J 2019

Proteomics Identification and Validation of Desmocollin-1 and Catechol-O-Methyltransferase as Proteins Associated with Breast Cancer Cell Migration and Metastasis

FAKTOR, Jakub, Lucia KNOPFOVÁ, Petr LAPČÍK, Lucia JANÁČOVÁ, Vendula PÁRALOVÁ et. al.

Basic information

Original name

Proteomics Identification and Validation of Desmocollin-1 and Catechol-O-Methyltransferase as Proteins Associated with Breast Cancer Cell Migration and Metastasis

Authors

FAKTOR, Jakub (703 Slovakia), Lucia KNOPFOVÁ (203 Czech Republic, belonging to the institution), Petr LAPČÍK (203 Czech Republic, belonging to the institution), Lucia JANÁČOVÁ (703 Slovakia, belonging to the institution), Vendula PÁRALOVÁ (203 Czech Republic, belonging to the institution), Pavla BOUCHALOVÁ (203 Czech Republic, belonging to the institution), Petr MÜLLER (203 Czech Republic), Petr BENEŠ (203 Czech Republic, belonging to the institution) and Pavel BOUCHAL (203 Czech Republic, belonging to the institution)

Edition

Proteomics, HOBOKEN, USA, Wiley, 2019, 1615-9853

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10609 Biochemical research methods

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 3.254

RIV identification code

RIV/00216224:14310/19:00108076

Organization unit

Faculty of Science

DOI

UT WoS

000492737800001

Keywords in English

breast cancer; mass spectrometry; metastasis; sequential window acquisition of all theoretical; transmembrane proteins

Tags

Tags

International impact, Reviewed
Změněno: 7/2/2023 09:04, doc. Mgr. Pavel Bouchal, Ph.D.

Abstract

V originále

Biological treatment of many cancers currently targets membrane bound receptors located on a cell surface. To identify novel membrane proteins associated with migration and metastasis of breast cancer cells, a more migrating subpopulation of MDA-MB-231 breast cancer cell line is selected and characterized. A high-resolution quantitative mass spectrometry with SILAC labeling is applied to analyze their surfaceome and it is compared with that of parental MDA-MB-231 cells. Among 824 identified proteins (FDR < 0.01), 128 differentially abundant cell surface proteins with at least one transmembrane domain are found. Of these, i) desmocollin-1 (DSC1) is validated as a protein connected with lymph node status of luminal A breast cancer, tumor grade, and Her-2 status by immunohistochemistry in the set of 96 primary breast tumors, and ii) catechol-O-methyltransferase is successfully verified as a protein associated with lymph node metastasis of triple negative breast cancer as well as with tumor grade by targeted data extraction from the SWATH-MS data of the same set of tissues. The findings indicate importance of both proteins for breast cancer development and metastasis and highlight the potential of biomarker validation strategy via targeted data extraction from SWATH-MS datasets.

Links

GA17-05957S, research and development project
Name: Evaluace nových potenciálních cílů a inhibitorů pro blokování vývoje metastáz u luminálních A nádorů prsu
Investor: Czech Science Foundation
LM2015043, research and development project
Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)
Investor: Ministry of Education, Youth and Sports of the CR
MUNI/A/1575/2018, interní kód MU
Name: Podpora biochemického výzkumu v roce 2019
Investor: Masaryk University, Category A