428.

Detailed Information on Publication Record