Emergence of Rare Bovine-Human Reassortant DS-1-Like Rotavirus
A Strains with G8P[8] Genotype in Human Patients in the Czech
Republic

J 2019

Emergence of Rare Bovine-Human Reassortant DS-1-Like Rotavirus A Strains with G8P[8] Genotype in Human Patients in the Czech Republic

MOUTELÍKOVÁ, Romana, Pavel SAUER, Monika DVOŘÁKOVÁ HEROLDOVÁ, Veronika HOLÁ, Jana PRODĚLALOVÁ et. al.

Basic information

Original name

Emergence of Rare Bovine-Human Reassortant DS-1-Like Rotavirus A Strains with G8P[8] Genotype in Human Patients in the Czech Republic

Authors

MOUTELÍKOVÁ, Romana (203 Czech Republic, guarantor), Pavel SAUER (203 Czech Republic), Monika DVOŘÁKOVÁ HEROLDOVÁ (203 Czech Republic, belonging to the institution), Veronika HOLÁ (203 Czech Republic, belonging to the institution) and Jana PRODĚLALOVÁ (203 Czech Republic)

Edition

VIRUSES-BASEL, BASEL, MDPI AG, 2019, 1999-4915

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10607 Virology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.816

RIV identification code

RIV/00216224:14110/19:00108575

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/v11111015

UT WoS

000502292300043

Keywords in English

rotavirus A; G8; gastroenteritis; bovine-human reassortants; Central Europe

Abstract

V originále

Group A Rotaviruses (RVA) are the leading cause of acute gastroenteritis in children and a major cause of childhood mortality in low-income countries. RVAs are mostly host-specific, but interspecies transmission and reassortment between human and animal RVAs significantly contribute to their genetic diversity. We investigated the VP7 and VP4 genotypes of RVA isolated from 225 stool specimens collected from Czech patients with gastroenteritis during 2016-2019. The most abundant genotypes were G1P[8] (42.7%), G3P[8] (11.1%), G9P[8] (9.8%), G2P[4] (4.4%), G4P[8] (1.3%), G12P[8] (1.3%), and, surprisingly, G8P[8] (9.3%). Sequence analysis of G8P[8] strains revealed the highest nucleotide similarity of all Czech G8 sequences to the G8P[8] rotavirus strains that were isolated in Vietnam in 2014/2015. The whole-genome backbone of the Czech G8 strains was determined with the use of next-generation sequencing as DS-1-like. Phylogenetic analysis of all segments clustered the Czech isolates with RVA strains that were formerly described in Southeast Asia, which had emerged following genetic reassortment between bovine and human RVAs. This is the first time that bovine-human DS-1-like G8P[8] strains were detected at a high rate in human patients in Central Europe. Whether the emergence of this unusual genotype reflects the establishment of a new RVA strain in the population requires the continuous monitoring of rotavirus epidemiology.

Links

NV16-29937A, research and development project

Name: Komplexní analýza humánních rotavirových infekcí v České republice včetně atypických a emergentních kmenů směřující k vývoji nových detekčních metod

Citovat

MOUTELÍKOVÁ, Romana, Pavel SAUER, Monika DVOŘÁKOVÁ HEROLDOVÁ, Veronika HOLÁ and Jana PRODĚLALOVÁ. Emergence of Rare Bovine-Human Reassortant DS-1-Like Rotavirus A Strains with G8P[8] Genotype in Human Patients in the Czech Republic. VIRUSES-BASEL. BASEL: MDPI AG, 2019, vol. 11, No 11, p. 1-14. ISSN 1999-4915. Available from: https://dx.doi.org/10.3390/v11111015.

@article{1607099,
   author = {Moutelíková, Romana and Sauer, Pavel and Dvořáková Heroldová, Monika and Holá, Veronika and Prodělalová, Jana},
   article_location = {BASEL},
   article_number = {11},
   doi = {http://dx.doi.org/10.3390/v11111015},
   keywords = {rotavirus A; G8; gastroenteritis; bovine-human reassortants; Central Europe},
   language = {eng},
   issn = {1999-4915},
   journal = {VIRUSES-BASEL},
   title = {Emergence of Rare Bovine-Human Reassortant DS-1-Like Rotavirus A Strains with G8P[8] Genotype in Human Patients in the Czech Republic},
   url = {http://dx.doi.org/10.3390/v11111015},
   volume = {11},
   year = {2019}
}

TY  - JOUR
ID  - 1607099
AU  - Moutelíková, Romana - Sauer, Pavel - Dvořáková Heroldová, Monika - Holá, Veronika - Prodělalová, Jana
PY  - 2019
TI  - Emergence of Rare Bovine-Human Reassortant DS-1-Like Rotavirus A Strains with G8P[8] Genotype in Human Patients in the Czech Republic
JF  - VIRUSES-BASEL
VL  - 11
IS  - 11
SP  - 1-14
EP  - 1-14
PB  - MDPI AG
SN  - 19994915
KW  - rotavirus A
KW  - G8
KW  - gastroenteritis
KW  - bovine-human reassortants
KW  - Central Europe
UR  - http://dx.doi.org/10.3390/v11111015
L2  - http://dx.doi.org/10.3390/v11111015
N2  - Group A Rotaviruses (RVA) are the leading cause of acute gastroenteritis in children and a major cause of childhood mortality in low-income countries. RVAs are mostly host-specific, but interspecies transmission and reassortment between human and animal RVAs significantly contribute to their genetic diversity. We investigated the VP7 and VP4 genotypes of RVA isolated from 225 stool specimens collected from Czech patients with gastroenteritis during 2016-2019. The most abundant genotypes were G1P[8] (42.7%), G3P[8] (11.1%), G9P[8] (9.8%), G2P[4] (4.4%), G4P[8] (1.3%), G12P[8] (1.3%), and, surprisingly, G8P[8] (9.3%). Sequence analysis of G8P[8] strains revealed the highest nucleotide similarity of all Czech G8 sequences to the G8P[8] rotavirus strains that were isolated in Vietnam in 2014/2015. The whole-genome backbone of the Czech G8 strains was determined with the use of next-generation sequencing as DS-1-like. Phylogenetic analysis of all segments clustered the Czech isolates with RVA strains that were formerly described in Southeast Asia, which had emerged following genetic reassortment between bovine and human RVAs. This is the first time that bovine-human DS-1-like G8P[8] strains were detected at a high rate in human patients in Central Europe. Whether the emergence of this unusual genotype reflects the establishment of a new RVA strain in the population requires the continuous monitoring of rotavirus epidemiology.
ER  -

MOUTELÍKOVÁ, Romana, Pavel SAUER, Monika DVOŘÁKOVÁ HEROLDOVÁ, Veronika HOLÁ and Jana PRODĚLALOVÁ. Emergence of Rare Bovine-Human Reassortant DS-1-Like Rotavirus A Strains with G8P[8] Genotype in Human Patients in the Czech Republic. \textit{VIRUSES-BASEL}. BASEL: MDPI AG, 2019, vol.~11, No~11, p.~1-14. ISSN~1999-4915. Available from: https://dx.doi.org/10.3390/v11111015.

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