Other formats:
BibTeX
LaTeX
RIS
@article{1594177,
author = {Kratochvílová, Irena and Kopečná, O and Bačíková, A and Pagáčová, E and Falková, I and Follett, S E and Elliott, K W Varga K and Golan, M and Falk, Martin},
article_location = {WASHINGTON},
article_number = {23},
doi = {http://dx.doi.org/10.1021/acs.langmuir.8b02742},
language = {eng},
issn = {0743-7463},
journal = {Langmuir},
note = {Žádný z autorů nemá afiliaci k MU, nelze tedy výsledek přenášet do RIV.},
title = {Changes in Cryopreserved Cell Nuclei Serve as Indicators of Processes during Freezing andThawing},
url = {http://dx.doi.org/10.1021/acs.langmuir.8b02742},
volume = {35},
year = {2019}
}
TY - JOUR
ID - 1594177
AU - Kratochvílová, Irena - Kopečná, O - Bačíková, A - Pagáčová, E - Falková, I - Follett, S E - Elliott, K W Varga K - Golan, M - Falk, Martin
PY - 2019
TI - Changes in Cryopreserved Cell Nuclei Serve as Indicators of Processes during Freezing andThawing
JF - Langmuir
VL - 35
IS - 23
SP - 7496-7508
EP - 7496-7508
PB - AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
SN - 07437463
N1 - Žádný z autorů nemá afiliaci k MU, nelze tedy výsledek přenášet do RIV.
UR - http://dx.doi.org/10.1021/acs.langmuir.8b02742
L2 - http://dx.doi.org/10.1021/acs.langmuir.8b02742
N2 - The mechanisms underlying cell protection from cryoinjury are not yet fully understood. Recent biological studies have addressed cryopreserved cell survival but have not correlated the cryoprotection effectiveness with the impact of cryoprotectants on the most important cell structure, the nucleus, and the freeze/thaw process. We identified changes of cell nuclei states caused by different types of cryoprotectants and associate them with alterations of the freeze/thaw process in cells. Namely, we investigated both higher-order chromatin structure and nuclear envelope integrity as possible markers of freezing and thawing processes. Moreover, we analyzed in detail the relationship between nuclear envelope integrity, chromatin condensation, freeze/thaw processes in cells, and cryopreservation efficiency for dimethyl sulfoxide, glycerol, trehalose, and antifreeze protein. Our interdisciplinary study reveals how changes in cell nuclei induced by cryoprotectants affect the ability of cells to withstand freezing and thawing and how nuclei changes correlate with processes during freezing and thawing. Our results contribute to the deeper fundamental understanding of the freezing processes, notably in the cell nucleus, which will expand the applications and lead to the rational design of cryoprotective materials and protocols.
ER -
KRATOCHVÍLOVÁ, Irena, O KOPEČNÁ, A BAČÍKOVÁ, E PAGÁČOVÁ, I FALKOVÁ, S E FOLLETT, K W Varga K ELLIOTT, M GOLAN and Martin FALK. Changes in Cryopreserved Cell Nuclei Serve as Indicators of Processes during Freezing andThawing. \textit{Langmuir}. WASHINGTON: AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA, 2019, vol.~35, No~23, p.~7496-7508. ISSN~0743-7463. Available from: https://dx.doi.org/10.1021/acs.langmuir.8b02742.
|
