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@article{1604997,
author = {Vychytilová, Petra and Svobodová Kovaříková, Alena and Grolich, Tomáš and Procházka, Vladimír and Slabá, Kateřina and Macháčková, Táňa and Halámková, Jana and Svoboda, Marek and Kala, Zdeněk and Kiss, Igor and Slabý, Ondřej},
article_location = {Basel},
article_number = {18},
doi = {http://dx.doi.org/10.3390/ijms20184460},
keywords = {microRNA; biogenesis; colorectal cancer; disease-free survival; overall survival; RT-qPCR},
language = {eng},
issn = {1422-0067},
journal = {International Journal of Molecular Sciences},
title = {MicroRNA Biogenesis Pathway Genes Are Deregulated in Colorectal Cancer},
url = {https://www.mdpi.com/1422-0067/20/18/4460},
volume = {20},
year = {2019}
}
TY - JOUR
ID - 1604997
AU - Vychytilová, Petra - Svobodová Kovaříková, Alena - Grolich, Tomáš - Procházka, Vladimír - Slabá, Kateřina - Macháčková, Táňa - Halámková, Jana - Svoboda, Marek - Kala, Zdeněk - Kiss, Igor - Slabý, Ondřej
PY - 2019
TI - MicroRNA Biogenesis Pathway Genes Are Deregulated in Colorectal Cancer
JF - International Journal of Molecular Sciences
VL - 20
IS - 18
SP - 1-13
EP - 1-13
PB - Multidisciplinary Digital Publishing Institute
SN - 14220067
KW - microRNA
KW - biogenesis
KW - colorectal cancer
KW - disease-free survival
KW - overall survival
KW - RT-qPCR
UR - https://www.mdpi.com/1422-0067/20/18/4460
L2 - https://www.mdpi.com/1422-0067/20/18/4460
N2 - MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression. Each step of their production and maturation has to be strictly regulated, as any disruption of control mechanisms may lead to cancer. Thus, we have measured the expression of 19 genes involved in miRNAs biogenesis pathway in tumor tissues of 239 colorectal cancer (CRC) patients, 17 CRC patients with liver metastases and 239 adjacent tissues using real-time PCR. Subsequently, the expression of analyzed genes was correlated with the clinical-pathological features as well as with the survival of patients. In total, significant over-expression of all analyzed genes was observed in tumor tissues as well as in liver metastases except for LIN28A/B. Furthermore, it was shown that the deregulated levels of some of the analyzed genes significantly correlate with tumor stage, grade, location, size and lymph node positivity. Finally, high levels of DROSHA and TARBP2 were associated with shorter disease-free survival, while the over-expression of XPO5, TNRC6A and DDX17 was detected in tissues of patients with shorter overall survival and poor prognosis. Our data indicate that changed levels of miRNA biogenesis genes may contribute to origin as well as progression of CRC; thus, these molecules could serve as potential therapeutic targets.
ER -
VYCHYTILOVÁ, Petra, Alena SVOBODOVÁ KOVAŘÍKOVÁ, Tomáš GROLICH, Vladimír PROCHÁZKA, Kateřina SLABÁ, Táňa MACHÁČKOVÁ, Jana HALÁMKOVÁ, Marek SVOBODA, Zdeněk KALA, Igor KISS and Ondřej SLABÝ. MicroRNA Biogenesis Pathway Genes Are Deregulated in Colorectal Cancer. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2019, vol.~20, No~18, p.~1-13. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms20184460.
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