In the present study, the POx layer was deposited on the plasma-activated PTFE surface and subsequently post-treated by cold DCSBD plasma generated either in an atmosphere of ambient air or argon at atmospheric pressure. Scanning electron microscopy, as well as X-ray photoelectron spectroscopy, confirmed the presence of a thin POx layer on the PTFE surface. We observed the decreasing of water contact angle from an initial value of 105.6° ± 3.9° to values within the range of 55-65° that remained stable for one month. In comparison to pristine PTFE, which does not promote the cell adhesion, the presence of POx layer on the surface increased cell adhesion, which is beneficial, for example, in the tissue engineering applications or regenerative medicine. Moreover, this study provides a new approach for the preparation of POx coatings, which is faster, easier, and more economically friendly in comparison to traditional grafting methods.