Transcriptomic Pattern of Genes Regulating Protein Response and
Status of Mitochondrial Activity Are Related to Oocyte
Maturational CompetenceA Transcriptomic Study

J 2019

Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational CompetenceA Transcriptomic Study

CHERMULA, Blazej, Maciej BRAZERT, Michal JEŠETA, Katarzyna OZEGOWSKA, Ievgenia KOCHEROVA et. al.

Basic information

Original name

Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational CompetenceA Transcriptomic Study

Authors

CHERMULA, Blazej (616 Poland), Maciej BRAZERT (616 Poland), Michal JEŠETA (203 Czech Republic, belonging to the institution), Katarzyna OZEGOWSKA (616 Poland), Ievgenia KOCHEROVA (616 Poland), Maurycy JANKOWSKI (616 Poland), Piotr CELICHOWSKI (616 Poland), Patrycja SUJKA-KORDOWSKA (616 Poland), Aneta KONWERSKA (616 Poland), Hanna PIOTROWSKA-KEMPISTY (616 Poland), Joanna BUDNA-TUKAN (616 Poland), Pawel ANTOSIK (616 Poland), Dorota BUKOWSKA (616 Poland), Marie MACHATKOVA (203 Czech Republic), Klaus P. BRUSSOW (616 Poland), Mariusz T. SKOWRONSKI (616 Poland), Leszek PAWELCZYK (616 Poland), Malgorzata BRUSKA (616 Poland), Michal NOWICKI (616 Poland) and Bartosz KEMPISTY (guarantor)

Edition

International Journal of Molecular Sciences, Basel, Switzerland, MDPI AG, 2019, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.556

RIV identification code

RIV/00216224:14110/19:00112138

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/ijms20092238

UT WoS

000469753500185

Keywords in English

pig; oocyte maturation; microarray; mitochondrial activity

Abstract

V originále

This paper aims to identify and describe new genetic markers involved in the processes of protein expression and modification reflected in the change of mitochondrial activity before and after in vitro maturation of the oocyte. Porcine oocytes collected from the ovaries of slaughtered landrace gilts were subjected to the process of in vitro maturation. Transcriptomic changes in the expression profile of oocyte genes involved in response to hypoxia, the transmembrane protein receptor serine threonine kinase signaling pathway, the transforming growth factor receptor signaling pathway, response to protein stimulus, and response to organic substance were investigated using microarrays. The expression values of these genes in oocytes was analyzed before (immature) and after (mature) in vitro maturation, with significant differences found. All the significantly altered genes showed downregulation after the maturation process. The most changed genes from these gene ontologies, FOS, ID2, VEGFA, BTG2, CYR61, ESR1, AR, TACR3, CCND2, CHRDL1, were chosen to be further validated, described and related to the literature. Additionally, the mitochondrial activity of the analyzed oocytes was measured using specific dyes. We found that the mitochondrial activity was higher before the maturation process. The analysis of these results and the available literature provides a novel insight on the processes that occur during in vitro oocyte maturation. While this knowledge may prove to be useful in further research of the procedures commonly associated with in vitro fertilization procedures, it serves mostly as a basic reference for further proteomic, in vivo, and clinical studies that are necessary to translate it into practical applications.

Citovat

CHERMULA, Blazej, Maciej BRAZERT, Michal JEŠETA, Katarzyna OZEGOWSKA, Ievgenia KOCHEROVA, Maurycy JANKOWSKI, Piotr CELICHOWSKI, Patrycja SUJKA-KORDOWSKA, Aneta KONWERSKA, Hanna PIOTROWSKA-KEMPISTY, Joanna BUDNA-TUKAN, Pawel ANTOSIK, Dorota BUKOWSKA, Marie MACHATKOVA, Klaus P. BRUSSOW, Mariusz T. SKOWRONSKI, Leszek PAWELCZYK, Malgorzata BRUSKA, Michal NOWICKI and Bartosz KEMPISTY. Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational CompetenceA Transcriptomic Study. International Journal of Molecular Sciences. Basel, Switzerland: MDPI AG, 2019, vol. 20, No 9, p. 1-19. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms20092238.

