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@article{1593819,
author = {Pešl, Martin and Jelínková, Šárka and Caluori, Guido and Holická, Mária and Krejčí, Jan and Němec, Petr and Kohutová, Aneta and Žampachová, Víta and Dvořák, Petr and Rotrekl, Vladimír},
article_location = {London},
article_number = {1},
doi = {http://dx.doi.org/10.1186/s13023-019-1257-4},
keywords = {Becker muscular dystrophy; Dystrophin; Cardiovascular progenitor cells; C-kit; Cardiomyopathy; Heart failure},
language = {eng},
issn = {1750-1172},
journal = {Orphanet Journal of Rare Diseases},
title = {Cardiovascular progenitor cells and tissue plasticity are reduced in a myocardium affected by Becker muscular dystrophy},
url = {https://ojrd.biomedcentral.com/track/pdf/10.1186/s13023-019-1257-4},
volume = {15},
year = {2020}
}
TY - JOUR
ID - 1593819
AU - Pešl, Martin - Jelínková, Šárka - Caluori, Guido - Holická, Mária - Krejčí, Jan - Němec, Petr - Kohutová, Aneta - Žampachová, Víta - Dvořák, Petr - Rotrekl, Vladimír
PY - 2020
TI - Cardiovascular progenitor cells and tissue plasticity are reduced in a myocardium affected by Becker muscular dystrophy
JF - Orphanet Journal of Rare Diseases
VL - 15
IS - 1
SP - 1-8
EP - 1-8
PB - England
SN - 17501172
KW - Becker muscular dystrophy
KW - Dystrophin
KW - Cardiovascular progenitor cells
KW - C-kit
KW - Cardiomyopathy
KW - Heart failure
UR - https://ojrd.biomedcentral.com/track/pdf/10.1186/s13023-019-1257-4
L2 - https://ojrd.biomedcentral.com/track/pdf/10.1186/s13023-019-1257-4
N2 - We describe the association of Becker muscular dystrophy (BMD) derived heart failure with the impairment of tissue homeostasis and remodeling capabilities of the affected heart tissue. We report that BMD heart failure is associated with a significantly decreased number of cardiovascular progenitor cells, reduced cardiac fibroblast migration, and ex vivo survival. Background Becker muscular dystrophy belongs to a class of genetically inherited dystrophin deficiencies. It affects male patients and results in progressive skeletal muscle degeneration and dilated cardiomyopathy leading to heart failure. It is a relatively mild form of dystrophin deficiency, which allows patients to be on a heart transplant list. In this unique situation, the explanted heart is a rare opportunity to study the degenerative process of dystrophin-deficient cardiac tissue. Heart tissue was excised, dissociated, and analyzed. The fractional content of c-kit(+)/CD45(-) cardiovascular progenitor cells (CVPCs) and cardiac fibroblast migration were compared to control samples of atrial tissue. Control tissue was obtained from the hearts of healthy organ donor's during heart transplantation procedures. Results We report significantly decreased CVPCs (c-kit(+)/CD45(-)) throughout the heart tissue of a BMD patient, and reduced numbers of phase-bright cells presenting c-kit positivity in the dystrophin-deficient cultured explants. In addition, ex vivo CVPCs survival and cardiac fibroblasts migration were significantly reduced, suggesting reduced homeostatic support and irreversible tissue remodeling. Conclusions Our findings associate genetically derived heart failure in a dystrophin-deficient patient with decreased c-kit(+)/CD45(-) CVPCs and their resilience, possibly hinting at a lack of cardioprotective capability and/or reduced homeostatic support. This also correlates with reduced plasticity of the explanted cardiac tissue, related to the process of irreversible remodeling in the BMD patient's heart.
ER -
PEŠL, Martin, Šárka JELÍNKOVÁ, Guido CALUORI, Mária HOLICKÁ, Jan KREJČÍ, Petr NĚMEC, Aneta KOHUTOVÁ, Víta ŽAMPACHOVÁ, Petr DVOŘÁK a Vladimír ROTREKL. Cardiovascular progenitor cells and tissue plasticity are reduced in a myocardium affected by Becker muscular dystrophy. \textit{Orphanet Journal of Rare Diseases}. London: England, 2020, roč.~15, č.~1, s.~1-8. ISSN~1750-1172. Dostupné z: https://dx.doi.org/10.1186/s13023-019-1257-4.
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