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@article{1594201,
author = {Depeš, Daniel and Lee, JinandHo and Bobkova, Elizaveta and Jezkova, Lucie and Falkova, Iva and Bestvater, Felix and Pagacova, Eva and Kopecna, Olga and Zadneprianetc, Mariia and Bacikova, Alena and Kulikova, Elena and Smirnova, Elena and Bulanova, Tatiana and Boreyko, Alla and Krasavin, Evgeny and Hausmann, Michael and Falk, Martin},
article_location = {New York},
article_number = {9},
doi = {http://dx.doi.org/10.1140/epjd/e2018-90148-1},
keywords = {DOUBLE-STRAND BREAKS; COMPLEX CELL RESPONSES; CLUSTERED DNA-DAMAGE; HIGH-LET IRRADIATION; NANOSCOPY TECHNIQUES; ELECTRON-MICROSCOPY; CHROMATIN-STRUCTURE; MULTISCALE APPROACH; RADIATION-DAMAGE; REPAIR},
language = {eng},
issn = {1434-6060},
journal = {The European Physical Journal D},
title = {Single-molecule localization microscopy as a promising tool for gamma H2AX/53BP1 foci exploration},
url = {https://link.springer.com/article/10.1140%2Fepjd%2Fe2018-90148-1},
volume = {72},
year = {2018}
}
TY - JOUR
ID - 1594201
AU - Depeš, Daniel - Lee, Jin-Ho - Bobkova, Elizaveta - Jezkova, Lucie - Falkova, Iva - Bestvater, Felix - Pagacova, Eva - Kopecna, Olga - Zadneprianetc, Mariia - Bacikova, Alena - Kulikova, Elena - Smirnova, Elena - Bulanova, Tatiana - Boreyko, Alla - Krasavin, Evgeny - Hausmann, Michael - Falk, Martin
PY - 2018
TI - Single-molecule localization microscopy as a promising tool for gamma H2AX/53BP1 foci exploration
JF - The European Physical Journal D
VL - 72
IS - 9
SP - 1-11
EP - 1-11
PB - Springer
SN - 14346060
KW - DOUBLE-STRAND BREAKS
KW - COMPLEX CELL RESPONSES
KW - CLUSTERED DNA-DAMAGE
KW - HIGH-LET IRRADIATION
KW - NANOSCOPY TECHNIQUES
KW - ELECTRON-MICROSCOPY
KW - CHROMATIN-STRUCTURE
KW - MULTISCALE APPROACH
KW - RADIATION-DAMAGE
KW - REPAIR
UR - https://link.springer.com/article/10.1140%2Fepjd%2Fe2018-90148-1
L2 - https://link.springer.com/article/10.1140%2Fepjd%2Fe2018-90148-1
N2 - Quantification and structural studies of DNA double strand breaks (DSBs) are an essential part of radiobiology because DSBs represent the most serious damage introduced to the DNA molecule by ionizing radiation. Although standard immunofluorescence confocal microscopy has demonstrated its usefulness in a large number of research studies, it lacks the resolution required to separate individual, closely associated DSBs, which appear after cell exposure to high linear energy transfer (high-LET) radiation and can be visualized as clusters or streaks of radiation-induced repair foci (IRIFs). This prevents our deeper understanding of DSB induction and repair. Recent breakthroughs in super-resolution light microscopy, such as the development of single-molecule localization microscopy (SMLM), offer an optical resolution of approximately an order of magnitude better than that of standard confocal microscopy and open new horizons in radiobiological research. Unlike electron microscopy, SMLM (also referred to as "nanoscopy") preserves the natural structure of biological samples and is not limited to very thin sample slices. Importantly, SMLM not only offers a resolution on the order of approximately 10 nm, but it also provides entirely new information on the biochemistry and spatio-temporal organization of DSBs and DSB repair at the molecular level. Nevertheless, it is still challenging to correctly interpret these often surprising nanoscopy results. In the present article, we describe our first attempts to use SMLM to explore gamma H2AX and 53BP1 repair foci induced with( 15) N high-LET particles.
ER -
DEPEŠ, Daniel, Jin-Ho LEE, Elizaveta BOBKOVA, Lucie JEZKOVA, Iva FALKOVA, Felix BESTVATER, Eva PAGACOVA, Olga KOPECNA, Mariia ZADNEPRIANETC, Alena BACIKOVA, Elena KULIKOVA, Elena SMIRNOVA, Tatiana BULANOVA, Alla BOREYKO, Evgeny KRASAVIN, Michael HAUSMANN and Martin FALK. Single-molecule localization microscopy as a promising tool for gamma H2AX/53BP1 foci exploration. \textit{The European Physical Journal D}. New York: Springer, 2018, vol.~72, No~9, p.~1-11. ISSN~1434-6060. Available from: https://dx.doi.org/10.1140/epjd/e2018-90148-1.
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