Porcine pathogenic Escherichia coli strains differ from human
fecal strains in occurrence of bacteriocin types

J 2019

Porcine pathogenic Escherichia coli strains differ from human fecal strains in occurrence of bacteriocin types

BOSÁK, Juraj; Matěj HRALA; Viktória PIRKOVÁ; Lenka MICENKOVÁ; Alois CIZEK et. al.

Základní údaje

Originální název

Porcine pathogenic Escherichia coli strains differ from human fecal strains in occurrence of bacteriocin types

Autoři

BOSÁK, Juraj; Matěj HRALA; Viktória PIRKOVÁ; Lenka MICENKOVÁ; Alois CIZEK; Jiri SMOLA; Dana KUCEROVA; Zdenka VACKOVA; Eva BUDINSKÁ; Ivana KOLÁČKOVÁ a David ŠMAJS

Vydání

Veterinary Microbiology, AMSTERDAM, Elsevier, 2019, 0378-1135

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.030

Kód RIV

RIV/00216224:14110/19:00108018

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1016/j.vetmic.2019.04.003

UT WoS

000468718900017

EID Scopus

2-s2.0-85064274613

Klíčová slova anglicky

Bacteriocin; Colicin; E. coli; STEC; ETEC; Pig

Štítky

14110513, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 10. 2022 13:12, Mgr. Tereza Miškechová

Anotace

V originále

Enterotoxigenic and Shiga-toxigenic Escherichia coli (i.e., ETEC and STEC) are important causative agents of human and animal diseases. In humans, infections range from mild diarrhea to severe life-threating conditions, while infections of piglets result in lower weight gain and higher pig mortality with the accompanying significant economic losses. In this study, frequencies of four phylogenetic groups, fourteen virulence- and thirty bacteriocin determinants were analyzed in a set of 443 fecal E. coli isolates from diseased pigs and compared to a previously characterized set of 1283 human fecal E. coli isolates collected in the same geographical region. In addition, these characteristics were compared among ETEC, STEC, and non-toxigenic porcine E. coli isolates. Phylogenetic group A was prevalent among porcine pathogenic E. coli isolates, whereas the frequency of phylogroup B2, adhesion/invasion (firnA, pap, sfa, afaI, ial, ipaH, and pCVD432) and iron acquisition (aer and iucC) determinants were less frequent compared to human fecal isolates. Additionally, porcine isolates differed from human isolates relative to the spectrum of produced bacteriocins. While human fecal isolates encoded colicins and microcins with a similar prevalence, porcine pathogenic E. coli isolates produced predominantly colicins (94% of isolates); especially colicins B (42.6%), M (40.1%), and Ib (34.0%), which are encoded on large conjugative plasmids. The observed high prevalence of these colicin determinants suggests the importance of large colicinogenic plasmids and/or the importance of colicin production in intestinal inflammatory conditions.

Návaznosti

EF16_013/0001761, projekt VaV

Název: RECETOX RI

GA16-21649S, projekt VaV

Název: Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

Investor: Grantová agentura ČR, Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

LM2015051, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

MUNI/A/1087/2018, interní kód MU

Název: Molekulární a buněčná biologie pro biomedicínské vědy

Investor: Masarykova univerzita, Molekulární a buněčná biologie pro biomedicínské vědy, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

BOSÁK, Juraj; Matěj HRALA; Viktória PIRKOVÁ; Lenka MICENKOVÁ; Alois CIZEK; Jiri SMOLA; Dana KUCEROVA; Zdenka VACKOVA; Eva BUDINSKÁ; Ivana KOLÁČKOVÁ a David ŠMAJS. Porcine pathogenic Escherichia coli strains differ from human fecal strains in occurrence of bacteriocin types. Veterinary Microbiology. AMSTERDAM: Elsevier, 2019, roč. 232, MAY 2019, s. 121-127. ISSN 0378-1135. Dostupné z: https://doi.org/10.1016/j.vetmic.2019.04.003.

