Proteomics Identification and Validation of Desmocollin-1 and Catechol-O-Methyltransferase as Proteins Associated with Breast Cancer Cell Migration and Metastasis

FAKTOR, Jakub, Lucia KNOPFOVÁ, Petr LAPČÍK, Lucia JANÁČOVÁ, Vendula PÁRALOVÁ, Pavla BOUCHALOVÁ, Petr MÜLLER, Petr BENEŠ and Pavel BOUCHAL. Proteomics Identification and Validation of Desmocollin-1 and Catechol-O-Methyltransferase as Proteins Associated with Breast Cancer Cell Migration and Metastasis. Proteomics. HOBOKEN, USA: Wiley, 2019, vol. 19, 21-22, p. 1-11. ISSN 1615-9853. Available from: https://dx.doi.org/10.1002/pmic.201900073.

Basic information

• Original name: Proteomics Identification and Validation of Desmocollin-1 and Catechol-O-Methyltransferase as Proteins Associated with Breast Cancer Cell Migration and Metastasis
• Authors: FAKTOR, Jakub (703 Slovakia), Lucia KNOPFOVÁ (203 Czech Republic, belonging to the institution), Petr LAPČÍK (203 Czech Republic, belonging to the institution), Lucia JANÁČOVÁ (703 Slovakia, belonging to the institution), Vendula PÁRALOVÁ (203 Czech Republic, belonging to the institution), Pavla BOUCHALOVÁ (203 Czech Republic, belonging to the institution), Petr MÜLLER (203 Czech Republic), Petr BENEŠ (203 Czech Republic, belonging to the institution) and Pavel BOUCHAL (203 Czech Republic, belonging to the institution).
• Edition: Proteomics, HOBOKEN, USA, Wiley, 2019, 1615-9853.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10609 Biochemical research methods
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 3.254
• RIV identification code: RIV/00216224:14310/19:00108076
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1002/pmic.201900073
• UT WoS: 000492737800001
• Keywords in English: breast cancer; mass spectrometry; metastasis; sequential window acquisition of all theoretical; transmembrane proteins
• Tags: CF PROT, rivok
• Tags: International impact, Reviewed

Abstract

Biological treatment of many cancers currently targets membrane bound receptors located on a cell surface. To identify novel membrane proteins associated with migration and metastasis of breast cancer cells, a more migrating subpopulation of MDA-MB-231 breast cancer cell line is selected and characterized. A high-resolution quantitative mass spectrometry with SILAC labeling is applied to analyze their surfaceome and it is compared with that of parental MDA-MB-231 cells. Among 824 identified proteins (FDR < 0.01), 128 differentially abundant cell surface proteins with at least one transmembrane domain are found. Of these, i) desmocollin-1 (DSC1) is validated as a protein connected with lymph node status of luminal A breast cancer, tumor grade, and Her-2 status by immunohistochemistry in the set of 96 primary breast tumors, and ii) catechol-O-methyltransferase is successfully verified as a protein associated with lymph node metastasis of triple negative breast cancer as well as with tumor grade by targeted data extraction from the SWATH-MS data of the same set of tissues. The findings indicate importance of both proteins for breast cancer development and metastasis and highlight the potential of biomarker validation strategy via targeted data extraction from SWATH-MS datasets.

Links

• GA17-05957S, research and development project: Name: Evaluace nových potenciálních cílů a inhibitorů pro blokování vývoje metastáz u luminálních A nádorů prsu

Investor: Czech Science Foundation

• LM2015043, research and development project: Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

• MUNI/A/1575/2018, interní kód MU: Name: Podpora biochemického výzkumu v roce 2019

Investor: Masaryk University, Category A