Porcine pathogenic Escherichia coli strains differ from human
fecal strains in occurrence of bacteriocin types

J 2019

Porcine pathogenic Escherichia coli strains differ from human fecal strains in occurrence of bacteriocin types

BOSÁK, Juraj, Matěj HRALA, Viktória PIRKOVÁ, Lenka MICENKOVÁ, Alois CIZEK et. al.

Basic information

Original name

Porcine pathogenic Escherichia coli strains differ from human fecal strains in occurrence of bacteriocin types

Authors

BOSÁK, Juraj (703 Slovakia, belonging to the institution), Matěj HRALA (203 Czech Republic, belonging to the institution), Viktória PIRKOVÁ (703 Slovakia, belonging to the institution), Lenka MICENKOVÁ (703 Slovakia, belonging to the institution), Alois CIZEK (203 Czech Republic), Jiri SMOLA (203 Czech Republic), Dana KUCEROVA (203 Czech Republic), Zdenka VACKOVA (203 Czech Republic), Eva BUDINSKÁ (703 Slovakia, belonging to the institution), Ivana KOLÁČKOVÁ (203 Czech Republic) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

Veterinary Microbiology, AMSTERDAM, Elsevier, 2019, 0378-1135

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.030

RIV identification code

RIV/00216224:14110/19:00108018

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.vetmic.2019.04.003

UT WoS

000468718900017

Keywords in English

Bacteriocin; Colicin; E. coli; STEC; ETEC; Pig

Abstract

V originále

Enterotoxigenic and Shiga-toxigenic Escherichia coli (i.e., ETEC and STEC) are important causative agents of human and animal diseases. In humans, infections range from mild diarrhea to severe life-threating conditions, while infections of piglets result in lower weight gain and higher pig mortality with the accompanying significant economic losses. In this study, frequencies of four phylogenetic groups, fourteen virulence- and thirty bacteriocin determinants were analyzed in a set of 443 fecal E. coli isolates from diseased pigs and compared to a previously characterized set of 1283 human fecal E. coli isolates collected in the same geographical region. In addition, these characteristics were compared among ETEC, STEC, and non-toxigenic porcine E. coli isolates. Phylogenetic group A was prevalent among porcine pathogenic E. coli isolates, whereas the frequency of phylogroup B2, adhesion/invasion (firnA, pap, sfa, afaI, ial, ipaH, and pCVD432) and iron acquisition (aer and iucC) determinants were less frequent compared to human fecal isolates. Additionally, porcine isolates differed from human isolates relative to the spectrum of produced bacteriocins. While human fecal isolates encoded colicins and microcins with a similar prevalence, porcine pathogenic E. coli isolates produced predominantly colicins (94% of isolates); especially colicins B (42.6%), M (40.1%), and Ib (34.0%), which are encoded on large conjugative plasmids. The observed high prevalence of these colicin determinants suggests the importance of large colicinogenic plasmids and/or the importance of colicin production in intestinal inflammatory conditions.

Links

EF16_013/0001761, research and development project

Name: RECETOX RI

GA16-21649S, research and development project

Name: Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

Investor: Czech Science Foundation

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/1087/2018, interní kód MU

Name: Molekulární a buněčná biologie pro biomedicínské vědy

Investor: Masaryk University, Category A

Citovat

BOSÁK, Juraj, Matěj HRALA, Viktória PIRKOVÁ, Lenka MICENKOVÁ, Alois CIZEK, Jiri SMOLA, Dana KUCEROVA, Zdenka VACKOVA, Eva BUDINSKÁ, Ivana KOLÁČKOVÁ and David ŠMAJS. Porcine pathogenic Escherichia coli strains differ from human fecal strains in occurrence of bacteriocin types. Veterinary Microbiology. AMSTERDAM: Elsevier, 2019, vol. 232, MAY 2019, p. 121-127. ISSN 0378-1135. Available from: https://dx.doi.org/10.1016/j.vetmic.2019.04.003.

