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@article{1603838,
author = {Vrbová, Eliška and Grillová, Linda and Mikalová, Lenka and Pospíšilová, Petra and Strnadel, Radim and Dastychová, Eliška and Kojanova, Martina and Kreidlova, Miluse and Vanousova, Daniela and Rob, Filip and Prochazka, Premysl and Krchňáková, Alena and Vašků, Vladimír and Woznicová, Vladana and Dvořáková Heroldová, Monika and Kuklova, Ivana and Zakoucka, Hana and Šmajs, David},
article_location = {San Francisco},
article_number = {5},
doi = {http://dx.doi.org/10.1371/journal.pone.0217611},
keywords = {SYPHILIS-CAUSING STRAINS; MACROLIDE RESISTANCE; IDENTIFIED GENOTYPES; PREVALENCE; GENE; MUTATIONS; EPIDEMIOLOGY; SURVEILLANCE; ASSOCIATION; PHYLOGENIES},
language = {eng},
issn = {1932-6203},
journal = {Plos one},
title = {MLST typing of Treponema pallidum subsp. pallidum in the Czech Republic during 2004-2017: Clinical isolates belonged to 25 allelic profiles and harbored 8 novel allelic variants},
url = {http://dx.doi.org/10.1371/journal.pone.0217611},
volume = {14},
year = {2019}
}
TY - JOUR
ID - 1603838
AU - Vrbová, Eliška - Grillová, Linda - Mikalová, Lenka - Pospíšilová, Petra - Strnadel, Radim - Dastychová, Eliška - Kojanova, Martina - Kreidlova, Miluse - Vanousova, Daniela - Rob, Filip - Prochazka, Premysl - Krchňáková, Alena - Vašků, Vladimír - Woznicová, Vladana - Dvořáková Heroldová, Monika - Kuklova, Ivana - Zakoucka, Hana - Šmajs, David
PY - 2019
TI - MLST typing of Treponema pallidum subsp. pallidum in the Czech Republic during 2004-2017: Clinical isolates belonged to 25 allelic profiles and harbored 8 novel allelic variants
JF - Plos one
VL - 14
IS - 5
SP - 1-15
EP - 1-15
PB - Public Library of Science
SN - 19326203
KW - SYPHILIS-CAUSING STRAINS
KW - MACROLIDE RESISTANCE
KW - IDENTIFIED GENOTYPES
KW - PREVALENCE
KW - GENE
KW - MUTATIONS
KW - EPIDEMIOLOGY
KW - SURVEILLANCE
KW - ASSOCIATION
KW - PHYLOGENIES
UR - http://dx.doi.org/10.1371/journal.pone.0217611
L2 - http://dx.doi.org/10.1371/journal.pone.0217611
N2 - A recently introduced Multilocus Sequence Typing scheme for Treponema pallidum subsp. pallidum was applied to clinical samples collected from 2004 to 2017 from the two largest cities (Prague and Brno) in the Czech Republic. Altogether, a total of 675 samples were tested in this study and 281 of them were found PCR-positive for treponemal DNA and typeable. Most of the typed samples (n = 281) were swabs from primary or secondary syphilis lesions (n = 231), and only a minority were whole blood or tissue samples (n = 50). Swab samples from patients with rapid plasma regain (RPR) values of 1-1024 were more frequently PCR-positive (84.6%) compared to samples from patients with non-reactive RPR test (46.5%; p-value = 0.0001). Out of 281 typeable samples, 136 were fully-typed at all TP0136, TP0548, and TP0705 loci. Among the fully and partially typed samples, 25 different allelic profiles were identified. Altogether, eight novel allelic variants were found among fully (n = 5) and partially (n = 3) typed samples. The distribution of TPA allelic profiles identified in the Czech Republic from 2004 to 2017 revealed a dynamic character with allelic profiles disappearing and emerging over time. While the number of samples with the A2058G mutation was seen to increase (86.7% in 2016/2017), the number of samples harboring the A2059G mutation was found to have decreased over time (3.3% in 2016/2017). In addition, we found several allelic profile associations with macrolide resistance or susceptibility, the gender of patients, as well as patient residence.
ER -
VRBOVÁ, Eliška, Linda GRILLOVÁ, Lenka MIKALOVÁ, Petra POSPÍŠILOVÁ, Radim STRNADEL, Eliška DASTYCHOVÁ, Martina KOJANOVA, Miluse KREIDLOVA, Daniela VANOUSOVA, Filip ROB, Premysl PROCHAZKA, Alena KRCHŇÁKOVÁ, Vladimír VAŠKŮ, Vladana WOZNICOVÁ, Monika DVOŘÁKOVÁ HEROLDOVÁ, Ivana KUKLOVA, Hana ZAKOUCKA and David ŠMAJS. MLST typing of Treponema pallidum subsp. pallidum in the Czech Republic during 2004-2017: Clinical isolates belonged to 25 allelic profiles and harbored 8 novel allelic variants. \textit{Plos one}. San Francisco: Public Library of Science, 2019, vol.~14, No~5, p.~1-15. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0217611.
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