MicroRNA Biogenesis Pathway Genes Are Deregulated in Colorectal
Cancer

J 2019

MicroRNA Biogenesis Pathway Genes Are Deregulated in Colorectal Cancer

VYCHYTILOVÁ, Petra, Alena SVOBODOVÁ KOVAŘÍKOVÁ, Tomáš GROLICH, Vladimír PROCHÁZKA, Kateřina SLABÁ et. al.

Basic information

Original name

MicroRNA Biogenesis Pathway Genes Are Deregulated in Colorectal Cancer

Authors

VYCHYTILOVÁ, Petra (203 Czech Republic, belonging to the institution), Alena SVOBODOVÁ KOVAŘÍKOVÁ (203 Czech Republic, belonging to the institution), Tomáš GROLICH (203 Czech Republic, belonging to the institution), Vladimír PROCHÁZKA (203 Czech Republic, belonging to the institution), Kateřina SLABÁ (203 Czech Republic, belonging to the institution), Táňa MACHÁČKOVÁ (203 Czech Republic, belonging to the institution), Jana HALÁMKOVÁ (203 Czech Republic, belonging to the institution), Marek SVOBODA (203 Czech Republic, belonging to the institution), Zdeněk KALA (203 Czech Republic, belonging to the institution), Igor KISS (203 Czech Republic, belonging to the institution) and Ondřej SLABÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2019, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.556

RIV identification code

RIV/00216224:14740/19:00108569

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.3390/ijms20184460

UT WoS

000489100500139

Keywords in English

microRNA; biogenesis; colorectal cancer; disease-free survival; overall survival; RT-qPCR

Abstract

V originále

MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression. Each step of their production and maturation has to be strictly regulated, as any disruption of control mechanisms may lead to cancer. Thus, we have measured the expression of 19 genes involved in miRNAs biogenesis pathway in tumor tissues of 239 colorectal cancer (CRC) patients, 17 CRC patients with liver metastases and 239 adjacent tissues using real-time PCR. Subsequently, the expression of analyzed genes was correlated with the clinical-pathological features as well as with the survival of patients. In total, significant over-expression of all analyzed genes was observed in tumor tissues as well as in liver metastases except for LIN28A/B. Furthermore, it was shown that the deregulated levels of some of the analyzed genes significantly correlate with tumor stage, grade, location, size and lymph node positivity. Finally, high levels of DROSHA and TARBP2 were associated with shorter disease-free survival, while the over-expression of XPO5, TNRC6A and DDX17 was detected in tissues of patients with shorter overall survival and poor prognosis. Our data indicate that changed levels of miRNA biogenesis genes may contribute to origin as well as progression of CRC; thus, these molecules could serve as potential therapeutic targets.

Links

LM2015090, research and development project

Name: Český národní uzel Evropské sítě infrastruktur klinického výzkumu (Acronym: CZECRIN)

Investor: Ministry of Education, Youth and Sports of the CR

NV16-31765A, research and development project

Name: Využití tkáňových/cirkulujících mikroRNA pro predikci léčebné odpovědi a zpřesnění restagingu karcinomu rekta po neoadjuvantní léčbě

Citovat

VYCHYTILOVÁ, Petra, Alena SVOBODOVÁ KOVAŘÍKOVÁ, Tomáš GROLICH, Vladimír PROCHÁZKA, Kateřina SLABÁ, Táňa MACHÁČKOVÁ, Jana HALÁMKOVÁ, Marek SVOBODA, Zdeněk KALA, Igor KISS and Ondřej SLABÝ. MicroRNA Biogenesis Pathway Genes Are Deregulated in Colorectal Cancer. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, 2019, vol. 20, No 18, p. 1-13. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms20184460.

@article{1604997,
   author = {Vychytilová, Petra and Svobodová Kovaříková, Alena and Grolich, Tomáš and Procházka, Vladimír and Slabá, Kateřina and Macháčková, Táňa and Halámková, Jana and Svoboda, Marek and Kala, Zdeněk and Kiss, Igor and Slabý, Ondřej},
   article_location = {Basel},
   article_number = {18},
   doi = {http://dx.doi.org/10.3390/ijms20184460},
   keywords = {microRNA; biogenesis; colorectal cancer; disease-free survival; overall survival; RT-qPCR},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {MicroRNA Biogenesis Pathway Genes Are Deregulated in Colorectal Cancer},
   url = {https://www.mdpi.com/1422-0067/20/18/4460},
   volume = {20},
   year = {2019}
}

TY  - JOUR
ID  - 1604997
AU  - Vychytilová, Petra - Svobodová Kovaříková, Alena - Grolich, Tomáš - Procházka, Vladimír - Slabá, Kateřina - Macháčková, Táňa - Halámková, Jana - Svoboda, Marek - Kala, Zdeněk - Kiss, Igor - Slabý, Ondřej
PY  - 2019
TI  - MicroRNA Biogenesis Pathway Genes Are Deregulated in Colorectal Cancer
JF  - International Journal of Molecular Sciences
VL  - 20
IS  - 18
SP  - 1-13
EP  - 1-13
PB  - Multidisciplinary Digital Publishing Institute
SN  - 14220067
KW  - microRNA
KW  - biogenesis
KW  - colorectal cancer
KW  - disease-free survival
KW  - overall survival
KW  - RT-qPCR
UR  - https://www.mdpi.com/1422-0067/20/18/4460
L2  - https://www.mdpi.com/1422-0067/20/18/4460
N2  - MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression. Each step of their production and maturation has to be strictly regulated, as any disruption of control mechanisms may lead to cancer. Thus, we have measured the expression of 19 genes involved in miRNAs biogenesis pathway in tumor tissues of 239 colorectal cancer (CRC) patients, 17 CRC patients with liver metastases and 239 adjacent tissues using real-time PCR. Subsequently, the expression of analyzed genes was correlated with the clinical-pathological features as well as with the survival of patients. In total, significant over-expression of all analyzed genes was observed in tumor tissues as well as in liver metastases except for LIN28A/B. Furthermore, it was shown that the deregulated levels of some of the analyzed genes significantly correlate with tumor stage, grade, location, size and lymph node positivity. Finally, high levels of DROSHA and TARBP2 were associated with shorter disease-free survival, while the over-expression of XPO5, TNRC6A and DDX17 was detected in tissues of patients with shorter overall survival and poor prognosis. Our data indicate that changed levels of miRNA biogenesis genes may contribute to origin as well as progression of CRC; thus, these molecules could serve as potential therapeutic targets.
ER  -

VYCHYTILOVÁ, Petra, Alena SVOBODOVÁ KOVAŘÍKOVÁ, Tomáš GROLICH, Vladimír PROCHÁZKA, Kateřina SLABÁ, Táňa MACHÁČKOVÁ, Jana HALÁMKOVÁ, Marek SVOBODA, Zdeněk KALA, Igor KISS and Ondřej SLABÝ. MicroRNA Biogenesis Pathway Genes Are Deregulated in Colorectal Cancer. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2019, vol.~20, No~18, p.~1-13. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms20184460.

Detailed Information on Publication Record