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@article{1600503,
author = {Lochmanová, Gabriela and Ihnatová, Ivana and Kuchaříková, Hana and Brabencová, Sylva and Zachová, Dagmar and Fajkus, Jiří and Zdráhal, Zbyněk and Fojtová, Miloslava},
article_number = {20},
doi = {http://dx.doi.org/10.3390/ijms20205093},
keywords = {Arabidopsis thaliana; epigenetics; histone; mass spectrometry; post-translational modifications; sodiumbutyrate; trichostatin A},
language = {eng},
issn = {1422-0067},
journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
title = {Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications},
url = {https://www.mdpi.com/1422-0067/20/20/5093},
volume = {20},
year = {2019}
}
TY - JOUR
ID - 1600503
AU - Lochmanová, Gabriela - Ihnatová, Ivana - Kuchaříková, Hana - Brabencová, Sylva - Zachová, Dagmar - Fajkus, Jiří - Zdráhal, Zbyněk - Fojtová, Miloslava
PY - 2019
TI - Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications
JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
VL - 20
IS - 20
SP - "5093"
EP - "5093"
SN - 14220067
KW - Arabidopsis thaliana
KW - epigenetics
KW - histone
KW - mass spectrometry
KW - post-translational modifications
KW - sodiumbutyrate
KW - trichostatin A
UR - https://www.mdpi.com/1422-0067/20/20/5093
L2 - https://www.mdpi.com/1422-0067/20/20/5093
N2 - A high degree of developmental plasticity enables plants to adapt to continuous, often unfavorable and unpredictable changes in their environment. At the molecular level, adaptive advantages for plants are primarily provided by epigenetic machinery including DNA methylation, histone modifications, and the activity of noncoding RNA molecules. Using a mass spectrometry-based proteomic approach, we examined the levels of acetylated histone peptide forms in Arabidopsis plants with a loss of function of histone deacetylase 6 (HDA6), and in plants germinated in the presence of HDA inhibitors trichostatin A (TSA) and sodium butyrate (NaB). Our analyses revealed particular lysine sites at histone sequences targeted by the HDA6 enzyme, and by TSA- and NaB-sensitive HDAs. Compared with plants exposed to drugs, more dramatic changes in the overall profiles of histone post-translational modifications were identified in hda6 mutants. However, loss of HDA6 was not sufficient by itself to induce hyperacetylation to the maximum degree, implying complementary activities of other HDAs. In contrast to hda6 mutants that did not exhibit any obvious phenotypic defects, the phenotypes of seedlings exposed to HDA inhibitors were markedly affected, showing that the effect of these drugs on early plant development is not limited to the modulation of histone acetylation levels.
ER -
LOCHMANOVÁ, Gabriela, Ivana IHNATOVÁ, Hana KUCHAŘÍKOVÁ, Sylva BRABENCOVÁ, Dagmar ZACHOVÁ, Jiří FAJKUS, Zbyněk ZDRÁHAL and Miloslava FOJTOVÁ. Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications. \textit{INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}. 2019, vol.~20, No~20, p.~''5093'', 14 pp. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms20205093.
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