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@article{1605463, author = {Noda, Angel A. and Rodriguez, Islay and Grillová, Linda and Bosshard, Philipp P. and Lienhard, Reto}, article_location = {LONDON}, article_number = {11}, doi = {http://dx.doi.org/10.1177/0956462419859764}, keywords = {Syphilis diagnosis; PCR; serology}, language = {eng}, issn = {0956-4624}, journal = {INTERNATIONAL JOURNAL OF STD & AIDS}, title = {Accuracy of PCR and serological testing for the diagnosis of primary syphilis: Both tests are necessary}, url = {http://dx.doi.org/10.1177/0956462419859764}, volume = {30}, year = {2019} }
TY - JOUR ID - 1605463 AU - Noda, Angel A. - Rodriguez, Islay - Grillová, Linda - Bosshard, Philipp P. - Lienhard, Reto PY - 2019 TI - Accuracy of PCR and serological testing for the diagnosis of primary syphilis: Both tests are necessary JF - INTERNATIONAL JOURNAL OF STD & AIDS VL - 30 IS - 11 SP - 1087-1094 EP - 1087-1094 PB - SAGE PUBLICATIONS LTD SN - 09564624 KW - Syphilis diagnosis KW - PCR KW - serology UR - http://dx.doi.org/10.1177/0956462419859764 L2 - http://dx.doi.org/10.1177/0956462419859764 N2 - Syphilis, caused by the spirochete Treponema pallidum subspecies pallidum, is a rising global public health concern and laboratory diagnostics remain challenging. Especially during early disease, rapid and accurate diagnosis is crucial to ensure patients and their contacts receive timely treatment to eradicate infection and prevent further transmission. In this prospective observational study, we evaluated the performance of polymerase chain reaction (PCR) and serological testing for the diagnosis of primary syphilis by evaluating anogenital swabs and sera from 178 Cuban patients presenting with ulcers. Three different PCR assays were evaluated targeting polA, tpp47 and 16S rDNA loci. Sera were evaluated with venereal disease research laboratory (VDRL) and T. pallidum hemagglutination (TPHA) assays. Assuming both methods were confirmatory, our data showed that PCR and serology did not correlate well (agreement = 52.3%, kappa 0.0512, 95% CI -0.0928-0.1951, p = 0.496). The sensitivities, specificities, positive and negative predictive values of the PCR assays were 76.1%, 100%, 100% and 57.9%, respectively, while the values for serology were 62.5%, 100%, 100% and 45.2%, respectively. The combination of PCR and serology can offer valuable information for the diagnosis of syphilis in patients presenting with anogenital ulceration avoiding further clinical complications and disease transmission. ER -
NODA, Angel A., Islay RODRIGUEZ, Linda GRILLOVÁ, Philipp P. BOSSHARD and Reto LIENHARD. Accuracy of PCR and serological testing for the diagnosis of primary syphilis: Both tests are necessary. \textit{INTERNATIONAL JOURNAL OF STD \&{}amp; AIDS}. LONDON: SAGE PUBLICATIONS LTD, 2019, vol.~30, No~11, p.~1087-1094. ISSN~0956-4624. Available from: https://dx.doi.org/10.1177/0956462419859764.
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