Novel synergistic culturing approach for efficient and robust
differentiation of human pluripotent stem cells into
endothelial progenitors.

a 2019

Novel synergistic culturing approach for efficient and robust differentiation of human pluripotent stem cells into endothelial progenitors.

FARKAŠ, Šimon, Pavel ŠIMARA, Daniela ŘEHÁKOVÁ, Tereza SOURALOVÁ, Irena KOUTNÁ et. al.

Basic information

Original name

Novel synergistic culturing approach for efficient and robust differentiation of human pluripotent stem cells into endothelial progenitors.

Authors

FARKAŠ, Šimon (703 Slovakia, belonging to the institution), Pavel ŠIMARA (203 Czech Republic, guarantor, belonging to the institution), Daniela ŘEHÁKOVÁ (203 Czech Republic, belonging to the institution), Tereza SOURALOVÁ (203 Czech Republic, belonging to the institution) and Irena KOUTNÁ (203 Czech Republic, belonging to the institution)

Edition

8. ročník konference Stem Cells and Cell Therapy: From Research to Modern Clinical Applications, Lednice, Czech Republic, 2019

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10601 Cell biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14110/19:00108561

Organization unit

Faculty of Medicine

Keywords (in Czech)

pluripotentní kmenové buňky; endotheliální diferenciace

Keywords in English

pluripotent stem cells; endothelial differentiation

Abstract

V originále

Cellular therapy is a powerful tool for treating various human diseases and reparation of the damaged vasculature is a specialized subtype of this therapy. Using human pluripotent stem cells (hPSCs) as a starting material, it should be possible to generate high amounts of endothelial progenitor cells (EPCs). Our aim was to develop highly efficient, robust and easily reproducible differentiation protocol that would allow us to produce these high amounts of EPCs. Presented protocol follows proper physiological pathway by differentiating hPSCs in three phases through primitive streak and kinase insert domain receptor (KDR) positive mesoderm into EPCs. The differentiation protocol only takes 5 days and results in high amounts of pure EPCs. The robustness is demonstrated by using both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). Better efficiency and lesser line-to-line variability were observed for our differentiation protocol in comparison to other published protocols. Furthermore, derived EPCs expressed high proliferative potential EPC marker CD157 on their surface in addition to standard EPC markers CD31, CD144, CD34, KDR and C-X-C chemokine receptor type 4 (CXCR4).

Links

NV16-31501A, research and development project

Name: Tkáňové inženýrství epitelů: Buňky a protokoly pro regenerativní medicínu

Citovat

FARKAŠ, Šimon, Pavel ŠIMARA, Daniela ŘEHÁKOVÁ, Tereza SOURALOVÁ and Irena KOUTNÁ. Novel synergistic culturing approach for efficient and robust differentiation of human pluripotent stem cells into endothelial progenitors. In 8. ročník konference Stem Cells and Cell Therapy: From Research to Modern Clinical Applications, Lednice, Czech Republic. 2019.

@proceedings{1599660,
   author = {Farkaš, Šimon and Šimara, Pavel and Řeháková, Daniela and Souralová, Tereza and Koutná, Irena},
   booktitle = {8. ročník konference Stem Cells and Cell Therapy: From Research to Modern Clinical Applications, Lednice, Czech Republic},
   keywords = {pluripotent stem cells; endothelial differentiation},
   language = {eng},
   title = {Novel synergistic culturing approach for efficient and robust differentiation of human pluripotent stem cells into endothelial progenitors.},
   year = {2019}
}

TY  - CONF
ID  - 1599660
AU  - Farkaš, Šimon - Šimara, Pavel - Řeháková, Daniela - Souralová, Tereza - Koutná, Irena
PY  - 2019
TI  - Novel synergistic culturing approach for efficient and robust differentiation of human pluripotent stem cells into endothelial progenitors.
KW  - pluripotent stem cells
KW  - endothelial differentiation
N2  - Cellular therapy is a powerful tool for treating various human diseases and reparation of the damaged vasculature is a specialized subtype of this therapy. Using human pluripotent stem cells (hPSCs) as a starting material, it should be possible to generate high amounts of endothelial progenitor cells (EPCs). Our aim was to develop highly efficient, robust and easily reproducible differentiation protocol that would allow us to produce these high amounts of EPCs. Presented protocol follows proper physiological pathway by differentiating hPSCs in three phases through primitive streak and kinase insert domain receptor (KDR) positive mesoderm into EPCs. The differentiation protocol only takes 5 days and results in high amounts of pure EPCs. The robustness is demonstrated by using both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). Better efficiency and lesser line-to-line variability were observed for our differentiation protocol in comparison to other published protocols. Furthermore, derived EPCs expressed high proliferative potential EPC marker CD157 on their surface in addition to standard EPC markers CD31, CD144, CD34, KDR and C-X-C chemokine receptor type 4 (CXCR4).
ER  -

FARKAŠ, Šimon, Pavel ŠIMARA, Daniela ŘEHÁKOVÁ, Tereza SOURALOVÁ and Irena KOUTNÁ. Novel synergistic culturing approach for efficient and robust differentiation of human pluripotent stem cells into endothelial progenitors. In \textit{8. ročník konference Stem Cells and Cell Therapy: From Research to Modern Clinical Applications, Lednice, Czech Republic}. 2019.

Detailed Information on Publication Record