Colicin Z, a structurally and functionally novel colicin type
that selectively kills enteroinvasive Escherichia coli and
Shigella strains

J 2019

Colicin Z, a structurally and functionally novel colicin type that selectively kills enteroinvasive Escherichia coli and Shigella strains

MICENKOVÁ, Lenka, Juraj BOSÁK, Jiří KUČERA, Matěj HRALA, Tereza DOLEJSOVA et. al.

Basic information

Original name

Colicin Z, a structurally and functionally novel colicin type that selectively kills enteroinvasive Escherichia coli and Shigella strains

Authors

MICENKOVÁ, Lenka (703 Slovakia, belonging to the institution), Juraj BOSÁK (703 Slovakia, belonging to the institution), Jiří KUČERA (203 Czech Republic, belonging to the institution), Matěj HRALA (203 Czech Republic, belonging to the institution), Tereza DOLEJSOVA (203 Czech Republic), Ondrej ŠEDO (203 Czech Republic, belonging to the institution), Dirk LINKE (578 Norway), Radovan FISER (203 Czech Republic) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

Scientific reports, LONDON, NATURE PUBLISHING GROUP, 2019, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.998

RIV identification code

RIV/00216224:14110/19:00107866

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1038/s41598-019-47488-8

UT WoS

000477950800050

Keywords in English

NUCLEOTIDE-SEQUENCE; BACTERIAL; IMMUNITY; DOMAIN; GENES; ORGANIZATION; PYOCINS; IMPORT; DNA

Abstract

V originále

Colicin production in Escherichia coli (E. coli) strains represents an important trait with regard to microbial survival and competition in the complex intestinal environment. A novel colicin type, colicin Z (26.3 kDa), was described as a product of an original producer, extraintestinal E. coli B1356 strain, isolated from the anorectal abscess of a 17 years-old man. The 4,007 bp plasmid (pColZ) was completely sequenced and colicin Z activity (cza) and colicin Z immunity (czi) genes were identified. The cza and czi genes are transcribed in opposite directions and encode for 237 and 151 amino acid-long proteins, respectively. Colicin Z shows a narrow inhibitory spectrum, being active only against enteroinvasive E. coli (EIEC) and Shigella strains via CjrC receptor recognition and CjrB- and ExbB-, ExbD-mediated colicin translocation. All tested EIEC and Shigella strains isolated between the years 1958-2010 were sensitive to colicin Z. The lethal effect of colicin Z was found to be directed against cell wall peptidoglycan (PG) resulting in PG degradation, as revealed by experiments with Remazol Brilliant Blue-stained purified peptidoglycans and with MALDI-TOF MS analyses of treated PG. Colicin Z represents a new class of colicins that is structurally and functionally distinct from previously studied colicin types.

Links

EF16_013/0001761, research and development project

Name: RECETOX RI

EF16_013/0001776, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii pro lidské zdraví

GA16-21649S, research and development project

Name: Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

Investor: Czech Science Foundation

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/1087/2018, interní kód MU

Name: Molekulární a buněčná biologie pro biomedicínské vědy

Investor: Masaryk University, Category A

ROZV/24/LF/2018, interní kód MU

Name: LF - Příspěvek na IP 2108

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

90043, large research infrastructures

Name: CIISB

Citovat

MICENKOVÁ, Lenka, Juraj BOSÁK, Jiří KUČERA, Matěj HRALA, Tereza DOLEJSOVA, Ondrej ŠEDO, Dirk LINKE, Radovan FISER and David ŠMAJS. Colicin Z, a structurally and functionally novel colicin type that selectively kills enteroinvasive Escherichia coli and Shigella strains. Scientific reports. LONDON: NATURE PUBLISHING GROUP, 2019, vol. 9, JUL 31 2019, p. 1-12. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-019-47488-8.

@article{1592820,
   author = {Micenková, Lenka and Bosák, Juraj and Kučera, Jiří and Hrala, Matěj and Dolejsova, Tereza and Šedo, Ondrej and Linke, Dirk and Fiser, Radovan and Šmajs, David},
   article_location = {LONDON},
   article_number = {JUL 31 2019},
   doi = {http://dx.doi.org/10.1038/s41598-019-47488-8},
   keywords = {NUCLEOTIDE-SEQUENCE; BACTERIAL; IMMUNITY; DOMAIN; GENES; ORGANIZATION; PYOCINS; IMPORT; DNA},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific reports},
   title = {Colicin Z, a structurally and functionally novel colicin type that selectively kills enteroinvasive Escherichia coli and Shigella strains},
   url = {https://www.nature.com/articles/s41598-019-47488-8.pdf},
   volume = {9},
   year = {2019}
}

TY  - JOUR
ID  - 1592820
AU  - Micenková, Lenka - Bosák, Juraj - Kučera, Jiří - Hrala, Matěj - Dolejsova, Tereza - Šedo, Ondrej - Linke, Dirk - Fiser, Radovan - Šmajs, David
PY  - 2019
TI  - Colicin Z, a structurally and functionally novel colicin type that selectively kills enteroinvasive Escherichia coli and Shigella strains
JF  - Scientific reports
VL  - 9
IS  - JUL 31 2019
SP  - 1-12
EP  - 1-12
PB  - NATURE PUBLISHING GROUP
SN  - 20452322
KW  - NUCLEOTIDE-SEQUENCE
KW  - BACTERIAL
KW  - IMMUNITY
KW  - DOMAIN
KW  - GENES
KW  - ORGANIZATION
KW  - PYOCINS
KW  - IMPORT
KW  - DNA
UR  - https://www.nature.com/articles/s41598-019-47488-8.pdf
L2  - https://www.nature.com/articles/s41598-019-47488-8.pdf
N2  - Colicin production in Escherichia coli (E. coli) strains represents an important trait with regard to microbial survival and competition in the complex intestinal environment. A novel colicin type, colicin Z (26.3 kDa), was described as a product of an original producer, extraintestinal E. coli B1356 strain, isolated from the anorectal abscess of a 17 years-old man. The 4,007 bp plasmid (pColZ) was completely sequenced and colicin Z activity (cza) and colicin Z immunity (czi) genes were identified. The cza and czi genes are transcribed in opposite directions and encode for 237 and 151 amino acid-long proteins, respectively. Colicin Z shows a narrow inhibitory spectrum, being active only against enteroinvasive E. coli (EIEC) and Shigella strains via CjrC receptor recognition and CjrB- and ExbB-, ExbD-mediated colicin translocation. All tested EIEC and Shigella strains isolated between the years 1958-2010 were sensitive to colicin Z. The lethal effect of colicin Z was found to be directed against cell wall peptidoglycan (PG) resulting in PG degradation, as revealed by experiments with Remazol Brilliant Blue-stained purified peptidoglycans and with MALDI-TOF MS analyses of treated PG. Colicin Z represents a new class of colicins that is structurally and functionally distinct from previously studied colicin types.
ER  -

MICENKOVÁ, Lenka, Juraj BOSÁK, Jiří KUČERA, Matěj HRALA, Tereza DOLEJSOVA, Ondrej ŠEDO, Dirk LINKE, Radovan FISER and David ŠMAJS. Colicin Z, a structurally and functionally novel colicin type that selectively kills enteroinvasive Escherichia coli and Shigella strains. \textit{Scientific reports}. LONDON: NATURE PUBLISHING GROUP, 2019, vol.~9, JUL 31 2019, p.~1-12. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-019-47488-8.

Detailed Information on Publication Record