Identification of a Diagnostic Set of Endomyocardial Biopsy
microRNAs for Acute Cellular Rejection Diagnostics in Patients
after Heart Transplantation Using Next-Generation Sequencing

J 2019

Identification of a Diagnostic Set of Endomyocardial Biopsy microRNAs for Acute Cellular Rejection Diagnostics in Patients after Heart Transplantation Using Next-Generation Sequencing

NOVÁKOVÁ, Tereza, Táňa MACHÁČKOVÁ, Jan NOVÁK, Petr HUDE, Július GODAVA et. al.

Basic information

Original name

Identification of a Diagnostic Set of Endomyocardial Biopsy microRNAs for Acute Cellular Rejection Diagnostics in Patients after Heart Transplantation Using Next-Generation Sequencing

Authors

NOVÁKOVÁ, Tereza (203 Czech Republic, belonging to the institution), Táňa MACHÁČKOVÁ (203 Czech Republic, belonging to the institution), Jan NOVÁK (203 Czech Republic, belonging to the institution), Petr HUDE (203 Czech Republic, belonging to the institution), Július GODAVA (703 Slovakia, belonging to the institution), Víta ŽAMPACHOVÁ (203 Czech Republic, belonging to the institution), Jan OPPELT (203 Czech Republic, belonging to the institution), Filip ZLÁMAL (203 Czech Republic, belonging to the institution), Petr NEMEC (203 Czech Republic), Helena BEDANOVA (203 Czech Republic), Ondřej SLABÝ (203 Czech Republic, belonging to the institution), Julie DOBROVOLNÁ (203 Czech Republic, belonging to the institution), Lenka ŠPINAROVÁ (203 Czech Republic, belonging to the institution) and Jan KREJČÍ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Cells, Basel, MDPI, 2019, 2073-4409

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.366

RIV identification code

RIV/00216224:14110/19:00108567

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/cells8111400

UT WoS

000502266700098

Keywords in English

microRNA; endomyocardial biopsy; heart transplantation; acute cellular rejection; diagnostics

Abstract

V originále

Introduction: Acute cellular rejection (ACR) of heart allografts represents the most common reason for graft failure. Endomyocardial biopsies (EMB) are still subject to substantial interobserver variability. Novel biomarkers enabling precise ACR diagnostics may decrease interobserver variability. We aimed to identify a specific subset of microRNAs reflecting the presence of ACR. Patients and Methods: Monocentric retrospective study. A total of 38 patients with the anamnesis of ACR were identified and for each patient three consecutive samples of EMB (with, prior and after ACR) were collected. Sixteen trios were used for next-generation sequencing (exploratory cohort); the resting 22 trios were used for validation with qRT-PCR (validation cohort). Statistical analysis was performed using R software. Results: The analysis of the exploration cohort provided the total of 11 miRNAs that were altered during ACR, the three of which (miR-144, miR-589 and miR-182) were further validated in the validation cohort. Using the levels of all 11 miRNAs and principal component analysis, an ACR score was created with the specificity of 91% and sensitivity of 68% for detecting the presence of ACR in the EMB sample. Conclusion: We identified a set of microRNAs altered in endomyocardial biopsies during ACR and using their relative levels we created a diagnostic score that can be used for ACR diagnosis.

Links

MUNI/A/1553/2018, interní kód MU

Name: Genetické, environmentální a tkáňové charakteristiky vybraných patologických stavů a nemocí (Acronym: genetika; stres; biomateriály; tkáňové kultury)

Investor: Masaryk University, Category A

NV16-30537A, research and development project

Name: Cirkulující mikroRNA jako neinvazivní markery rejekce štěpu u pacientů po srdeční transplantaci

90091, large research infrastructures

Name: NCMG

Citovat

NOVÁKOVÁ, Tereza, Táňa MACHÁČKOVÁ, Jan NOVÁK, Petr HUDE, Július GODAVA, Víta ŽAMPACHOVÁ, Jan OPPELT, Filip ZLÁMAL, Petr NEMEC, Helena BEDANOVA, Ondřej SLABÝ, Julie DOBROVOLNÁ, Lenka ŠPINAROVÁ and Jan KREJČÍ. Identification of a Diagnostic Set of Endomyocardial Biopsy microRNAs for Acute Cellular Rejection Diagnostics in Patients after Heart Transplantation Using Next-Generation Sequencing. Cells. Basel: MDPI, 2019, vol. 8, No 11, p. 1-13. ISSN 2073-4409. Available from: https://dx.doi.org/10.3390/cells8111400.

