Detailed Information on Publication Record
2019
Obesity-induced nucleosome release predicts poor cardio-metabolic health
LO RE, Oriana, Andrea MAUGERI, Jana HRUŠKOVÁ, Juraj JAKUBÍK, Jan KUČERA et. al.Basic information
Original name
Obesity-induced nucleosome release predicts poor cardio-metabolic health
Authors
LO RE, Oriana (380 Italy, belonging to the institution), Andrea MAUGERI (380 Italy), Jana HRUŠKOVÁ (203 Czech Republic), Juraj JAKUBÍK (703 Slovakia), Jan KUČERA (203 Czech Republic, belonging to the institution), Julie DOBROVOLNÁ (203 Czech Republic, belonging to the institution), Jude A. OBEN (826 United Kingdom of Great Britain and Northern Ireland), Lukáš KUBALA (203 Czech Republic, belonging to the institution), Adéla DVOŘÁKOVÁ (203 Czech Republic, belonging to the institution), Milan ČÍŽ (203 Czech Republic, belonging to the institution) and Manlio VINCIGUERRA (380 Italy, guarantor)
Edition
CLINICAL EPIGENETICS, LONDON, BMC, 2019, 1868-7075
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30101 Human genetics
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 5.028
RIV identification code
RIV/00216224:14110/19:00112410
Organization unit
Faculty of Medicine
UT WoS
000505032600001
Keywords in English
Liquid biopsy; Nucleosome; Epigenetics; Metabolic health; Cardiovascular disease
Tags
International impact, Reviewed
Změněno: 17/2/2023 11:31, Mgr. Marie Šípková, DiS.
Abstract
V originále
Objective While circulating nucleosome levels are high in obese mouse models, it is unknown where these nucleosomes originate from and whether they are a marker of cardio-metabolic health in humans. Here, we aimed to determine whether an association exists between circulating nucleosomes and the risk of developing obesity, metabolic syndrome (MetS) and/or a dysfunctional cardiovascular performance. Methods We randomly selected 120 participants of the Kardiovize Brno 2030 study across three BMI strata: BMI 18-25, 25-30, and > 30. We assessed the association between circulating nucleosome levels and the risk of obesity, MetS, and poor cardiovascular health. We then cultured human neutrophils, adipocytes, and hepatoma cells to study nucleosome origins in a fat-rich environment. Results Circulating nucleosome levels positively correlated with BMI (R = 0.602, p < 0.05), fatty liver index (R = 0.622, p < 0.05), left ventricular mass (R = 0.457, p < 0.05), and associated with MetS (p < 0.001) and poor cardiovascular health (p < 0.001). Incubating neutrophils with 1-10 mu M free fatty acids triggered nucleosome production without concomitant cell death. Nucleosomes were not produced during pre-adipocyte differentiation or upon incubation of hepatic cells with palmitic acid. Conclusions Neutrophils are a bona fide source of circulating nucleosomes in an obesogenic environment and in overweight/obese patients. High nucleosome levels are associated with MetS and cardiovascular performance, and might represent novel candidate biomarkers for cardio-metabolic health.
Links
| MUNI/A/1553/2018, interní kód MU |
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