Detailed Information on Publication Record
2019
Sumoylation regulates the stability and nuclease activity of Saccharomyces cerevisiae Dna2
RANJHA, Lepakshi, Maryna LEVIKOVA, Veronika ALTMANNOVÁ, Lumír KREJČÍ, Petr CEJKA et. al.Basic information
Original name
Sumoylation regulates the stability and nuclease activity of Saccharomyces cerevisiae Dna2
Authors
RANJHA, Lepakshi (756 Switzerland), Maryna LEVIKOVA (756 Switzerland), Veronika ALTMANNOVÁ (203 Czech Republic, belonging to the institution), Lumír KREJČÍ (203 Czech Republic, belonging to the institution) and Petr CEJKA (guarantor)
Edition
COMMUNICATIONS BIOLOGY, NEW YORK, NATURE PUBLISHING GROUP, 2019, 2399-3642
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10608 Biochemistry and molecular biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 4.165
RIV identification code
RIV/00216224:14110/19:00108020
Organization unit
Faculty of Medicine
UT WoS
000467215700004
Keywords in English
SINGLE-STRANDED-DNA; END-RESECTION; IN-VIVO; SECONDARY STRUCTURE; OKAZAKI FRAGMENTS; REPLICATION FORKS; EXONUCLEASE 1; SUMO; HELICASE; PROTEIN
Tags
Tags
International impact, Reviewed
Změněno: 4/3/2020 14:24, Mgr. Tereza Miškechová
Abstract
V originále
Dna2 is an essential nuclease-helicase that acts in several distinct DNA metabolic pathways including DNA replication and recombination. To balance these functions and prevent unscheduled DNA degradation, Dna2 activities must be regulated. Here we show that Saccharomyces cerevisiae Dna2 function is controlled by sumoylation. We map the sumoylation sites to the N-terminal regulatory domain of Dna2 and show that in vitro sumoylation of recombinant Dna2 impairs its nuclease but not helicase activity. In cells, the total levels of the non-sumoylatable Dna2 variant are elevated. However, non-sumoylatable Dna2 shows impaired nuclear localization and reduced recruitment to foci upon DNA damage. Non-sumoylatable Dna2 reduces the rate of DNA end resection, as well as impedes cell growth and cell cycle progression through S phase. Taken together, these findings show that in addition to Dna2 phosphorylation described previously, Dna2 sumoylation is required for the homeostasis of the Dna2 protein function to promote genome stability.
Links
EF16_025/0007381, research and development project |
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GA17-17720S, research and development project |
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MUNI/A/1087/2018, interní kód MU |
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206292/E/17/Z, interní kód MU |
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