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@article{1585721,
author = {Makhneva, Ekaterina and Farka, Zdeněk and Pastucha, Matěj and Obrusník, Adam and Horáčková, Veronika and Skládal, Petr and Zajíčková, Lenka},
article_number = {29},
doi = {http://dx.doi.org/10.1007/s00216-019-01979-9},
keywords = {Plasma polymerization; Carboxyl-rich films; Immobilization; Surface plasmon resonance biosensor; Label-free detection},
language = {eng},
issn = {1618-2642},
journal = {Analytical and Bioanalytical Chemistry},
title = {Maleic anhydride and acetylene plasma copolymer surfaces for SPR immunosensing},
url = {https://link.springer.com/article/10.1007/s00216-019-01979-9},
volume = {411},
year = {2019}
}
TY - JOUR
ID - 1585721
AU - Makhneva, Ekaterina - Farka, Zdeněk - Pastucha, Matěj - Obrusník, Adam - Horáčková, Veronika - Skládal, Petr - Zajíčková, Lenka
PY - 2019
TI - Maleic anhydride and acetylene plasma copolymer surfaces for SPR immunosensing
JF - Analytical and Bioanalytical Chemistry
VL - 411
IS - 29
SP - 7689-7697
EP - 7689-7697
PB - Springer-Verlag
SN - 16182642
KW - Plasma polymerization
KW - Carboxyl-rich films
KW - Immobilization
KW - Surface plasmon resonance biosensor
KW - Label-free detection
UR - https://link.springer.com/article/10.1007/s00216-019-01979-9
L2 - https://link.springer.com/article/10.1007/s00216-019-01979-9
N2 - We report on the successful application of carboxyl-rich plasma polymerized (PP) films as a matrix layer for bioreceptor immobilization in surface plasmon resonance (SPR) immunosensing. Composition and chemical properties of the carboxyl-rich PP films deposited from a mixture of maleic anhydride and acetylene were investigated. Changes in the films stored in air, water, and buffer were studied and the involved chemical changes were described. Performance in SPR immunosensing was evaluated on interactions of human serum albumin (HSA) with a specific monoclonal antibody. The comparison with the mixed self-assembled monolayer of mercaptoundecanoic acid and mercaptohexanol (MUA/MCH) and one of the most widely used surfaces for SPR, the 2D and 3D carboxymethylated dextran (CMD), was presented to show the efficacy of plasma polymerized matrix layers for biosensing. The PP film-based SPR immunosensor provided a similar detection limit of HSA (100 ng/mL) as MUA/MCH- (100 ng/mL) and 3D CMD (50 ng/mL)-based sensors. However, the response levels were about twice higher in case of the PP film-based immunosensor than in case of MUA/MCH-based alternative. The PP film surfaces had similar binding capacity towards antibody as the 3D CMD layers. The response of PP film-based sensor towards HSA was comparable to 3D CMD-based sensor up to 2.5 ug/mL. For the higher concentrations (> 10 ug/mL), the response of PP film-based immunosensor was lower due to inaccessibility of active sites of the immobilized antibody inside the flat PP film surface. We have demonstrated that due to its high stability and cost-effective straightforward preparation, the carboxyl-rich PP films represent an efficient alternative to self-assembled monolayers (SAM) and dextran-based layers in label-free immunosensing.
ER -
MAKHNEVA, Ekaterina, Zdeněk FARKA, Matěj PASTUCHA, Adam OBRUSNÍK, Veronika HORÁČKOVÁ, Petr SKLÁDAL a Lenka ZAJÍČKOVÁ. Maleic anhydride and acetylene plasma copolymer surfaces for SPR immunosensing. \textit{Analytical and Bioanalytical Chemistry}. Springer-Verlag, 2019, roč.~411, č.~29, s.~7689-7697. ISSN~1618-2642. Dostupné z: https://dx.doi.org/10.1007/s00216-019-01979-9.
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