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@article{1587716,
author = {Grillová, Linda and Oppelt, Jan and Mikalová, Lenka and Nováková, Markéta and Giacani, LLorenzo and Niesnerová, Anežka and Noda, Angel A. and Mechaly, Ariel E. and Pospíšilová, Petra and Cejkova, Darina and Grange, Philippe A. and Dupin, Nicolas and Strnadel, Radim and Chen, Marcus and Denham, Ian and Arora, Natasha and Picardeau, Mathieu and Weston, Christopher and Forsyth, R. Allyn and Šmajs, David},
article_location = {Lausanne},
article_number = {JUL 31 2019},
doi = {http://dx.doi.org/10.3389/fmicb.2019.01691},
keywords = {Treponema pallidum subsp. pallidum; syphilis; direct whole genome sequencing; recombinationd-riven diversity; culture-independent bacterial enrichment},
language = {eng},
issn = {1664-302X},
journal = {Frontiers in Microbiology},
title = {Directly Sequenced Genomes of Contemporary Strains of Syphilis Reveal Recombination-Driven Diversity in Genes Encoding Predicted Surface-Exposed Antigens},
url = {https://www.frontiersin.org/articles/10.3389/fmicb.2019.01691/full},
volume = {10},
year = {2019}
}
TY - JOUR
ID - 1587716
AU - Grillová, Linda - Oppelt, Jan - Mikalová, Lenka - Nováková, Markéta - Giacani, LLorenzo - Niesnerová, Anežka - Noda, Angel A. - Mechaly, Ariel E. - Pospíšilová, Petra - Cejkova, Darina - Grange, Philippe A. - Dupin, Nicolas - Strnadel, Radim - Chen, Marcus - Denham, Ian - Arora, Natasha - Picardeau, Mathieu - Weston, Christopher - Forsyth, R. Allyn - Šmajs, David
PY - 2019
TI - Directly Sequenced Genomes of Contemporary Strains of Syphilis Reveal Recombination-Driven Diversity in Genes Encoding Predicted Surface-Exposed Antigens
JF - Frontiers in Microbiology
VL - 10
IS - JUL 31 2019
SP - 1-14
EP - 1-14
PB - Frontiers Media SA
SN - 1664302X
KW - Treponema pallidum subsp. pallidum
KW - syphilis
KW - direct whole genome sequencing
KW - recombinationd-riven diversity
KW - culture-independent bacterial enrichment
UR - https://www.frontiersin.org/articles/10.3389/fmicb.2019.01691/full
L2 - https://www.frontiersin.org/articles/10.3389/fmicb.2019.01691/full
N2 - Syphilis, caused by Treponema pallidum subsp. pallidum (TPA), remains an important public health problem with an increasing worldwide prevalence. Despite recent advances in in vitro cultivation, genetic variability of this pathogen during infection is poorly understood. Here, we present contemporary and geographically diverse complete treponemal genome sequences isolated directly from patients using a methyl-directed enrichment prior to sequencing. This approach reveals that approximately 50% of the genetic diversity found in TPA is driven by inter- and/or intra-strain recombination events, particularly in strains belonging to one of the defined genetic groups of syphilis treponemes: Nichols-like strains. Recombinant loci were found to encode putative outer-membrane proteins and the recombination variability was almost exclusively found in regions predicted to be at the host-pathogen interface. Genetic recombination has been considered to be a rare event in treponemes, yet our study unexpectedly showed that it occurs at a significant level and may have important impacts in the biology of this pathogen, especially as these events occur primarily in the outer membrane proteins. This study reveals the existence of strains with different repertoires of surface-exposed antigens circulating in the current human population, which should be taken into account during syphilis vaccine development.
ER -
GRILLOVÁ, Linda, Jan OPPELT, Lenka MIKALOVÁ, Markéta NOVÁKOVÁ, LLorenzo GIACANI, Anežka NIESNEROVÁ, Angel A. NODA, Ariel E. MECHALY, Petra POSPÍŠILOVÁ, Darina CEJKOVA, Philippe A. GRANGE, Nicolas DUPIN, Radim STRNADEL, Marcus CHEN, Ian DENHAM, Natasha ARORA, Mathieu PICARDEAU, Christopher WESTON, R. Allyn FORSYTH a David ŠMAJS. Directly Sequenced Genomes of Contemporary Strains of Syphilis Reveal Recombination-Driven Diversity in Genes Encoding Predicted Surface-Exposed Antigens. \textit{Frontiers in Microbiology}. Lausanne: Frontiers Media SA, roč.~10, JUL 31 2019, s.~1-14. ISSN~1664-302X. doi:10.3389/fmicb.2019.01691. 2019.
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