Capillary electrophoretic analysis of ionic content in exhaled
breath condensate and pH monitoring as a non-invasive method in
gastroesophageal reflux disease diagnostics

J 2019

Capillary electrophoretic analysis of ionic content in exhaled breath condensate and pH monitoring as a non-invasive method in gastroesophageal reflux disease diagnostics

LAČNÁ, Júlia, Pavol ĎURČ, Michal GREGUŠ, Jana SKŘIČKOVÁ, Martina DOUBKOVÁ et. al.

Basic information

Original name

Capillary electrophoretic analysis of ionic content in exhaled breath condensate and pH monitoring as a non-invasive method in gastroesophageal reflux disease diagnostics

Authors

LAČNÁ, Júlia (703 Slovakia, belonging to the institution), Pavol ĎURČ (703 Slovakia, belonging to the institution), Michal GREGUŠ (703 Slovakia, belonging to the institution), Jana SKŘIČKOVÁ (203 Czech Republic, belonging to the institution), Martina DOUBKOVÁ (203 Czech Republic, belonging to the institution), Eva POKOJOVÁ (203 Czech Republic, belonging to the institution), Dagmar KINDLOVÁ (203 Czech Republic, belonging to the institution), Jiří DOLINA (203 Czech Republic, belonging to the institution), Štefan KONEČNÝ (703 Slovakia, belonging to the institution), František FORET (203 Czech Republic, belonging to the institution) and Petr KUBÁŇ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2019, 1570-0232

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30203 Respiratory systems

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.004

RIV identification code

RIV/00216224:14740/19:00108548

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.jchromb.2019.121857

UT WoS

000502882300008

Keywords (in Czech)

Capillary electrophoresis; Contactless conductivity detection; Exhaled breath condensate; Anions and cations; pH; Gastroesophageal reflux disease

Keywords in English

Capillary electrophoresis; Contactless conductivity detection; Exhaled breath condensate; Anions and cations; pH; Gastroesophageal reflux disease

Abstract

V originále

In this study, the ionic profile and pH of exhaled breath condensate (EBC) in a group of patients with acid and weakly acid reflux and no-reflux controls were compared. A portable sampler was used for non-invasive EBC collection from five exhalations. The ionic profile (anions, cations, organic acids) and pH of the collected EBC samples were measured by capillary electrophoresis with contactless conductivity detection and a pH microelectrode, respectively. Several ions were elevated in the patient groups. Sodium cation was elevated in weakly acid reflux (significance level p < 0.01) and acid reflux (p < 0.05) compared to no-reflux controls. Butyrate and propionate were elevated in both acid reflux and weakly acid reflux compared to no-reflux controls (butyrate: p < 0.01, propionate: p < 0.05). The median values of pH (after de-aeration with N2) were also significantly higher (p < 0.01) in groups with acid reflux and weakly acid reflux than in the control group with no reflux. The ionic analysis and simultaneous pH measurement offer a simple, cheap, fast, and non-invasive approach in gastroesophageal reflux disease diagnostics. © 2019 Elsevier B.V.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

NV17-31945A, research and development project

Name: Vývoj neinvazivní diagnostiky extraezofageálních projevů refluxní choroby jícnu

Citovat

LAČNÁ, Júlia, Pavol ĎURČ, Michal GREGUŠ, Jana SKŘIČKOVÁ, Martina DOUBKOVÁ, Eva POKOJOVÁ, Dagmar KINDLOVÁ, Jiří DOLINA, Štefan KONEČNÝ, František FORET and Petr KUBÁŇ. Capillary electrophoretic analysis of ionic content in exhaled breath condensate and pH monitoring as a non-invasive method in gastroesophageal reflux disease diagnostics. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2019, vol. 1134, DEC, p. 1-7. ISSN 1570-0232. Available from: https://dx.doi.org/10.1016/j.jchromb.2019.121857.

@article{1589459,
   author = {Lačná, Júlia and Ďurč, Pavol and Greguš, Michal and Skřičková, Jana and Doubková, Martina and Pokojová, Eva and Kindlová, Dagmar and Dolina, Jiří and Konečný, Štefan and Foret, František and Kubáň, Petr},
   article_number = {DEC},
   doi = {http://dx.doi.org/10.1016/j.jchromb.2019.121857},
   keywords = {Capillary electrophoresis; Contactless conductivity detection; Exhaled breath condensate; Anions and cations; pH; Gastroesophageal reflux disease},
   language = {eng},
   issn = {1570-0232},
   journal = {Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences},
   title = {Capillary electrophoretic analysis of ionic content in exhaled breath condensate and pH monitoring as a non-invasive method in gastroesophageal reflux disease diagnostics},
   url = {https://www.sciencedirect.com/science/article/pii/S1570023219313297?via%3Dihub},
   volume = {1134},
   year = {2019}
}

TY  - JOUR
ID  - 1589459
AU  - Lačná, Júlia - Ďurč, Pavol - Greguš, Michal - Skřičková, Jana - Doubková, Martina - Pokojová, Eva - Kindlová, Dagmar - Dolina, Jiří - Konečný, Štefan - Foret, František - Kubáň, Petr
PY  - 2019
TI  - Capillary electrophoretic analysis of ionic content in exhaled breath condensate and pH monitoring as a non-invasive method in gastroesophageal reflux disease diagnostics
JF  - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
VL  - 1134
IS  - DEC
SP  - 1-7
EP  - 1-7
SN  - 15700232
KW  - Capillary electrophoresis
KW  - Contactless conductivity detection
KW  - Exhaled breath condensate
KW  - Anions and cations
KW  - pH
KW  - Gastroesophageal reflux disease
UR  - https://www.sciencedirect.com/science/article/pii/S1570023219313297?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S1570023219313297?via%3Dihub
N2  - In this study, the ionic profile and pH of exhaled breath condensate (EBC) in a group of patients with acid and weakly acid reflux and no-reflux controls were compared. A portable sampler was used for non-invasive EBC collection from five exhalations. The ionic profile (anions, cations, organic acids) and pH of the collected EBC samples were measured by capillary electrophoresis with contactless conductivity detection and a pH microelectrode, respectively. Several ions were elevated in the patient groups. Sodium cation was elevated in weakly acid reflux (significance level p < 0.01) and acid reflux (p < 0.05) compared to no-reflux controls. Butyrate and propionate were elevated in both acid reflux and weakly acid reflux compared to no-reflux controls (butyrate: p < 0.01, propionate: p < 0.05). The median values of pH (after de-aeration with N2) were also significantly higher (p < 0.01) in groups with acid reflux and weakly acid reflux than in the control group with no reflux. The ionic analysis and simultaneous pH measurement offer a simple, cheap, fast, and non-invasive approach in gastroesophageal reflux disease diagnostics. © 2019 Elsevier B.V.
ER  -

LAČNÁ, Júlia, Pavol ĎURČ, Michal GREGUŠ, Jana SKŘIČKOVÁ, Martina DOUBKOVÁ, Eva POKOJOVÁ, Dagmar KINDLOVÁ, Jiří DOLINA, Štefan KONEČNÝ, František FORET and Petr KUBÁŇ. Capillary electrophoretic analysis of ionic content in exhaled breath condensate and pH monitoring as a non-invasive method in gastroesophageal reflux disease diagnostics. \textit{Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences}. 2019, vol.~1134, DEC, p.~1-7. ISSN~1570-0232. Available from: https://dx.doi.org/10.1016/j.jchromb.2019.121857.

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