The Unique Mechanisms of Cellular Proliferation, Migration and
Apoptosis are Regulated through Oocyte Maturational
DevelopmentA Complete Transcriptomic and Histochemical Study

J 2019

The Unique Mechanisms of Cellular Proliferation, Migration and Apoptosis are Regulated through Oocyte Maturational DevelopmentA Complete Transcriptomic and Histochemical Study

CHERMULA, Blazej, Maciej BRAZERT, Michal JEŠETA, Katarzyna OZEGOWSKA, Patrycja SUJKA-KORDOWSKA et. al.

Basic information

Original name

The Unique Mechanisms of Cellular Proliferation, Migration and Apoptosis are Regulated through Oocyte Maturational DevelopmentA Complete Transcriptomic and Histochemical Study

Authors

CHERMULA, Blazej (616 Poland), Maciej BRAZERT (616 Poland), Michal JEŠETA (203 Czech Republic, belonging to the institution), Katarzyna OZEGOWSKA (616 Poland), Patrycja SUJKA-KORDOWSKA (616 Poland), Aneta KONWERSKA (616 Poland), Artur BRYJA (616 Poland), Wieslawa KRANC (616 Poland), Maurycy JANKOWSKI (616 Poland), Mariusz J. NAWROCKI (616 Poland), Ievgeniia KOCHEROVA (616 Poland), Piotr CELICHOWSKI (616 Poland), Blanka BOROWIEC (616 Poland), Malgorzata POPIS (616 Poland), Joanna BUDNA-TUKAN (616 Poland), Pawel ANTOSIK (616 Poland), Dorota BUKOWSKA (616 Poland), Klaus P. BRUSSOW (616 Poland), Leszek PAWELCZYK (616 Poland), Malgorzata BRUSKA (616 Poland), Maciej ZABEL (616 Poland), Michal NOWICKI (616 Poland) and Bartosz KEMPISTY (616 Poland)

Edition

International Journal of Molecular Sciences, Basel, Switzerland, MDPI AG, 2019, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.556

RIV identification code

RIV/00216224:14110/19:00112512

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/ijms20010084

UT WoS

000459747700084

Keywords in English

pig; oocytes; microarray; cellular competence

Abstract

V originále

The growth and development of oocyte affect the functional activities of the surrounding somatic cells. These cells are regulated by various types of hormones, proteins, metabolites, and regulatory molecules through gap communication, ultimately leading to the development and maturation of oocytes. The close association between somatic cells and oocytes, which together form the cumulus-oocyte complexes (COCs), and their bi-directional communication are crucial for the acquisition of developmental competences by the oocyte. In this study, oocytes were extracted from the ovaries obtained from crossbred landrace gilts and subjected to in vitro maturation. RNA isolated from those oocytes was used for the subsequent microarray analysis. The data obtained shows, for the first time, variable levels of gene expression (fold changes higher than |2| and adjusted p-value < 0.05) belonging to four ontological groups: regulation of cell proliferation (GO:0042127), regulation of cell migration (GO:0030334), and regulation of programmed cell death (GO:0043067) that can be used together as proliferation, migration or apoptosis markers. We have identified several genes of porcine oocytes (ID2, VEGFA, BTG2, ESR1, CCND2, EDNRA, ANGPTL4, TGFBR3, GJA1, LAMA2, KIT, TPM1, VCP, GRID2, MEF2C, RPS3A, PLD1, BTG3, CD47, MITF), whose expression after in vitro maturation (IVM) is downregulated with different degrees. Our results may be helpful in further elucidating the molecular basis and functional significance of a number of gene markers associated with the processes of migration, proliferation and angiogenesis occurring in COCs.

Citovat

CHERMULA, Blazej, Maciej BRAZERT, Michal JEŠETA, Katarzyna OZEGOWSKA, Patrycja SUJKA-KORDOWSKA, Aneta KONWERSKA, Artur BRYJA, Wieslawa KRANC, Maurycy JANKOWSKI, Mariusz J. NAWROCKI, Ievgeniia KOCHEROVA, Piotr CELICHOWSKI, Blanka BOROWIEC, Malgorzata POPIS, Joanna BUDNA-TUKAN, Pawel ANTOSIK, Dorota BUKOWSKA, Klaus P. BRUSSOW, Leszek PAWELCZYK, Malgorzata BRUSKA, Maciej ZABEL, Michal NOWICKI and Bartosz KEMPISTY. The Unique Mechanisms of Cellular Proliferation, Migration and Apoptosis are Regulated through Oocyte Maturational DevelopmentA Complete Transcriptomic and Histochemical Study. International Journal of Molecular Sciences. Basel, Switzerland: MDPI AG, 2019, vol. 20, No 1, p. 1-21. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms20010084.