@article{1600436,
   author = {Chermula, Blazej and Brazert, Maciej and Ješeta, Michal and Ozegowska, Katarzyna and Kocherova, Ievgenia and Jankowski, Maurycy and Celichowski, Piotr and SujkaandKordowska, Patrycja and Konwerska, Aneta and PiotrowskaandKempisty, Hanna and BudnaandTukan, Joanna and Antosik, Pawel and Bukowska, Dorota and Machatkova, Marie and Brussow, Klaus P. and Skowronski, Mariusz T. and Pawelczyk, Leszek and Bruska, Malgorzata and Nowicki, Michal and Kempisty, Bartosz},
   article_location = {Basel, Switzerland},
   article_number = {9},
   doi = {http://dx.doi.org/10.3390/ijms20092238},
   keywords = {pig; oocyte maturation; microarray; mitochondrial activity},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational CompetenceA Transcriptomic Study},
   url = {http://dx.doi.org/10.3390/ijms20092238},
   volume = {20},
   year = {2019}
}

TY  - JOUR
ID  - 1600436
AU  - Chermula, Blazej - Brazert, Maciej - Ješeta, Michal - Ozegowska, Katarzyna - Kocherova, Ievgenia - Jankowski, Maurycy - Celichowski, Piotr - Sujka-Kordowska, Patrycja - Konwerska, Aneta - Piotrowska-Kempisty, Hanna - Budna-Tukan, Joanna - Antosik, Pawel - Bukowska, Dorota - Machatkova, Marie - Brussow, Klaus P. - Skowronski, Mariusz T. - Pawelczyk, Leszek - Bruska, Malgorzata - Nowicki, Michal - Kempisty, Bartosz
PY  - 2019
TI  - Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational CompetenceA Transcriptomic Study
JF  - International Journal of Molecular Sciences
VL  - 20
IS  - 9
SP  - 1-19
EP  - 1-19
PB  - MDPI AG
SN  - 14220067
KW  - pig
KW  - oocyte maturation
KW  - microarray
KW  - mitochondrial activity
UR  - http://dx.doi.org/10.3390/ijms20092238
L2  - http://dx.doi.org/10.3390/ijms20092238
N2  - This paper aims to identify and describe new genetic markers involved in the processes of protein expression and modification reflected in the change of mitochondrial activity before and after in vitro maturation of the oocyte. Porcine oocytes collected from the ovaries of slaughtered landrace gilts were subjected to the process of in vitro maturation. Transcriptomic changes in the expression profile of oocyte genes involved in response to hypoxia, the transmembrane protein receptor serine threonine kinase signaling pathway, the transforming growth factor receptor signaling pathway, response to protein stimulus, and response to organic substance were investigated using microarrays. The expression values of these genes in oocytes was analyzed before (immature) and after (mature) in vitro maturation, with significant differences found. All the significantly altered genes showed downregulation after the maturation process. The most changed genes from these gene ontologies, FOS, ID2, VEGFA, BTG2, CYR61, ESR1, AR, TACR3, CCND2, CHRDL1, were chosen to be further validated, described and related to the literature. Additionally, the mitochondrial activity of the analyzed oocytes was measured using specific dyes. We found that the mitochondrial activity was higher before the maturation process. The analysis of these results and the available literature provides a novel insight on the processes that occur during in vitro oocyte maturation. While this knowledge may prove to be useful in further research of the procedures commonly associated with in vitro fertilization procedures, it serves mostly as a basic reference for further proteomic, in vivo, and clinical studies that are necessary to translate it into practical applications.
ER  -

CHERMULA, Blazej, Maciej BRAZERT, Michal JEŠETA, Katarzyna OZEGOWSKA, Ievgenia KOCHEROVA, Maurycy JANKOWSKI, Piotr CELICHOWSKI, Patrycja SUJKA-KORDOWSKA, Aneta KONWERSKA, Hanna PIOTROWSKA-KEMPISTY, Joanna BUDNA-TUKAN, Pawel ANTOSIK, Dorota BUKOWSKA, Marie MACHATKOVA, Klaus P. BRUSSOW, Mariusz T. SKOWRONSKI, Leszek PAWELCZYK, Malgorzata BRUSKA, Michal NOWICKI and Bartosz KEMPISTY. Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational CompetenceA Transcriptomic Study. \textit{International Journal of Molecular Sciences}. Basel, Switzerland: MDPI AG, 2019, vol.~20, No~9, p.~1-19. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms20092238.

Detailed Information on Publication Record