@article{1603839,
   author = {Bosák, Juraj and Hrala, Matěj and Pirková, Viktória and Micenková, Lenka and Cizek, Alois and Smola, Jiri and Kucerova, Dana and Vackova, Zdenka and Budinská, Eva and Koláčková, Ivana and Šmajs, David},
   article_location = {AMSTERDAM},
   article_number = {MAY 2019},
   doi = {https://doi.org/10.1016/j.vetmic.2019.04.003},
   keywords = {Bacteriocin; Colicin; E. coli; STEC; ETEC; Pig},
   language = {eng},
   issn = {0378-1135},
   journal = {Veterinary Microbiology},
   title = {Porcine pathogenic Escherichia coli strains differ from human fecal strains in occurrence of bacteriocin types},
   url = {http://dx.doi.org/10.1016/j.vetmic.2019.04.003},
   volume = {232},
   year = {2019}
}

TY  - JOUR
ID  - 1603839
AU  - Bosák, Juraj - Hrala, Matěj - Pirková, Viktória - Micenková, Lenka - Cizek, Alois - Smola, Jiri - Kucerova, Dana - Vackova, Zdenka - Budinská, Eva - Koláčková, Ivana - Šmajs, David
PY  - 2019
TI  - Porcine pathogenic Escherichia coli strains differ from human fecal strains in occurrence of bacteriocin types
JF  - Veterinary Microbiology
VL  - 232
IS  - MAY 2019
SP  - 121-127
EP  - 121-127
PB  - Elsevier
SN  - 03781135
KW  - Bacteriocin
KW  - Colicin
KW  - E. coli
KW  - STEC
KW  - ETEC
KW  - Pig
UR  - http://dx.doi.org/10.1016/j.vetmic.2019.04.003
L2  - http://dx.doi.org/10.1016/j.vetmic.2019.04.003
N2  - Enterotoxigenic and Shiga-toxigenic Escherichia coli (i.e., ETEC and STEC) are important causative agents of human and animal diseases. In humans, infections range from mild diarrhea to severe life-threating conditions, while infections of piglets result in lower weight gain and higher pig mortality with the accompanying significant economic losses. In this study, frequencies of four phylogenetic groups, fourteen virulence- and thirty bacteriocin determinants were analyzed in a set of 443 fecal E. coli isolates from diseased pigs and compared to a previously characterized set of 1283 human fecal E. coli isolates collected in the same geographical region. In addition, these characteristics were compared among ETEC, STEC, and non-toxigenic porcine E. coli isolates. Phylogenetic group A was prevalent among porcine pathogenic E. coli isolates, whereas the frequency of phylogroup B2, adhesion/invasion (firnA, pap, sfa, afaI, ial, ipaH, and pCVD432) and iron acquisition (aer and iucC) determinants were less frequent compared to human fecal isolates. Additionally, porcine isolates differed from human isolates relative to the spectrum of produced bacteriocins. While human fecal isolates encoded colicins and microcins with a similar prevalence, porcine pathogenic E. coli isolates produced predominantly colicins (94% of isolates); especially colicins B (42.6%), M (40.1%), and Ib (34.0%), which are encoded on large conjugative plasmids. The observed high prevalence of these colicin determinants suggests the importance of large colicinogenic plasmids and/or the importance of colicin production in intestinal inflammatory conditions.
ER  -

BOSÁK, Juraj; Matěj HRALA; Viktória PIRKOVÁ; Lenka MICENKOVÁ; Alois CIZEK; Jiri SMOLA; Dana KUCEROVA; Zdenka VACKOVA; Eva BUDINSKÁ; Ivana KOLÁČKOVÁ a David ŠMAJS. Porcine pathogenic Escherichia coli strains differ from human fecal strains in occurrence of bacteriocin types. \textit{Veterinary Microbiology}. AMSTERDAM: Elsevier, 2019, roč.~232, MAY 2019, s.~121-127. ISSN~0378-1135. Dostupné z: https://doi.org/10.1016/j.vetmic.2019.04.003.

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