| TRNĚNÝ, Oldřich, David VLK, Eliška MACKOVÁ, Michaela MATOUŠKOVÁ, Jana ŘEPKOVÁ, Jan NEDĚLNÍK, Jan HOFBAUER, Karel VEJRAŽKA, Hana JAKEŠOVÁ, Jan JANSA, Lubomír PIÁLEK and Daniela KNOTOVÁ. Allelic variants for candidate nitrogen fixation genes revealed by sequencing in red clover (Trifolium pratense L.). International Journal of Molecular Sciences. 2019, vol. 20, No 21, p. 1-26. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms20215470. |
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@article{1600971,
author = {Trněný, Oldřich and Vlk, David and Macková, Eliška and Matoušková, Michaela and Řepková, Jana and Nedělník, Jan and Hofbauer, Jan and Vejražka, Karel and Jakešová, Hana and Jansa, Jan and Piálek, Lubomír and Knotová, Daniela},
article_number = {21},
doi = {http://dx.doi.org/10.3390/ijms20215470},
keywords = {associated genes; associated polymorphisms; genome-wide association; biological nitrogen fixation; red clover},
language = {eng},
issn = {1422-0067},
journal = {International Journal of Molecular Sciences},
title = {Allelic variants for candidate nitrogen fixation genes revealed by sequencing in red clover (Trifolium pratense L.)},
url = {http://dx.doi.org/10.3390/ijms20215470},
volume = {20},
year = {2019}
}
TY - JOUR
ID - 1600971
AU - Trněný, Oldřich - Vlk, David - Macková, Eliška - Matoušková, Michaela - Řepková, Jana - Nedělník, Jan - Hofbauer, Jan - Vejražka, Karel - Jakešová, Hana - Jansa, Jan - Piálek, Lubomír - Knotová, Daniela
PY - 2019
TI - Allelic variants for candidate nitrogen fixation genes revealed by sequencing in red clover (Trifolium pratense L.)
JF - International Journal of Molecular Sciences
VL - 20
IS - 21
SP - 1-26
EP - 1-26
SN - 14220067
KW - associated genes
KW - associated polymorphisms
KW - genome-wide association
KW - biological nitrogen fixation
KW - red clover
UR - http://dx.doi.org/10.3390/ijms20215470
L2 - http://dx.doi.org/10.3390/ijms20215470
N2 - Plant–rhizobia symbiosis can activate key genes involved in regulating nodulation associated with biological nitrogen fixation (BNF). Although the general molecular basis of the BNF process is frequently studied, little is known about its intraspecific variability and the characteristics of its allelic variants. This study’s main goals were to describe phenotypic and genotypic variation in the context of nitrogen fixation in red clover (Trifolium pratense L.) and identify variants in BNF candidate genes associated with BNF efficiency. Acetylene reduction assay validation was the criterion for selecting individual plants with particular BNF rates. Sequences in 86 key candidate genes were obtained by hybridization-based sequence capture target enrichment of plants with alternative phenotypes for nitrogen fixation. Two genes associated with BNF were identified: ethylene response factor required for nodule differentiation (EFD) and molybdate transporter 1 (MOT1). In addition, whole-genome population genotyping by double-digest restriction-site-associated sequencing (ddRADseq) was performed, and BNF was evaluated by the natural 15N abundance method. Polymorphisms associated with BNF and reflecting phenotype variability were identified. The genetic structure of plant accessions was not linked to BNF rate of measured plants. Knowledge of the genetic variation within BNF candidate genes and the characteristics of genetic variants will be beneficial in molecular diagnostics and breeding of red clover.
ER -
TRNĚNÝ, Oldřich, David VLK, Eliška MACKOVÁ, Michaela MATOUŠKOVÁ, Jana ŘEPKOVÁ, Jan NEDĚLNÍK, Jan HOFBAUER, Karel VEJRAŽKA, Hana JAKEŠOVÁ, Jan JANSA, Lubomír PIÁLEK and Daniela KNOTOVÁ. Allelic variants for candidate nitrogen fixation genes revealed by sequencing in red clover (Trifolium pratense L.). \textit{International Journal of Molecular Sciences}. 2019, vol.~20, No~21, p.~1-26. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms20215470.
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