@article{1605582,
   author = {Chermula, Blazej and Brazert, Maciej and Ješeta, Michal and Ozegowska, Katarzyna and SujkaandKordowska, Patrycja and Konwerska, Aneta and Bryja, Artur and Kranc, Wieslawa and Jankowski, Maurycy and Nawrocki, Mariusz J. and Kocherova, Ievgeniia and Celichowski, Piotr and Borowiec, Blanka and Popis, Malgorzata and BudnaandTukan, Joanna and Antosik, Pawel and Bukowska, Dorota and Brussow, Klaus P. and Pawelczyk, Leszek and Bruska, Malgorzata and Zabel, Maciej and Nowicki, Michal and Kempisty, Bartosz},
   article_location = {Basel, Switzerland},
   article_number = {1},
   doi = {http://dx.doi.org/10.3390/ijms20010084},
   keywords = {pig; oocytes; microarray; cellular competence},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {The Unique Mechanisms of Cellular Proliferation, Migration and Apoptosis are Regulated through Oocyte Maturational DevelopmentA Complete Transcriptomic and Histochemical Study},
   url = {http://dx.doi.org/10.3390/ijms20010084},
   volume = {20},
   year = {2019}
}

TY  - JOUR
ID  - 1605582
AU  - Chermula, Blazej - Brazert, Maciej - Ješeta, Michal - Ozegowska, Katarzyna - Sujka-Kordowska, Patrycja - Konwerska, Aneta - Bryja, Artur - Kranc, Wieslawa - Jankowski, Maurycy - Nawrocki, Mariusz J. - Kocherova, Ievgeniia - Celichowski, Piotr - Borowiec, Blanka - Popis, Malgorzata - Budna-Tukan, Joanna - Antosik, Pawel - Bukowska, Dorota - Brussow, Klaus P. - Pawelczyk, Leszek - Bruska, Malgorzata - Zabel, Maciej - Nowicki, Michal - Kempisty, Bartosz
PY  - 2019
TI  - The Unique Mechanisms of Cellular Proliferation, Migration and Apoptosis are Regulated through Oocyte Maturational DevelopmentA Complete Transcriptomic and Histochemical Study
JF  - International Journal of Molecular Sciences
VL  - 20
IS  - 1
SP  - 1-21
EP  - 1-21
PB  - MDPI AG
SN  - 14220067
KW  - pig
KW  - oocytes
KW  - microarray
KW  - cellular competence
UR  - http://dx.doi.org/10.3390/ijms20010084
L2  - http://dx.doi.org/10.3390/ijms20010084
N2  - The growth and development of oocyte affect the functional activities of the surrounding somatic cells. These cells are regulated by various types of hormones, proteins, metabolites, and regulatory molecules through gap communication, ultimately leading to the development and maturation of oocytes. The close association between somatic cells and oocytes, which together form the cumulus-oocyte complexes (COCs), and their bi-directional communication are crucial for the acquisition of developmental competences by the oocyte. In this study, oocytes were extracted from the ovaries obtained from crossbred landrace gilts and subjected to in vitro maturation. RNA isolated from those oocytes was used for the subsequent microarray analysis. The data obtained shows, for the first time, variable levels of gene expression (fold changes higher than |2| and adjusted p-value < 0.05) belonging to four ontological groups: regulation of cell proliferation (GO:0042127), regulation of cell migration (GO:0030334), and regulation of programmed cell death (GO:0043067) that can be used together as proliferation, migration or apoptosis markers. We have identified several genes of porcine oocytes (ID2, VEGFA, BTG2, ESR1, CCND2, EDNRA, ANGPTL4, TGFBR3, GJA1, LAMA2, KIT, TPM1, VCP, GRID2, MEF2C, RPS3A, PLD1, BTG3, CD47, MITF), whose expression after in vitro maturation (IVM) is downregulated with different degrees. Our results may be helpful in further elucidating the molecular basis and functional significance of a number of gene markers associated with the processes of migration, proliferation and angiogenesis occurring in COCs.
ER  -

CHERMULA, Blazej, Maciej BRAZERT, Michal JEŠETA, Katarzyna OZEGOWSKA, Patrycja SUJKA-KORDOWSKA, Aneta KONWERSKA, Artur BRYJA, Wieslawa KRANC, Maurycy JANKOWSKI, Mariusz J. NAWROCKI, Ievgeniia KOCHEROVA, Piotr CELICHOWSKI, Blanka BOROWIEC, Malgorzata POPIS, Joanna BUDNA-TUKAN, Pawel ANTOSIK, Dorota BUKOWSKA, Klaus P. BRUSSOW, Leszek PAWELCZYK, Malgorzata BRUSKA, Maciej ZABEL, Michal NOWICKI and Bartosz KEMPISTY. The Unique Mechanisms of Cellular Proliferation, Migration and Apoptosis are Regulated through Oocyte Maturational DevelopmentA Complete Transcriptomic and Histochemical Study. \textit{International Journal of Molecular Sciences}. Basel, Switzerland: MDPI AG, 2019, vol.~20, No~1, p.~1-21. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms20010084.

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