A public database for the new MLST scheme for Treponema
pallidum subsp. pallidum: surveillance and epidemiology of the
causative agent of syphilis

J 2019

A public database for the new MLST scheme for Treponema pallidum subsp. pallidum: surveillance and epidemiology of the causative agent of syphilis

GRILLOVÁ, Linda, Keith JOLLEY, David ŠMAJS and Mathieu PICARDEAU

Basic information

Original name

A public database for the new MLST scheme for Treponema pallidum subsp. pallidum: surveillance and epidemiology of the causative agent of syphilis

Authors

GRILLOVÁ, Linda (203 Czech Republic, guarantor), Keith JOLLEY (826 United Kingdom of Great Britain and Northern Ireland), David ŠMAJS (203 Czech Republic, belonging to the institution) and Mathieu PICARDEAU (250 France)

Edition

PeerJ, London, PEERJ INC, 2019, 2167-8359

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.379

RIV identification code

RIV/00216224:14110/19:00108563

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.7717/peerj.6182

UT WoS

000455261800007

Keywords in English

Treponema pallidum subsp. pallidum; Molecular typing; PubMLST

Abstract

V originále

Treponema pallidum subsp. pallidum is the causative agent of syphilis, a sexually transmitted disease with worldwide prevalence. Several different molecular typing schemes are currently available for this pathogen. To enable population biology studies of the syphilis agent and for epidemiological surveillance at the global scale, a harmonized typing tool needs to be introduced. Recently, we published a new multi-locus sequence typing (MLST) with the potential to significantly enhance the epidemiological data in several aspects (e.g., distinguishing genetically different clades of syphilis, subtyping inside these clades, and finally, distinguishing different subspecies of non-cultivable pathogenic treponemes). In this short report, we introduce the PubMLST database for treponemal DNA data storage and for assignments of allelic profiles and sequencing types. Moreover, we have summarized epidemiological data of all treponemal strains (n = 358) with available DNA sequences in typing loci and found several association between genetic groups and characteristics of patients. This study proposes the establishment of a single MLST of T. p. pallidum and encourages researchers and public health communities to use this PubMLST database as a universal tool for molecular typing studies of the syphilis pathogen.

Links

MUNI/A/1087/2018, interní kód MU

Name: Molekulární a buněčná biologie pro biomedicínské vědy

Investor: Masaryk University, Category A

NV17-31333A, research and development project

Name: Vývoj nového typovacího systému pro původce syfilis, Treponema pallidum subsp. pallidum, zaměřeného na proteomické rozdíly

Citovat

GRILLOVÁ, Linda, Keith JOLLEY, David ŠMAJS and Mathieu PICARDEAU. A public database for the new MLST scheme for Treponema pallidum subsp. pallidum: surveillance and epidemiology of the causative agent of syphilis. PeerJ. London: PEERJ INC, 2019, vol. 6, JAN 9 2019, p. 1-13. ISSN 2167-8359. Available from: https://dx.doi.org/10.7717/peerj.6182.

@article{1603885,
   author = {Grillová, Linda and Jolley, Keith and Šmajs, David and Picardeau, Mathieu},
   article_location = {London},
   article_number = {JAN 9 2019},
   doi = {http://dx.doi.org/10.7717/peerj.6182},
   keywords = {Treponema pallidum subsp. pallidum; Molecular typing; PubMLST},
   language = {eng},
   issn = {2167-8359},
   journal = {PeerJ},
   title = {A public database for the new MLST scheme for Treponema pallidum subsp. pallidum: surveillance and epidemiology of the causative agent of syphilis},
   url = {http://dx.doi.org/10.7717/peerj.6182},
   volume = {6},
   year = {2019}
}

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AU  - Grillová, Linda - Jolley, Keith - Šmajs, David - Picardeau, Mathieu
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JF  - PeerJ
VL  - 6
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EP  - 1-13
PB  - PEERJ INC
SN  - 21678359
KW  - Treponema pallidum subsp. pallidum
KW  - Molecular typing
KW  - PubMLST
UR  - http://dx.doi.org/10.7717/peerj.6182
L2  - http://dx.doi.org/10.7717/peerj.6182
N2  - Treponema pallidum subsp. pallidum is the causative agent of syphilis, a sexually transmitted disease with worldwide prevalence. Several different molecular typing schemes are currently available for this pathogen. To enable population biology studies of the syphilis agent and for epidemiological surveillance at the global scale, a harmonized typing tool needs to be introduced. Recently, we published a new multi-locus sequence typing (MLST) with the potential to significantly enhance the epidemiological data in several aspects (e.g., distinguishing genetically different clades of syphilis, subtyping inside these clades, and finally, distinguishing different subspecies of non-cultivable pathogenic treponemes). In this short report, we introduce the PubMLST database for treponemal DNA data storage and for assignments of allelic profiles and sequencing types. Moreover, we have summarized epidemiological data of all treponemal strains (n = 358) with available DNA sequences in typing loci and found several association between genetic groups and characteristics of patients. This study proposes the establishment of a single MLST of T. p. pallidum and encourages researchers and public health communities to use this PubMLST database as a universal tool for molecular typing studies of the syphilis pathogen.
ER  -

GRILLOVÁ, Linda, Keith JOLLEY, David ŠMAJS and Mathieu PICARDEAU. A public database for the new MLST scheme for Treponema pallidum subsp. pallidum: surveillance and epidemiology of the causative agent of syphilis. \textit{PeerJ}. London: PEERJ INC, 2019, vol.~6, JAN 9 2019, p.~1-13. ISSN~2167-8359. Available from: https://dx.doi.org/10.7717/peerj.6182.

Detailed Information on Publication Record