@article{1603839,
   author = {Bosák, Juraj and Hrala, Matěj and Pirková, Viktória and Micenková, Lenka and Cizek, Alois and Smola, Jiri and Kucerova, Dana and Vackova, Zdenka and Budinská, Eva and Koláčková, Ivana and Šmajs, David},
   article_location = {AMSTERDAM},
   article_number = {MAY 2019},
   doi = {http://dx.doi.org/10.1016/j.vetmic.2019.04.003},
   keywords = {Bacteriocin; Colicin; E. coli; STEC; ETEC; Pig},
   language = {eng},
   issn = {0378-1135},
   journal = {Veterinary Microbiology},
   title = {Porcine pathogenic Escherichia coli strains differ from human fecal strains in occurrence of bacteriocin types},
   url = {http://dx.doi.org/10.1016/j.vetmic.2019.04.003},
   volume = {232},
   year = {2019}
}

TY  - JOUR
ID  - 1603839
AU  - Bosák, Juraj - Hrala, Matěj - Pirková, Viktória - Micenková, Lenka - Cizek, Alois - Smola, Jiri - Kucerova, Dana - Vackova, Zdenka - Budinská, Eva - Koláčková, Ivana - Šmajs, David
PY  - 2019
TI  - Porcine pathogenic Escherichia coli strains differ from human fecal strains in occurrence of bacteriocin types
JF  - Veterinary Microbiology
VL  - 232
IS  - MAY 2019
SP  - 121-127
EP  - 121-127
PB  - Elsevier
SN  - 03781135
KW  - Bacteriocin
KW  - Colicin
KW  - E. coli
KW  - STEC
KW  - ETEC
KW  - Pig
UR  - http://dx.doi.org/10.1016/j.vetmic.2019.04.003
L2  - http://dx.doi.org/10.1016/j.vetmic.2019.04.003
N2  - Enterotoxigenic and Shiga-toxigenic Escherichia coli (i.e., ETEC and STEC) are important causative agents of human and animal diseases. In humans, infections range from mild diarrhea to severe life-threating conditions, while infections of piglets result in lower weight gain and higher pig mortality with the accompanying significant economic losses. In this study, frequencies of four phylogenetic groups, fourteen virulence- and thirty bacteriocin determinants were analyzed in a set of 443 fecal E. coli isolates from diseased pigs and compared to a previously characterized set of 1283 human fecal E. coli isolates collected in the same geographical region. In addition, these characteristics were compared among ETEC, STEC, and non-toxigenic porcine E. coli isolates. Phylogenetic group A was prevalent among porcine pathogenic E. coli isolates, whereas the frequency of phylogroup B2, adhesion/invasion (firnA, pap, sfa, afaI, ial, ipaH, and pCVD432) and iron acquisition (aer and iucC) determinants were less frequent compared to human fecal isolates. Additionally, porcine isolates differed from human isolates relative to the spectrum of produced bacteriocins. While human fecal isolates encoded colicins and microcins with a similar prevalence, porcine pathogenic E. coli isolates produced predominantly colicins (94% of isolates); especially colicins B (42.6%), M (40.1%), and Ib (34.0%), which are encoded on large conjugative plasmids. The observed high prevalence of these colicin determinants suggests the importance of large colicinogenic plasmids and/or the importance of colicin production in intestinal inflammatory conditions.
ER  -

BOSÁK, Juraj, Matěj HRALA, Viktória PIRKOVÁ, Lenka MICENKOVÁ, Alois CIZEK, Jiri SMOLA, Dana KUCEROVA, Zdenka VACKOVA, Eva BUDINSKÁ, Ivana KOLÁČKOVÁ and David ŠMAJS. Porcine pathogenic Escherichia coli strains differ from human fecal strains in occurrence of bacteriocin types. \textit{Veterinary Microbiology}. AMSTERDAM: Elsevier, 2019, vol.~232, MAY 2019, p.~121-127. ISSN~0378-1135. Available from: https://dx.doi.org/10.1016/j.vetmic.2019.04.003.

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