First report of hare treponematosis seroprevalence of European
brown hares (Lepus europaeus) in the Czech Republic:
seroprevalence negatively correlates with altitude of sampling
areas

J 2019

First report of hare treponematosis seroprevalence of European brown hares (Lepus europaeus) in the Czech Republic: seroprevalence negatively correlates with altitude of sampling areas

NOVÁKOVÁ, Markéta, David NAJT, Lenka PAŠTĚKOVÁ, Marcela KOSTKOVA, Eliška VRBOVÁ et. al.

Basic information

Original name

First report of hare treponematosis seroprevalence of European brown hares (Lepus europaeus) in the Czech Republic: seroprevalence negatively correlates with altitude of sampling areas

Authors

NOVÁKOVÁ, Markéta (203 Czech Republic, belonging to the institution), David NAJT (203 Czech Republic, belonging to the institution), Lenka PAŠTĚKOVÁ (203 Czech Republic, belonging to the institution), Marcela KOSTKOVA (203 Czech Republic), Eliška VRBOVÁ (203 Czech Republic, belonging to the institution), Michal STROUHAL (203 Czech Republic, belonging to the institution), Annika POSAUTZ (40 Austria), Sascha KNAUF (276 Germany) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

BMC Veterinary Research, LONDON, BIOMED CENTRAL LTD, 2019, 1746-6148

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

40301 Veterinary science

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.835

RIV identification code

RIV/00216224:14110/19:00107835

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1186/s12917-019-2086-3

UT WoS

000490993100001

Keywords in English

Hare disease; Venereal disease; Wildlife disease; Game animals; Lesion; Lepus europaeus; Lepus timidus; Lagomorphs; Treponema paraluisleporidarum; Treponematosis

Abstract

V originále

Background The aim of this study was to quantify the seroprevalence of hare treponematosis in European brown hare (Lepus europaeus) populations in the Czech Republic and to test for an association between treponematosis prevalence and the altitude of the areas in which hares were sampled. We tested 289 serum samples of brown hares collected between 2015 and 2017. The sampling areas included 12 districts (73 villages) distributed throughout the Czech Republic. Serum samples were tested for the presence of antibodies against the causative agent of hare treponematosis (Treponema paraluisleporidarum ecovar Lepus, TPeL) using two serological tests for human syphilis that cross-react with TPeL: the Treponema pallidum hemagglutination assay (TPHA) and the fluorescent treponemal antibody absorption (FTA-ABS) test. To account for the imperfect diagnostic sensitivity and specificity of each test, apparent prevalence estimates of TPeL were converted to true prevalence estimates using the Rogan Gladen estimator. The correlation between TPeL true seroprevalence and altitude of sampling areas was analyzed using Pearson's correlation coefficient at three levels of spatial resolution: (1) four groups, each composed of two merged districts, with >= 20 samples collected, differing in their altitude median (206, 348, 495, and 522 m above sea level); (2) separately tested eight districts, where >= 20 samples were collected per district; and (3) 27 groups composed of villages of the same altitude level distributed across the whole dataset. Results One hundred and seven of the 289 samples were seropositive to both tests, the FTA-ABS test was positive for an additional 47 samples. Seropositive samples were found in all 12 districts. True seroprevalence of TPeL in the sampled hares was 52% (95% confidence interval 46 to 58%). A statistically significant negative correlation between TPeL seroprevalence and altitude was identified at the district level (Pearson's r = - 0.722, p = 0.043). Conclusions Between 2015 and 2017 hare treponematosis was present at a relatively high prevalence in brown hares in all 12 districts in the Czech Republic where sampling was carried out. The seroprevalence of TPeL in brown hares was negatively correlated with the altitude of the areas in which hares were sampled.

Links

GC18-23521J, research and development project

Name: Treponematózy u zástupců řádu zajícovci: genetická diverzita treponem a příbuznost s lidským patogenem T. pallidum

Investor: Czech Science Foundation

MUNI/A/1087/2018, interní kód MU

Name: Molekulární a buněčná biologie pro biomedicínské vědy

Investor: Masaryk University, Category A

ROZV/23/LF21/2019, interní kód MU

Name: Stopové prvky ve výživě a zdraví člověka

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

ROZV/24/LF/2018, interní kód MU

Name: LF - Příspěvek na IP 2108

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

Citovat

NOVÁKOVÁ, Markéta, David NAJT, Lenka PAŠTĚKOVÁ, Marcela KOSTKOVA, Eliška VRBOVÁ, Michal STROUHAL, Annika POSAUTZ, Sascha KNAUF and David ŠMAJS. First report of hare treponematosis seroprevalence of European brown hares (Lepus europaeus) in the Czech Republic: seroprevalence negatively correlates with altitude of sampling areas. BMC Veterinary Research. LONDON: BIOMED CENTRAL LTD, 2019, vol. 15, No 1, p. 1-7. ISSN 1746-6148. Available from: https://dx.doi.org/10.1186/s12917-019-2086-3.

