Effect of cysteine peptidase inhibitor of Eudiplozoon
nipponicum (Monogenea) on cytokine expression of macrophages in
vitro

J 2020

Effect of cysteine peptidase inhibitor of Eudiplozoon nipponicum (Monogenea) on cytokine expression of macrophages in vitro

ILGOVÁ, Jana, Lenka KAVANOVÁ, Katarína MATIAŠKOVÁ, Jiří SALÁT, Martin KAŠNÝ et. al.

Basic information

Original name

Effect of cysteine peptidase inhibitor of Eudiplozoon nipponicum (Monogenea) on cytokine expression of macrophages in vitro

Authors

ILGOVÁ, Jana (703 Slovakia, guarantor, belonging to the institution), Lenka KAVANOVÁ (203 Czech Republic), Katarína MATIAŠKOVÁ (703 Slovakia), Jiří SALÁT (203 Czech Republic) and Martin KAŠNÝ (203 Czech Republic, belonging to the institution)

Edition

Molecular and Biochemical Parasitology, Amsterdam, Elsevier, 2020, 0166-6851

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL URL

Impact factor

Impact factor: 1.759

RIV identification code

RIV/00216224:14310/20:00114021

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.molbiopara.2019.111248

UT WoS

000510977300003

Keywords in English

Monogenea; Cystatin; Cytokines; Immunomodulation; IL-10; TNF-alpha

Abstract

V originále

The gills of the common carp, whose mucosal surface belongs to the key defence mechanisms of piscine immunity, can be infested with both the larval and adult stage of Eudiplozoon nipponicum (Monogenea). Although on their own, monogeneans do not considerably compromise their hosts’ health status, fish with epithelial barriers damaged in parasite feeding and attachment sites are at an increased risk of bacterial challenge with possible harmful consequences. Several studies suggest that helminth parasites of teleost fish evade and manipulate host immune system via their excretory-secretory products, but our knowledge of these processes in the monogeneans is limited. Cysteine peptidase inhibitors (CPI), which are found in the secretions of numerous parasites, often induce immunosuppression by subverting Th1 mechanisms and drawing the immune system towards a Th2/Treg response. We employed the qPCR to test the effect of recently characterised CPI of E. nipponicum (rEnStef) on the mRNA expression of pro-inflammatory cytokine TNF-alpha and anti-inflammatory cytokine IL-10 produced by porcine macrophages in vitro. After an initial preincubation with rEnStef, we stimulated the macrophages using LPS. By inducing a Th1 pro-inflammatory response, we imitated the immune reaction during a bacterial challenge in tissue damaged by the feeding and attachment of E. nipponicum. We observed a significant dose-dependent downregulation of the expression of TNF-aplha and IL-10 cytokines. The observed suppression of TNF-alpha expression by rEnStef could result in decreased pathogen control, which might in turn lead to increased rates of secondary bacterial infections in fish infected by E. nipponicum.

Links

GAP506/12/1258, research and development project

Name: Interakce hostitel-parazit u krevsajících diplozoidních monogeneí: Výzkum vysoce specializovaných adaptací k parazitismu

Investor: Czech Science Foundation

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

Citovat

ILGOVÁ, Jana, Lenka KAVANOVÁ, Katarína MATIAŠKOVÁ, Jiří SALÁT and Martin KAŠNÝ. Effect of cysteine peptidase inhibitor of Eudiplozoon nipponicum (Monogenea) on cytokine expression of macrophages in vitro. Molecular and Biochemical Parasitology. Amsterdam: Elsevier, 2020, vol. 235, JAN 2020, p. 1-4. ISSN 0166-6851. Available from: https://dx.doi.org/10.1016/j.molbiopara.2019.111248.

@article{1601610,
   author = {Ilgová, Jana and Kavanová, Lenka and Matiašková, Katarína and Salát, Jiří and Kašný, Martin},
   article_location = {Amsterdam},
   article_number = {JAN 2020},
   doi = {http://dx.doi.org/10.1016/j.molbiopara.2019.111248},
   keywords = {Monogenea; Cystatin; Cytokines; Immunomodulation; IL-10; TNF-alpha},
   language = {eng},
   issn = {0166-6851},
   journal = {Molecular and Biochemical Parasitology},
   title = {Effect of cysteine peptidase inhibitor of Eudiplozoon nipponicum (Monogenea) on cytokine expression of macrophages in vitro},
   url = {https://www.sciencedirect.com/science/article/pii/S016668511930163X?via%3Dihub},
   volume = {235},
   year = {2020}
}

TY  - JOUR
ID  - 1601610
AU  - Ilgová, Jana - Kavanová, Lenka - Matiašková, Katarína - Salát, Jiří - Kašný, Martin
PY  - 2020
TI  - Effect of cysteine peptidase inhibitor of Eudiplozoon nipponicum (Monogenea) on cytokine expression of macrophages in vitro
JF  - Molecular and Biochemical Parasitology
VL  - 235
IS  - JAN 2020
SP  - 1-4
EP  - 1-4
PB  - Elsevier
SN  - 01666851
KW  - Monogenea
KW  - Cystatin
KW  - Cytokines
KW  - Immunomodulation
KW  - IL-10
KW  - TNF-alpha
UR  - https://www.sciencedirect.com/science/article/pii/S016668511930163X?via%3Dihub
N2  - The gills of the common carp, whose mucosal surface belongs to the key defence mechanisms of piscine immunity, can be infested with both the larval and adult stage of Eudiplozoon nipponicum (Monogenea). Although on their own, monogeneans do not considerably compromise their hosts’ health status, fish with epithelial barriers damaged in parasite feeding and attachment sites are at an increased risk of bacterial challenge with possible harmful consequences. Several studies suggest that helminth parasites of teleost fish evade and manipulate host immune system via their excretory-secretory products, but our knowledge of these processes in the monogeneans is limited. Cysteine peptidase inhibitors (CPI), which are found in the secretions of numerous parasites, often induce immunosuppression by subverting Th1 mechanisms and drawing the immune system towards a Th2/Treg response. We employed the qPCR to test the effect of recently characterised CPI of E. nipponicum (rEnStef) on the mRNA expression of pro-inflammatory cytokine TNF-alpha and anti-inflammatory cytokine IL-10 produced by porcine macrophages in vitro. After an initial preincubation with rEnStef, we stimulated the macrophages using LPS. By inducing a Th1 pro-inflammatory response, we imitated the immune reaction during a bacterial challenge in tissue damaged by the feeding and attachment of E. nipponicum. We observed a significant dose-dependent downregulation of the expression of TNF-aplha and IL-10 cytokines. The observed suppression of TNF-alpha expression by rEnStef could result in decreased pathogen control, which might in turn lead to increased rates of secondary bacterial infections in fish infected by E. nipponicum.
ER  -

ILGOVÁ, Jana, Lenka KAVANOVÁ, Katarína MATIAŠKOVÁ, Jiří SALÁT and Martin KAŠNÝ. Effect of cysteine peptidase inhibitor of Eudiplozoon nipponicum (Monogenea) on cytokine expression of macrophages in vitro. \textit{Molecular and Biochemical Parasitology}. Amsterdam: Elsevier, 2020, vol.~235, JAN 2020, p.~1-4. ISSN~0166-6851. Available from: https://dx.doi.org/10.1016/j.molbiopara.2019.111248.

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