| KRATOCHVÍLOVÁ, I, M GOLAN, K POMEISL, J RICHTER, S SEDLÁKOVÁ, J ŠEBERA, J MIČOVÁ, Martin FALK, I FALKOVÁ, D ŘEHA, K W ELLIOTT, K VARGA, S E FOLLETT and D ŠIMEK. Theoretical and experimental study of the antifreeze protein AFP752, trehalose and dimethylsulfoxide cryoprotection mechanism: correlation with cryopreserved cell viability. RSC Advances. Cambridge: Royal Society of Chemistry, 2017, vol. 7, No 1, p. 352-360. ISSN 2046-2069. Available from: https://dx.doi.org/10.1039/c6ra25095e. |
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@article{1594210,
author = {Kratochvílová, I and Golan, M and Pomeisl, K and Richter, J and Sedláková, S and Šebera, J and Mičová, J and Falk, Martin and Falková, I and Řeha, D and Elliott, K W and Varga, K and Follett, S E and Šimek, D},
article_location = {Cambridge},
article_number = {1},
doi = {http://dx.doi.org/10.1039/c6ra25095e},
language = {eng},
issn = {2046-2069},
journal = {RSC Advances},
title = {Theoretical and experimental study of the antifreeze protein AFP752, trehalose and dimethylsulfoxide cryoprotection mechanism: correlation with cryopreserved cell viability},
url = {http://dx.doi.org/10.1039/c6ra25095e},
volume = {7},
year = {2017}
}
TY - JOUR
ID - 1594210
AU - Kratochvílová, I - Golan, M - Pomeisl, K - Richter, J - Sedláková, S - Šebera, J - Mičová, J - Falk, Martin - Falková, I - Řeha, D - Elliott, K W - Varga, K - Follett, S E - Šimek, D
PY - 2017
TI - Theoretical and experimental study of the antifreeze protein AFP752, trehalose and dimethylsulfoxide cryoprotection mechanism: correlation with cryopreserved cell viability
JF - RSC Advances
VL - 7
IS - 1
SP - 352-360
EP - 352-360
PB - Royal Society of Chemistry
SN - 20462069
UR - http://dx.doi.org/10.1039/c6ra25095e
L2 - http://dx.doi.org/10.1039/c6ra25095e
N2 - In this work the physico-chemical properties of selected cryoprotectants (antifreeze protein TrxA-AFP752, trehalose and dimethyl sulfoxide) were correlated with their impact on the constitution of ice and influence on frozen/thawed cell viability. The freezing processes and states of investigated materials solutions were described and explained from a fundamental point of view using ab initio modelling (molecular dynamics, DFT), Raman spectroscopy, differential scanning calorimetry and X-ray diffraction. For the first time, in this work we correlated the microscopic view (modelling) with the description of the frozen solution states and put these results in the context of human skin fibroblast viability after freezing and thawing. DMSO and AFP had different impacts on their solution's freezing process but in both cases the ice crystallinity size was considerably reduced. DMSO and AFP treatment in different ways improved the viability of frozen/thawed cells.
ER -
KRATOCHVÍLOVÁ, I, M GOLAN, K POMEISL, J RICHTER, S SEDLÁKOVÁ, J ŠEBERA, J MIČOVÁ, Martin FALK, I FALKOVÁ, D ŘEHA, K W ELLIOTT, K VARGA, S E FOLLETT and D ŠIMEK. Theoretical and experimental study of the antifreeze protein AFP752, trehalose and dimethylsulfoxide cryoprotection mechanism: correlation with cryopreserved cell viability. \textit{RSC Advances}. Cambridge: Royal Society of Chemistry, 2017, vol.~7, No~1, p.~352-360. ISSN~2046-2069. Available from: https://dx.doi.org/10.1039/c6ra25095e.
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