@article{1604797,
   author = {Nováková, Tereza and Macháčková, Táňa and Novák, Jan and Hude, Petr and Godava, Július and Žampachová, Víta and Oppelt, Jan and Zlámal, Filip and Nemec, Petr and Bedanova, Helena and Slabý, Ondřej and Dobrovolná, Julie and Špinarová, Lenka and Krejčí, Jan},
   article_location = {Basel},
   article_number = {11},
   doi = {http://dx.doi.org/10.3390/cells8111400},
   keywords = {microRNA; endomyocardial biopsy; heart transplantation; acute cellular rejection; diagnostics},
   language = {eng},
   issn = {2073-4409},
   journal = {Cells},
   title = {Identification of a Diagnostic Set of Endomyocardial Biopsy microRNAs for Acute Cellular Rejection Diagnostics in Patients after Heart Transplantation Using Next-Generation Sequencing},
   url = {http://dx.doi.org/10.3390/cells8111400},
   volume = {8},
   year = {2019}
}

TY  - JOUR
ID  - 1604797
AU  - Nováková, Tereza - Macháčková, Táňa - Novák, Jan - Hude, Petr - Godava, Július - Žampachová, Víta - Oppelt, Jan - Zlámal, Filip - Nemec, Petr - Bedanova, Helena - Slabý, Ondřej - Dobrovolná, Julie - Špinarová, Lenka - Krejčí, Jan
PY  - 2019
TI  - Identification of a Diagnostic Set of Endomyocardial Biopsy microRNAs for Acute Cellular Rejection Diagnostics in Patients after Heart Transplantation Using Next-Generation Sequencing
JF  - Cells
VL  - 8
IS  - 11
SP  - 1-13
EP  - 1-13
PB  - MDPI
SN  - 20734409
KW  - microRNA
KW  - endomyocardial biopsy
KW  - heart transplantation
KW  - acute cellular rejection
KW  - diagnostics
UR  - http://dx.doi.org/10.3390/cells8111400
L2  - http://dx.doi.org/10.3390/cells8111400
N2  - Introduction: Acute cellular rejection (ACR) of heart allografts represents the most common reason for graft failure. Endomyocardial biopsies (EMB) are still subject to substantial interobserver variability. Novel biomarkers enabling precise ACR diagnostics may decrease interobserver variability. We aimed to identify a specific subset of microRNAs reflecting the presence of ACR. Patients and Methods: Monocentric retrospective study. A total of 38 patients with the anamnesis of ACR were identified and for each patient three consecutive samples of EMB (with, prior and after ACR) were collected. Sixteen trios were used for next-generation sequencing (exploratory cohort); the resting 22 trios were used for validation with qRT-PCR (validation cohort). Statistical analysis was performed using R software. Results: The analysis of the exploration cohort provided the total of 11 miRNAs that were altered during ACR, the three of which (miR-144, miR-589 and miR-182) were further validated in the validation cohort. Using the levels of all 11 miRNAs and principal component analysis, an ACR score was created with the specificity of 91% and sensitivity of 68% for detecting the presence of ACR in the EMB sample. Conclusion: We identified a set of microRNAs altered in endomyocardial biopsies during ACR and using their relative levels we created a diagnostic score that can be used for ACR diagnosis.
ER  -

NOVÁKOVÁ, Tereza, Táňa MACHÁČKOVÁ, Jan NOVÁK, Petr HUDE, Július GODAVA, Víta ŽAMPACHOVÁ, Jan OPPELT, Filip ZLÁMAL, Petr NEMEC, Helena BEDANOVA, Ondřej SLABÝ, Julie DOBROVOLNÁ, Lenka ŠPINAROVÁ and Jan KREJČÍ. Identification of a Diagnostic Set of Endomyocardial Biopsy microRNAs for Acute Cellular Rejection Diagnostics in Patients after Heart Transplantation Using Next-Generation Sequencing. \textit{Cells}. Basel: MDPI, 2019, vol.~8, No~11, p.~1-13. ISSN~2073-4409. Available from: https://dx.doi.org/10.3390/cells8111400.

Detailed Information on Publication Record