J 2019

Sumoylation regulates the stability and nuclease activity of Saccharomyces cerevisiae Dna2

RANJHA, Lepakshi, Maryna LEVIKOVA, Veronika ALTMANNOVÁ, Lumír KREJČÍ, Petr CEJKA et. al.

Basic information

Original name

Sumoylation regulates the stability and nuclease activity of Saccharomyces cerevisiae Dna2

Authors

RANJHA, Lepakshi (756 Switzerland), Maryna LEVIKOVA (756 Switzerland), Veronika ALTMANNOVÁ (203 Czech Republic, belonging to the institution), Lumír KREJČÍ (203 Czech Republic, belonging to the institution) and Petr CEJKA (guarantor)

Edition

COMMUNICATIONS BIOLOGY, NEW YORK, NATURE PUBLISHING GROUP, 2019, 2399-3642

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 4.165

RIV identification code

RIV/00216224:14110/19:00108020

Organization unit

Faculty of Medicine

UT WoS

000467215700004

Keywords in English

SINGLE-STRANDED-DNA; END-RESECTION; IN-VIVO; SECONDARY STRUCTURE; OKAZAKI FRAGMENTS; REPLICATION FORKS; EXONUCLEASE 1; SUMO; HELICASE; PROTEIN

Tags

Tags

International impact, Reviewed
Změněno: 4/3/2020 14:24, Mgr. Tereza Miškechová

Abstract

V originále

Dna2 is an essential nuclease-helicase that acts in several distinct DNA metabolic pathways including DNA replication and recombination. To balance these functions and prevent unscheduled DNA degradation, Dna2 activities must be regulated. Here we show that Saccharomyces cerevisiae Dna2 function is controlled by sumoylation. We map the sumoylation sites to the N-terminal regulatory domain of Dna2 and show that in vitro sumoylation of recombinant Dna2 impairs its nuclease but not helicase activity. In cells, the total levels of the non-sumoylatable Dna2 variant are elevated. However, non-sumoylatable Dna2 shows impaired nuclear localization and reduced recruitment to foci upon DNA damage. Non-sumoylatable Dna2 reduces the rate of DNA end resection, as well as impedes cell growth and cell cycle progression through S phase. Taken together, these findings show that in addition to Dna2 phosphorylation described previously, Dna2 sumoylation is required for the homeostasis of the Dna2 protein function to promote genome stability.

Links

EF16_025/0007381, research and development project
Name: Preklinická progrese nových organických sloučenin s cílenou biologickou aktivitou
GA17-17720S, research and development project
Name: Vnitřní vlastnosti RAD51 vlákna a jeho biologické regulace
Investor: Czech Science Foundation
MUNI/A/1087/2018, interní kód MU
Name: Molekulární a buněčná biologie pro biomedicínské vědy
Investor: Masaryk University, Category A
206292/E/17/Z, interní kód MU
Name: Mechanics and execution of homologous recombination - biophysics to the organism
Investor: Wellcome Trust