J 2019

Generation and characterization of DSPP-Cerulean/DMP1-Cherry reporter mice

VIJAYKUMAR, Anushree, Sean GHASSEM-ZADEH, Ivana VIDOVIC-ZDRILIC, Karren KOMITAS, Igor ADAMEYKO et. al.

Basic information

Original name

Generation and characterization of DSPP-Cerulean/DMP1-Cherry reporter mice

Authors

VIJAYKUMAR, Anushree (840 United States of America), Sean GHASSEM-ZADEH (840 United States of America), Ivana VIDOVIC-ZDRILIC (840 United States of America), Karren KOMITAS (840 United States of America), Igor ADAMEYKO (752 Sweden), Jan KŘIVÁNEK (203 Czech Republic, belonging to the institution), Yu FU (840 United States of America), Peter MAYE (840 United States of America) and Mina MINA (840 United States of America, guarantor)

Edition

GENESIS, MALDEN, WILEY-BLACKWELL, 2019, 1526-954X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10603 Genetics and heredity

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 1.760

RIV identification code

RIV/00216224:14110/19:00112451

Organization unit

Faculty of Medicine

DOI

UT WoS

000489695700005

Keywords in English

bone; dentin matrix protein 1; dentin sialophosphoprotein; fluorescent protein reporters; Odontoblasts

Tags

Tags

International impact, Reviewed
Změněno: 16/1/2020 10:57, Mgr. Tereza Miškechová

Abstract

V originále

To gain a better understanding of the progression of progenitor cells in the odontoblast lineage, we have examined and characterized the expression of a series of GFP reporters during odontoblast differentiation. However, previously reported GFP reporters (pOBCol2.3-GFP, pOBCol3.6-GFP, and DMP1-GFP), similar to the endogenous proteins, are also expressed by bone-forming cells, which made it difficult to delineate the two cell types in various in vivo and in vitro studies. To overcome these difficulties we generated DSPP-Cerulean/DMP1-Cherry transgenic mice using a bacterial recombination strategy with the mouse BAC clone RP24-258g7. We have analyzed the temporal and spatial expression of both transgenes in tooth and bone in vivo and in vitro. This transgenic animal enabled us to visualize the interactions between odontoblasts and surrounding tissues including dental pulp, ameloblasts and cementoblasts. Our studies showed that DMP1-Cherry, similar to Dmp1, was expressed in functional and fully differentiated odontoblasts as well as osteoblasts, osteocytes and cementoblasts. Expression of DSPP-Cerulean transgene was limited to functional and fully differentiated odontoblasts and correlated with the expression of Dspp. This transgenic animal can help in the identification and isolation of odontoblasts at later stages of differentiation and help in better understanding of developmental disorders in dentin and odontoblasts.