Gelsolin interacts with LamR, hnRNP U, nestin, Arp3 and
beta-tubulin in human melanoma cells as revealed by
immunoprecipitation and mass spectrometry

J 2016

Gelsolin interacts with LamR, hnRNP U, nestin, Arp3 and beta-tubulin in human melanoma cells as revealed by immunoprecipitation and mass spectrometry

MAZUR, Antonina Joanna, Tomasz Witold RADASZKIEWICZ, Aleksandra MAKOWIECKA, Maria MALICKA-BLASZKIEWICZ, Hans Georg MANNHERZ et. al.

Basic information

Original name

Gelsolin interacts with LamR, hnRNP U, nestin, Arp3 and beta-tubulin in human melanoma cells as revealed by immunoprecipitation and mass spectrometry

Authors

MAZUR, Antonina Joanna, Tomasz Witold RADASZKIEWICZ (616 Poland, belonging to the institution), Aleksandra MAKOWIECKA, Maria MALICKA-BLASZKIEWICZ, Hans Georg MANNHERZ and Dorota NOWAK

Edition

European Journal of Cell Biology, Jena, Elsevier GMBH, 2016, 0171-9335

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Full Text

Impact factor

Impact factor: 3.712

RIV identification code

RIV/00216224:14310/16:00111736

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.ejcb.2015.11.001

UT WoS

000371563200003

Keywords in English

Gelsolin; actbl2; LamR; hnRNP U; Nestin; beta Tubulin

Abstract

V originále

Gelsolin, a multifunctional actin binding protein, plays a not yet fully understood role in tumorigenesis. Therefore the goal of this study was to identify additional molecular partners of gelsolin in human melanoma cells, separately in the cytoplasmic compartment and cell nuclei. For this purpose we performed immunoprecipitation experiments based on a modified protocol followed by mass spectrometry. The obtained results were confirmed by Western blot analysis, proximity ligation assays and confocal microscopy. As expected gelsolin interacted with actin, in particular we demonstrate its interaction with cytoplasmic beta and gamma actins, and a newly discovered actin isoform, actbl2. As new gelsolin-interacting partners we identified the ribosomal protein Rpsa, also known as a non-integrin laminin receptor (LamR), and the heterogeneous nuclear ribonucleoprotein hnRNP U. Our data furthermore indicate that gelsolin interacts with particular components of the three cytoskeleton systems: nestin (intermediate filaments), Arp3 (actin cytoskeleton) and beta-tubulin (microtubules). We also report for the first time that gelsolin is a constituent of midbodies, a tubulin containing structure formed at the end of cytokinesis.

Citovat

MAZUR, Antonina Joanna, Tomasz Witold RADASZKIEWICZ, Aleksandra MAKOWIECKA, Maria MALICKA-BLASZKIEWICZ, Hans Georg MANNHERZ and Dorota NOWAK. Gelsolin interacts with LamR, hnRNP U, nestin, Arp3 and beta-tubulin in human melanoma cells as revealed by immunoprecipitation and mass spectrometry. European Journal of Cell Biology. Jena: Elsevier GMBH, 2016, vol. 95, No 1, p. 26-41. ISSN 0171-9335. Available from: https://dx.doi.org/10.1016/j.ejcb.2015.11.001.

@article{1592978,
   author = {Mazur, Antonina Joanna and Radaszkiewicz, Tomasz Witold and Makowiecka, Aleksandra and MalickaandBlaszkiewicz, Maria and Mannherz, Hans Georg and Nowak, Dorota},
   article_location = {Jena},
   article_number = {1},
   doi = {http://dx.doi.org/10.1016/j.ejcb.2015.11.001},
   keywords = {Gelsolin; actbl2; LamR; hnRNP U; Nestin; beta Tubulin},
   language = {eng},
   issn = {0171-9335},
   journal = {European Journal of Cell Biology},
   title = {Gelsolin interacts with LamR, hnRNP U, nestin, Arp3 and beta-tubulin in human melanoma cells as revealed by immunoprecipitation and mass spectrometry},
   url = {https://www.sciencedirect.com/science/article/pii/S0171933515300182?via%3Dihub},
   volume = {95},
   year = {2016}
}

TY  - JOUR
ID  - 1592978
AU  - Mazur, Antonina Joanna - Radaszkiewicz, Tomasz Witold - Makowiecka, Aleksandra - Malicka-Blaszkiewicz, Maria - Mannherz, Hans Georg - Nowak, Dorota
PY  - 2016
TI  - Gelsolin interacts with LamR, hnRNP U, nestin, Arp3 and beta-tubulin in human melanoma cells as revealed by immunoprecipitation and mass spectrometry
JF  - European Journal of Cell Biology
VL  - 95
IS  - 1
SP  - 26-41
EP  - 26-41
PB  - Elsevier GMBH
SN  - 01719335
KW  - Gelsolin
KW  - actbl2
KW  - LamR
KW  - hnRNP U
KW  - Nestin
KW  - beta Tubulin
UR  - https://www.sciencedirect.com/science/article/pii/S0171933515300182?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0171933515300182?via%3Dihub
N2  - Gelsolin, a multifunctional actin binding protein, plays a not yet fully understood role in tumorigenesis. Therefore the goal of this study was to identify additional molecular partners of gelsolin in human melanoma cells, separately in the cytoplasmic compartment and cell nuclei. For this purpose we performed immunoprecipitation experiments based on a modified protocol followed by mass spectrometry. The obtained results were confirmed by Western blot analysis, proximity ligation assays and confocal microscopy. As expected gelsolin interacted with actin, in particular we demonstrate its interaction with cytoplasmic beta and gamma actins, and a newly discovered actin isoform, actbl2. As new gelsolin-interacting partners we identified the ribosomal protein Rpsa, also known as a non-integrin laminin receptor (LamR), and the heterogeneous nuclear ribonucleoprotein hnRNP U. Our data furthermore indicate that gelsolin interacts with particular components of the three cytoskeleton systems: nestin (intermediate filaments), Arp3 (actin cytoskeleton) and beta-tubulin (microtubules). We also report for the first time that gelsolin is a constituent of midbodies, a tubulin containing structure formed at the end of cytokinesis.
ER  -

MAZUR, Antonina Joanna, Tomasz Witold RADASZKIEWICZ, Aleksandra MAKOWIECKA, Maria MALICKA-BLASZKIEWICZ, Hans Georg MANNHERZ and Dorota NOWAK. Gelsolin interacts with LamR, hnRNP U, nestin, Arp3 and beta-tubulin in human melanoma cells as revealed by immunoprecipitation and mass spectrometry. \textit{European Journal of Cell Biology}. Jena: Elsevier GMBH, 2016, vol.~95, No~1, p.~26-41. ISSN~0171-9335. Available from: https://dx.doi.org/10.1016/j.ejcb.2015.11.001.

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