Different Modes of Action of Genetic and Chemical
Downregulation of Histone Deacetylases with Respect to Plant
Development and Histone Modifications

J 2019

Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications

LOCHMANOVÁ, Gabriela, Ivana IHNATOVÁ, Hana KUCHAŘÍKOVÁ, Sylva BRABENCOVÁ, Dagmar ZACHOVÁ et. al.

Basic information

Original name

Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications

Authors

LOCHMANOVÁ, Gabriela (203 Czech Republic, belonging to the institution), Ivana IHNATOVÁ (703 Slovakia, belonging to the institution), Hana KUCHAŘÍKOVÁ (203 Czech Republic, belonging to the institution), Sylva BRABENCOVÁ (203 Czech Republic, belonging to the institution), Dagmar ZACHOVÁ (203 Czech Republic, belonging to the institution), Jiří FAJKUS (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution) and Miloslava FOJTOVÁ (203 Czech Republic, belonging to the institution)

Edition

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.556

RIV identification code

RIV/00216224:14740/19:00112144

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.3390/ijms20205093

UT WoS

000498822800125

Keywords in English

Arabidopsis thaliana; epigenetics; histone; mass spectrometry; post-translational modifications; sodiumbutyrate; trichostatin A

Abstract

V originále

A high degree of developmental plasticity enables plants to adapt to continuous, often unfavorable and unpredictable changes in their environment. At the molecular level, adaptive advantages for plants are primarily provided by epigenetic machinery including DNA methylation, histone modifications, and the activity of noncoding RNA molecules. Using a mass spectrometry-based proteomic approach, we examined the levels of acetylated histone peptide forms in Arabidopsis plants with a loss of function of histone deacetylase 6 (HDA6), and in plants germinated in the presence of HDA inhibitors trichostatin A (TSA) and sodium butyrate (NaB). Our analyses revealed particular lysine sites at histone sequences targeted by the HDA6 enzyme, and by TSA- and NaB-sensitive HDAs. Compared with plants exposed to drugs, more dramatic changes in the overall profiles of histone post-translational modifications were identified in hda6 mutants. However, loss of HDA6 was not sufficient by itself to induce hyperacetylation to the maximum degree, implying complementary activities of other HDAs. In contrast to hda6 mutants that did not exhibit any obvious phenotypic defects, the phenotypes of seedlings exposed to HDA inhibitors were markedly affected, showing that the effect of these drugs on early plant development is not limited to the modulation of histone acetylation levels.

Links

EF16_026/0008446, research and development project

Name: Integrace signálu a epigenetické reprogramování pro produktivitu rostlin

90043, large research infrastructures

Name: CIISB

Citovat

LOCHMANOVÁ, Gabriela, Ivana IHNATOVÁ, Hana KUCHAŘÍKOVÁ, Sylva BRABENCOVÁ, Dagmar ZACHOVÁ, Jiří FAJKUS, Zbyněk ZDRÁHAL and Miloslava FOJTOVÁ. Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2019, vol. 20, No 20, p. "5093", 14 pp. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms20205093.

@article{1600503,
   author = {Lochmanová, Gabriela and Ihnatová, Ivana and Kuchaříková, Hana and Brabencová, Sylva and Zachová, Dagmar and Fajkus, Jiří and Zdráhal, Zbyněk and Fojtová, Miloslava},
   article_number = {20},
   doi = {http://dx.doi.org/10.3390/ijms20205093},
   keywords = {Arabidopsis thaliana; epigenetics; histone; mass spectrometry; post-translational modifications; sodiumbutyrate; trichostatin A},
   language = {eng},
   issn = {1422-0067},
   journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
   title = {Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications},
   url = {https://www.mdpi.com/1422-0067/20/20/5093},
   volume = {20},
   year = {2019}
}

TY  - JOUR
ID  - 1600503
AU  - Lochmanová, Gabriela - Ihnatová, Ivana - Kuchaříková, Hana - Brabencová, Sylva - Zachová, Dagmar - Fajkus, Jiří - Zdráhal, Zbyněk - Fojtová, Miloslava
PY  - 2019
TI  - Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications
JF  - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
VL  - 20
IS  - 20
SP  - "5093"
EP  - "5093"
SN  - 14220067
KW  - Arabidopsis thaliana
KW  - epigenetics
KW  - histone
KW  - mass spectrometry
KW  - post-translational modifications
KW  - sodiumbutyrate
KW  - trichostatin A
UR  - https://www.mdpi.com/1422-0067/20/20/5093
L2  - https://www.mdpi.com/1422-0067/20/20/5093
N2  - A high degree of developmental plasticity enables plants to adapt to continuous, often unfavorable and unpredictable changes in their environment. At the molecular level, adaptive advantages for plants are primarily provided by epigenetic machinery including DNA methylation, histone modifications, and the activity of noncoding RNA molecules. Using a mass spectrometry-based proteomic approach, we examined the levels of acetylated histone peptide forms in Arabidopsis plants with a loss of function of histone deacetylase 6 (HDA6), and in plants germinated in the presence of HDA inhibitors trichostatin A (TSA) and sodium butyrate (NaB). Our analyses revealed particular lysine sites at histone sequences targeted by the HDA6 enzyme, and by TSA- and NaB-sensitive HDAs. Compared with plants exposed to drugs, more dramatic changes in the overall profiles of histone post-translational modifications were identified in hda6 mutants. However, loss of HDA6 was not sufficient by itself to induce hyperacetylation to the maximum degree, implying complementary activities of other HDAs. In contrast to hda6 mutants that did not exhibit any obvious phenotypic defects, the phenotypes of seedlings exposed to HDA inhibitors were markedly affected, showing that the effect of these drugs on early plant development is not limited to the modulation of histone acetylation levels.
ER  -

LOCHMANOVÁ, Gabriela, Ivana IHNATOVÁ, Hana KUCHAŘÍKOVÁ, Sylva BRABENCOVÁ, Dagmar ZACHOVÁ, Jiří FAJKUS, Zbyněk ZDRÁHAL and Miloslava FOJTOVÁ. Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications. \textit{INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}. 2019, vol.~20, No~20, p.~''5093'', 14 pp. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms20205093.

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