Detailed Information on Publication Record
2019
DNA Damage Changes Distribution Pattern and Levels of HP1 Protein Isoforms in the Nucleolus and Increases Phosphorylation of HP1 beta-Ser88
LEGARTOVÁ, Sofia, Gabriela LOCHMANOVÁ, Zbyněk ZDRÁHAL, Stanislav KOZUBEK, Jiří ŠPONER et. al.Basic information
Original name
DNA Damage Changes Distribution Pattern and Levels of HP1 Protein Isoforms in the Nucleolus and Increases Phosphorylation of HP1 beta-Ser88
Authors
LEGARTOVÁ, Sofia, Gabriela LOCHMANOVÁ (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution), Stanislav KOZUBEK (203 Czech Republic), Jiří ŠPONER (203 Czech Republic), Miroslav KREPL (203 Czech Republic), Pavlína POKORNÁ (203 Czech Republic) and Eva BÁRTOVÁ (203 Czech Republic)
Edition
CELLS, 2019, 2073-4409
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10601 Cell biology
Country of publisher
Switzerland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 4.366
RIV identification code
RIV/00216224:14740/19:00107962
Organization unit
Central European Institute of Technology
UT WoS
000489103800156
Keywords in English
HP1; irradiation; nucleolus; phosphorylation; mass spectrometry; epigenetics; FLIM-FRET
Tags
International impact, Reviewed
Změněno: 9/10/2024 14:11, Ing. Martina Blahová
Abstract
V originále
The family of heterochromatin protein 1 (HP1) isoforms is essential for chromatin packaging, regulation of gene expression, and repair of damaged DNA. Here we document that gamma-radiation reduced the number of HP1 alpha-positive foci, but not HP1 beta and HP1 gamma foci, located in the vicinity of the fibrillarin-positive region of the nucleolus. The additional analysis confirmed that gamma-radiation has the ability to significantly decrease the level of HP1 alpha in rDNA promoter and rDNA encoding 28S rRNA. By mass spectrometry, we showed that treatment by gamma-rays enhanced the HP1 beta serine 88 phosphorylation (S88ph), but other analyzed modifications of HP1 beta, including S161ph/Y163ph, S171ph, and S174ph, were not changed in cells exposed to gamma-rays or treated by the HDAC inhibitor (HDACi). Interestingly, a combination of HDACi and gamma-radiation increased the level of HP1 alpha and HP1 gamma. The level of HP1 beta remained identical before and after the HDACi/gamma-rays treatment, but HDACi strengthened HP1 beta interaction with the KRAB-associated protein 1 (KAP1) protein. Conversely, HP1 gamma did not interact with KAP1, although approximately 40% of HP1 gamma foci co-localized with accumulated KAP1. Especially HP1 gamma foci at the periphery of nucleoli were mostly absent of KAP1. Together, DNA damage changed the morphology, levels, and interaction properties of HP1 isoforms. Also, gamma-irradiation-induced hyperphosphorylation of the HP1 beta protein; thus, HP1 beta-S88ph could be considered as an important marker of DNA damage.
Links
GA18-07384S, research and development project |
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LM2015043, research and development project |
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90043, large research infrastructures |
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