Lectin PLL3, a Novel Monomeric Member of the Seven-Bladed beta-Propeller Lectin Family

FALTINEK, Lukáš, Eva FUJDIAROVÁ, Filip MELICHER, Josef HOUSER, Martina KAŠÁKOVÁ, Nikolay KONDAKOV, Leonid KONONOV, Kamil PARKAN, Sébastien VIDAL and Michaela WIMMEROVÁ. Lectin PLL3, a Novel Monomeric Member of the Seven-Bladed beta-Propeller Lectin Family. Molecules. Basel: MDPI, 2019, vol. 24, No 24, p. 1-20. ISSN 1420-3049. Available from: https://dx.doi.org/10.3390/molecules24244540.

Basic information

Original name

Lectin PLL3, a Novel Monomeric Member of the Seven-Bladed beta-Propeller Lectin Family

Name in Czech

Lektin PLL3, nový monomerní člen lektinové rodiny sedmi-lopatkových beta-vrtulí

Authors

FALTINEK, Lukáš (203 Czech Republic, belonging to the institution), Eva FUJDIAROVÁ (203 Czech Republic, belonging to the institution), Filip MELICHER (703 Slovakia, belonging to the institution), Josef HOUSER (203 Czech Republic, belonging to the institution), Martina KAŠÁKOVÁ (203 Czech Republic), Nikolay KONDAKOV (643 Russian Federation), Leonid KONONOV (643 Russian Federation), Kamil PARKAN (203 Czech Republic), Sébastien VIDAL (250 France) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Molecules, Basel, MDPI, 2019, 1420-3049

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Full Text

Impact factor

Impact factor: 3.267

RIV identification code

RIV/00216224:14310/19:00107959

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/molecules24244540

UT WoS

000507299600118

Keywords (in Czech)

Photorhabdus; lektin; O-methylované sacharidy; L-fukosa

Keywords in English

Photorhabdus; lectin; O-methylated saccharides; L-fucose

Tags

CF GEN, rivok

Tags

International impact, Reviewed

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Abstract

V originále

The Photorhabdus species is a Gram-negative bacteria of the family Morganellaceae that is known for its mutualistic relationship with Heterorhabditis nematodes and pathogenicity toward insects. This study is focused on the characterization of the recombinant lectin PLL3 with an origin in P. laumondii subsp. laumondii. PLL3 belongs to the PLL family of lectins with a seven-bladed beta-propeller fold. The binding properties of PLL3 were tested by hemagglutination assay, glycan array, isothermal titration calorimetry, and surface plasmon resonance, and its structure was determined by X-ray crystallography. Obtained data revealed that PLL3 binds similar carbohydrates to those that the other PLL family members bind, with some differences in the binding properties. PLL3 exhibited the highest affinity toward L-fucose and its derivatives but was also able to interact with O-methylated glycans and other ligands.

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Links

GA18-18964S, research and development project	Name: Lektiny a jejich úloha v interakci patogen/hostitel a buněčném rozpoznávání Investor: Czech Science Foundation
LQ1601, research and development project	Name: CEITEC 2020 (Acronym: CEITEC2020) Investor: Ministry of Education, Youth and Sports of the CR
LTC17076, research and development project	Name: Interdisciplinární přístup ke studiu biologických systémů na molekulární úrovni Investor: Ministry of Education, Youth and Sports of the CR, Interdisciplinary approach to study biological systems at the molecular level, INTER-COST
MUNI/A/1575/2018, interní kód MU	Name: Podpora biochemického výzkumu v roce 2019 Investor: Masaryk University, Category A
90043, large research infrastructures	Name: CIISB
90091, large research infrastructures	Name: NCMG