| SKUBALOVA, Zuzana, Hana MICHALKOVA, Petr MICHALEK, Vladislav STRMISKA, Roman GURAN, Miguel Angel Merlos RODRIGO, Klara CASTKOVA, David HYNEK, Vladimír PEKAŘÍK, Ondrej ZITKA, Vojtech ADAM and Zbynek HEGER. Prevalent anatase crystalline phase increases the cytotoxicity of biphasic titanium dioxide nanoparticles in mammalian cells. Colloids and Surfaces B: Biointerfaces. Amsterdam: Elsevier, 2019, vol. 182, OCT 1 2019, p. 1-10. ISSN 0927-7765. Available from: https://dx.doi.org/10.1016/j.colsurfb.2019.110391. |
Other formats:
BibTeX
LaTeX
RIS
@article{1604897,
author = {Skubalova, Zuzana and Michalkova, Hana and Michalek, Petr and Strmiska, Vladislav and Guran, Roman and Rodrigo, Miguel Angel Merlos and Castkova, Klara and Hynek, David and Pekařík, Vladimír and Zitka, Ondrej and Adam, Vojtech and Heger, Zbynek},
article_location = {Amsterdam},
article_number = {OCT 1 2019},
doi = {http://dx.doi.org/10.1016/j.colsurfb.2019.110391},
keywords = {Cytotoxicity; Nanotoxicology; Oxidative stress; Proteomics; Titanium},
language = {eng},
issn = {0927-7765},
journal = {Colloids and Surfaces B: Biointerfaces},
title = {Prevalent anatase crystalline phase increases the cytotoxicity of biphasic titanium dioxide nanoparticles in mammalian cells},
url = {http://dx.doi.org/10.1016/j.colsurfb.2019.110391},
volume = {182},
year = {2019}
}
TY - JOUR
ID - 1604897
AU - Skubalova, Zuzana - Michalkova, Hana - Michalek, Petr - Strmiska, Vladislav - Guran, Roman - Rodrigo, Miguel Angel Merlos - Castkova, Klara - Hynek, David - Pekařík, Vladimír - Zitka, Ondrej - Adam, Vojtech - Heger, Zbynek
PY - 2019
TI - Prevalent anatase crystalline phase increases the cytotoxicity of biphasic titanium dioxide nanoparticles in mammalian cells
JF - Colloids and Surfaces B: Biointerfaces
VL - 182
IS - OCT 1 2019
SP - 1-10
EP - 1-10
PB - Elsevier
SN - 09277765
KW - Cytotoxicity
KW - Nanotoxicology
KW - Oxidative stress
KW - Proteomics
KW - Titanium
UR - http://dx.doi.org/10.1016/j.colsurfb.2019.110391
L2 - http://dx.doi.org/10.1016/j.colsurfb.2019.110391
N2 - Nanoparticular form of titanium dioxide (TiO2 NPs) belongs to important industrial material. Despite being widely used, serious contradictions regarding biosafety of TiO2 NPs remain. We anticipate that such discrepancies could be due to a lack of understanding of a linkage between TiO2 NPs phase composition and cytotoxicity. Therefore, we synthesized two types of biphasic TiO2 NPs differing in an anatase-brookite phase composition. The study presents an array of in vitro data suggesting that TiO2 NPs with a prevailing anatase phase composition possess higher cytotoxicity compared to TiO2 NPs with an equal anatase-brookite crystallinity. This phenomenon was evidenced by significantly higher inhibition of metabolic activity and growth of epithelial and neuroblast-like cells. Moreover, anatase-prevailing TiO2 NPs tend to produce higher amount of reactive oxygen species resulting in DNA fragmentation. Further insights into the molecular aspects of cytotoxicity of anatase-prevailing TiO2 NPs were obtained by comparative proteomics delineating that TiO2 NPs deregulate expression of a variety of proteins and associated pathways. This inevitably results in a decreased cellular ability to detoxify reactive oxygen species and respond to various stress conditions. The study provides novel data that add another piece to the jigsaw of the relation between structural features of NPs and biosafety.
ER -
SKUBALOVA, Zuzana, Hana MICHALKOVA, Petr MICHALEK, Vladislav STRMISKA, Roman GURAN, Miguel Angel Merlos RODRIGO, Klara CASTKOVA, David HYNEK, Vladimír PEKAŘÍK, Ondrej ZITKA, Vojtech ADAM and Zbynek HEGER. Prevalent anatase crystalline phase increases the cytotoxicity of biphasic titanium dioxide nanoparticles in mammalian cells. \textit{Colloids and Surfaces B: Biointerfaces}. Amsterdam: Elsevier, 2019, vol.~182, OCT 1 2019, p.~1-10. ISSN~0927-7765. Available from: https://dx.doi.org/10.1016/j.colsurfb.2019.110391.
|