@article{1587740,
   author = {Nováková, Markéta and Najt, David and Paštěková, Lenka and Kostkova, Marcela and Vrbová, Eliška and Strouhal, Michal and Posautz, Annika and Knauf, Sascha and Šmajs, David},
   article_location = {LONDON},
   article_number = {1},
   doi = {http://dx.doi.org/10.1186/s12917-019-2086-3},
   keywords = {Hare disease; Venereal disease; Wildlife disease; Game animals; Lesion; Lepus europaeus; Lepus timidus; Lagomorphs; Treponema paraluisleporidarum; Treponematosis},
   language = {eng},
   issn = {1746-6148},
   journal = {BMC Veterinary Research},
   title = {First report of hare treponematosis seroprevalence of European brown hares (Lepus europaeus) in the Czech Republic: seroprevalence negatively correlates with altitude of sampling areas},
   url = {http://dx.doi.org/10.1186/s12917-019-2086-3},
   volume = {15},
   year = {2019}
}

TY  - JOUR
ID  - 1587740
AU  - Nováková, Markéta - Najt, David - Paštěková, Lenka - Kostkova, Marcela - Vrbová, Eliška - Strouhal, Michal - Posautz, Annika - Knauf, Sascha - Šmajs, David
PY  - 2019
TI  - First report of hare treponematosis seroprevalence of European brown hares (Lepus europaeus) in the Czech Republic: seroprevalence negatively correlates with altitude of sampling areas
JF  - BMC Veterinary Research
VL  - 15
IS  - 1
SP  - 1-7
EP  - 1-7
PB  - BIOMED CENTRAL LTD
SN  - 17466148
KW  - Hare disease
KW  - Venereal disease
KW  - Wildlife disease
KW  - Game animals
KW  - Lesion
KW  - Lepus europaeus
KW  - Lepus timidus
KW  - Lagomorphs
KW  - Treponema paraluisleporidarum
KW  - Treponematosis
UR  - http://dx.doi.org/10.1186/s12917-019-2086-3
L2  - http://dx.doi.org/10.1186/s12917-019-2086-3
N2  - Background The aim of this study was to quantify the seroprevalence of hare treponematosis in European brown hare (Lepus europaeus) populations in the Czech Republic and to test for an association between treponematosis prevalence and the altitude of the areas in which hares were sampled. We tested 289 serum samples of brown hares collected between 2015 and 2017. The sampling areas included 12 districts (73 villages) distributed throughout the Czech Republic. Serum samples were tested for the presence of antibodies against the causative agent of hare treponematosis (Treponema paraluisleporidarum ecovar Lepus, TPeL) using two serological tests for human syphilis that cross-react with TPeL: the Treponema pallidum hemagglutination assay (TPHA) and the fluorescent treponemal antibody absorption (FTA-ABS) test. To account for the imperfect diagnostic sensitivity and specificity of each test, apparent prevalence estimates of TPeL were converted to true prevalence estimates using the Rogan Gladen estimator. The correlation between TPeL true seroprevalence and altitude of sampling areas was analyzed using Pearson's correlation coefficient at three levels of spatial resolution: (1) four groups, each composed of two merged districts, with >= 20 samples collected, differing in their altitude median (206, 348, 495, and 522 m above sea level); (2) separately tested eight districts, where >= 20 samples were collected per district; and (3) 27 groups composed of villages of the same altitude level distributed across the whole dataset. Results One hundred and seven of the 289 samples were seropositive to both tests, the FTA-ABS test was positive for an additional 47 samples. Seropositive samples were found in all 12 districts. True seroprevalence of TPeL in the sampled hares was 52% (95% confidence interval 46 to 58%). A statistically significant negative correlation between TPeL seroprevalence and altitude was identified at the district level (Pearson's r = - 0.722, p = 0.043). Conclusions Between 2015 and 2017 hare treponematosis was present at a relatively high prevalence in brown hares in all 12 districts in the Czech Republic where sampling was carried out. The seroprevalence of TPeL in brown hares was negatively correlated with the altitude of the areas in which hares were sampled.
ER  -

NOVÁKOVÁ, Markéta, David NAJT, Lenka PAŠTĚKOVÁ, Marcela KOSTKOVA, Eliška VRBOVÁ, Michal STROUHAL, Annika POSAUTZ, Sascha KNAUF and David ŠMAJS. First report of hare treponematosis seroprevalence of European brown hares (Lepus europaeus) in the Czech Republic: seroprevalence negatively correlates with altitude of sampling areas. \textit{BMC Veterinary Research}. LONDON: BIOMED CENTRAL LTD, 2019, vol.~15, No~1, p.~1-7. ISSN~1746-6148. Available from: https://dx.doi.org/10.1186/s12917-019-2086-3.

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