J 2019

Freshwater Cyanotoxin Cylindrospermopsin Has Detrimental Stage-specific Effects on Hepatic Differentiation From Human Embryonic Stem Cells

VÁŇOVÁ, Tereza, Jan RAŠKA, Pavel BABICA, Iva SOVADINOVÁ, Michaela BOSÁKOVÁ et. al.

Basic information

Original name

Freshwater Cyanotoxin Cylindrospermopsin Has Detrimental Stage-specific Effects on Hepatic Differentiation From Human Embryonic Stem Cells

Authors

VÁŇOVÁ, Tereza (203 Czech Republic, belonging to the institution), Jan RAŠKA (203 Czech Republic, belonging to the institution), Pavel BABICA (203 Czech Republic, belonging to the institution), Iva SOVADINOVÁ (203 Czech Republic, belonging to the institution), Michaela BOSÁKOVÁ (203 Czech Republic, belonging to the institution), Petr DVOŘÁK (203 Czech Republic, belonging to the institution), Luděk BLÁHA (203 Czech Republic, belonging to the institution) and Vladimír ROTREKL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Toxicological sciences, OXFORD, Academic Press, 2019, 1096-6080

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30108 Toxicology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 3.703

RIV identification code

RIV/00216224:14110/19:00108022

Organization unit

Faculty of Medicine

UT WoS

000462865100021

Keywords in English

cylindrospermopsin; hepatic differentiation; human embryonic stem cells; liver; cyanotoxin

Tags

International impact, Reviewed
Změněno: 4/3/2020 14:06, Mgr. Tereza Miškechová

Abstract

V originále

Cylindrospermopsin (CYN) has been recognized as a potent waterborne hepatotoxin with an increasing environmental occurrence. However, CYN effects on the specific populations of hepatic cells involved in liver tissue development, renewal, and regeneration, have not been characterized yet. We used human embryonic stem cells to analyze the hepatic differentiation stage-specific effect of CYN. Our results strongly suggest that CYN might contribute to the development of chronic adverse outcomes by disrupting liver tissue homeostasis in terms of (1) cellular stress and damage induced in the mature differentiated hepatocytes, which was associated with a necrotic cell death and thus possibly also inflammatory responses; (2) selective elimination of HNF4+ cells from populations of progenitor cells and immature hepatocytes during hepatic differentiation, which could possibly lead to an impaired liver renewal and regeneration; (3) impaired hepatic functions of immature hepatocytes, such as decreased albumin secretion or increased lipid accumulation, which could contribute to the development of liver steatosis; and (4) survival of the immature and AFP-expressing cells with the limited ability to further differentiate, which could represent a tumor-promoting condition.

Links

EF16_013/0001761, research and development project
Name: RECETOX RI
GA15-12408S, research and development project
Name: Role kmenových a diferencovaných jaterních buněk v hepatotoxicitě a hepatokarcinogenitě indukované cyanotoxiny
Investor: Czech Science Foundation
GA15-23033S, research and development project
Name: Úloha sekrece FGF2 ve fyziologii lidských pluripotentních kmenových buněk
Investor: Czech Science Foundation
GBP302/12/G157, research and development project
Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii
Investor: Czech Science Foundation
LM2015051, research and development project
Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)
Investor: Ministry of Education, Youth and Sports of the CR
LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
MUNI/A/1087/2018, interní kód MU
Name: Molekulární a buněčná biologie pro biomedicínské vědy
Investor: Masaryk University, Category A