The role of miR-409-3p in regulation of HPV16/18-E6 mRNA in
human cervical high-grade squamous intraepithelial lesions

J 2019

The role of miR-409-3p in regulation of HPV16/18-E6 mRNA in human cervical high-grade squamous intraepithelial lesions

SOMMEROVA, Lucia, Milan ANTON, Pavla BOUCHALOVÁ, Hedvika JASICKOVA, Vladimír RAK et. al.

Basic information

Original name

The role of miR-409-3p in regulation of HPV16/18-E6 mRNA in human cervical high-grade squamous intraepithelial lesions

Authors

SOMMEROVA, Lucia (203 Czech Republic), Milan ANTON (203 Czech Republic, belonging to the institution), Pavla BOUCHALOVÁ (203 Czech Republic), Hedvika JASICKOVA (203 Czech Republic), Vladimír RAK (203 Czech Republic), Eva JANDÁKOVÁ (203 Czech Republic, belonging to the institution), Iveta SELINGEROVÁ (203 Czech Republic), Martin BARTOSIK (203 Czech Republic), Bořivoj VOJTĚŠEK (203 Czech Republic) and Roman HRSTKA (203 Czech Republic, guarantor)

Edition

ANTIVIRAL RESEARCH, AMSTERDAM, ELSEVIER SCIENCE BV, 2019, 0166-3542

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.101

RIV identification code

RIV/00216224:14110/19:00108061

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.antiviral.2019.01.019

UT WoS

000459843400019

Keywords in English

Cervical cancer; HPV; miRNA; LSIL; HSIL; hsa-miR-409-3p; E6 mRNA

Abstract

V originále

Cervical cancer is one of the most common malignancies in women. MicroRNAs (miRNAs) are involved in a variety of fundamental cellular processes, including carcinogenesis. The potential utilization of aberrantly expressed miRNAs as novel biomarkers in cervical cancer diagnostics is growing. We investigated miRNA expression profiles during the progression of dysplasia in cervical epithelium to identify aberrantly expressed miRNAs. High-throughput miRNA profiling of high-grade precancerous lesions identified 79 miRNAs showing significant difference in expression values compared to normal cervical epithelium. Ten selected miRNAs were subsequently measured in an independent group of samples to validate them as promising biomarkers of cervical carcinogenesis. MicroRNAs miR-10b-5p, miR-34c-5p, miR-409-3p and miR-411-5p were confirmed as down regulated, while miR-10a-5p, miR-132-3p, miR-141-5p were significantly upregulated in dysplastic cervical tissues. Further investigation revealed an inverse correlation of miR-409-3p with E6 mRNA levels in pre-cancerous cervical lesions. Subsequent in vitro analyses showed a direct involvement of this miRNA in the regulation of E6 oncogene levels, thus confirming a potential tumor suppressor function of miR-409-3p in cervical malignancies. Hence, miR-409-3p may represent a useful early marker and a potential therapeutic target for cervical cancer.

Links

GBP206/12/G151, research and development project

Name: Centrum nových přístupů k bioanalýze a molekulární diagnostice

Citovat

SOMMEROVA, Lucia, Milan ANTON, Pavla BOUCHALOVÁ, Hedvika JASICKOVA, Vladimír RAK, Eva JANDÁKOVÁ, Iveta SELINGEROVÁ, Martin BARTOSIK, Bořivoj VOJTĚŠEK and Roman HRSTKA. The role of miR-409-3p in regulation of HPV16/18-E6 mRNA in human cervical high-grade squamous intraepithelial lesions. ANTIVIRAL RESEARCH. AMSTERDAM: ELSEVIER SCIENCE BV, 2019, vol. 163, MAR 2019, p. 185-192. ISSN 0166-3542. Available from: https://dx.doi.org/10.1016/j.antiviral.2019.01.019.

