Production and characterization of galactose-specific lectin PluLec from plant pathogen Photorhabdus luminescens

KORSÁK, Marek, Josef HOUSER, Eva FUJDIAROVÁ, Marie POKORNÁ, Jan KOMÁREK and Michaela WIMMEROVÁ. Production and characterization of galactose-specific lectin PluLec from plant pathogen Photorhabdus luminescens. In XX. setkání biochemiků a molekulárních biologů, 2019. 2019. ISBN 978-80-210-9420-8.

Basic information

• Original name: Production and characterization of galactose-specific lectin PluLec from plant pathogen Photorhabdus luminescens
• Authors: KORSÁK, Marek (703 Slovakia, belonging to the institution), Josef HOUSER (203 Czech Republic, belonging to the institution), Eva FUJDIAROVÁ (203 Czech Republic, belonging to the institution), Marie POKORNÁ (203 Czech Republic, belonging to the institution), Jan KOMÁREK (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: XX. setkání biochemiků a molekulárních biologů, 2019. 2019.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/19:00112191
• Organization unit: Faculty of Science
• ISBN: 978-80-210-9420-8
• Keywords (in Czech): Photorhabdus luminescens;lektin
• Keywords in English: Photorhabdus luminescens;lectin

Abstract

Lectins are a group of proteins of non-immune origin that can reversibly and specifically interact with carbohydrates. They are found in all known living organisms and are involved in recognition events in various physiological and pathological processes like intercellular communication, adhesion, migration and host-pathogen interactions. Lectins are commonly used for characterization of carbohydrate structures, for purification of glycoproteins and to specifically label the cell surface structures. They are also used for cell separation and typing. Photorhabdus luminescens is a naturally bioluminescent Gram-negative bacterium which symbiotically lives in Heterorhabditidae nematodes and is strongly pathogenic to insect larvae. PluLec, a putative lectin from Photorhabdus luminescens that we discovered, is a homologue of PA-IL lectin, which is D galactose specific, Ca2+ dependent, cytotoxic lectin from opportunistic pathogen Pseudomonas aeruginosa, involved in facilitating infection in patients with compromised immunity. This research is focused on cloning, production and structural-functional characterization of recombinant protein PluLec using various methods, namely dynamic light scattering, differential scanning fluorimetry, isothermal titration calorimetry, hemagglutination, glycan array, surface plasmon resonance, analytical ultracentrifugation and protein X-ray crystallography. Detailed knowledge of the structure and function of PluLec can help to understand the mechanism of P. luminescens infection.

Links

• MUNI/A/1575/2018, interní kód MU: Name: Podpora biochemického výzkumu v roce 2019

Investor: Masaryk University, Category A