@article{1605602,
   author = {Sommerova, Lucia and Anton, Milan and Bouchalová, Pavla and Jasickova, Hedvika and Rak, Vladimír and Jandáková, Eva and Selingerová, Iveta and Bartosik, Martin and Vojtěšek, Bořivoj and Hrstka, Roman},
   article_location = {AMSTERDAM},
   article_number = {MAR 2019},
   doi = {http://dx.doi.org/10.1016/j.antiviral.2019.01.019},
   keywords = {Cervical cancer; HPV; miRNA; LSIL; HSIL; hsa-miR-409-3p; E6 mRNA},
   language = {eng},
   issn = {0166-3542},
   journal = {ANTIVIRAL RESEARCH},
   title = {The role of miR-409-3p in regulation of HPV16/18-E6 mRNA in human cervical high-grade squamous intraepithelial lesions},
   url = {http://dx.doi.org/10.1016/j.antiviral.2019.01.019},
   volume = {163},
   year = {2019}
}

TY  - JOUR
ID  - 1605602
AU  - Sommerova, Lucia - Anton, Milan - Bouchalová, Pavla - Jasickova, Hedvika - Rak, Vladimír - Jandáková, Eva - Selingerová, Iveta - Bartosik, Martin - Vojtěšek, Bořivoj - Hrstka, Roman
PY  - 2019
TI  - The role of miR-409-3p in regulation of HPV16/18-E6 mRNA in human cervical high-grade squamous intraepithelial lesions
JF  - ANTIVIRAL RESEARCH
VL  - 163
IS  - MAR 2019
SP  - 185-192
EP  - 185-192
PB  - ELSEVIER SCIENCE BV
SN  - 01663542
KW  - Cervical cancer
KW  - HPV
KW  - miRNA
KW  - LSIL
KW  - HSIL
KW  - hsa-miR-409-3p
KW  - E6 mRNA
UR  - http://dx.doi.org/10.1016/j.antiviral.2019.01.019
L2  - http://dx.doi.org/10.1016/j.antiviral.2019.01.019
N2  - Cervical cancer is one of the most common malignancies in women. MicroRNAs (miRNAs) are involved in a variety of fundamental cellular processes, including carcinogenesis. The potential utilization of aberrantly expressed miRNAs as novel biomarkers in cervical cancer diagnostics is growing. We investigated miRNA expression profiles during the progression of dysplasia in cervical epithelium to identify aberrantly expressed miRNAs. High-throughput miRNA profiling of high-grade precancerous lesions identified 79 miRNAs showing significant difference in expression values compared to normal cervical epithelium. Ten selected miRNAs were subsequently measured in an independent group of samples to validate them as promising biomarkers of cervical carcinogenesis. MicroRNAs miR-10b-5p, miR-34c-5p, miR-409-3p and miR-411-5p were confirmed as down regulated, while miR-10a-5p, miR-132-3p, miR-141-5p were significantly upregulated in dysplastic cervical tissues. Further investigation revealed an inverse correlation of miR-409-3p with E6 mRNA levels in pre-cancerous cervical lesions. Subsequent in vitro analyses showed a direct involvement of this miRNA in the regulation of E6 oncogene levels, thus confirming a potential tumor suppressor function of miR-409-3p in cervical malignancies. Hence, miR-409-3p may represent a useful early marker and a potential therapeutic target for cervical cancer.
ER  -

SOMMEROVA, Lucia, Milan ANTON, Pavla BOUCHALOVÁ, Hedvika JASICKOVA, Vladimír RAK, Eva JANDÁKOVÁ, Iveta SELINGEROVÁ, Martin BARTOSIK, Bořivoj VOJTĚŠEK and Roman HRSTKA. The role of miR-409-3p in regulation of HPV16/18-E6 mRNA in human cervical high-grade squamous intraepithelial lesions. \textit{ANTIVIRAL RESEARCH}. AMSTERDAM: ELSEVIER SCIENCE BV, 2019, vol.~163, MAR 2019, p.~185-192. ISSN~0166-3542. Available from: https://dx.doi.org/10.1016/j.antiviral.2019.01.019.

Detailed Information on Publication Record