MAZUR, Ewa, Michelle GALLEI, Maciek ADAMOWSKI, Huibin HAN, Helene ROBERT BOISIVON and Jiří FRIML. Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization and vascular tissue formation in Arabidopsis. Plant Science. Ireland: Elsevier, 2020, vol. 293, APR, p. 110414-110424. ISSN 0168-9452. doi:10.1016/j.plantsci.2020.110414.
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Basic information
Original name Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization and vascular tissue formation in Arabidopsis
Authors MAZUR, Ewa (616 Poland, belonging to the institution), Michelle GALLEI (40 Austria), Maciek ADAMOWSKI (616 Poland), Huibin HAN (156 China), Helene ROBERT BOISIVON (250 France, guarantor, belonging to the institution) and Jiří FRIML (203 Czech Republic).
Edition Plant Science, Ireland, Elsevier, 2020, 0168-9452.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10605 Developmental biology
Country of publisher Ireland
Confidentiality degree is not subject to a state or trade secret
WWW Full text
Impact factor Impact factor: 4.729
RIV identification code RIV/00216224:14740/20:00114034
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1016/j.plantsci.2020.110414
UT WoS 000520609800009
Keywords in English auxin; auxin canalization; cell polarity; PIN1; clathrin-mediated trafficking; endocytosis; endocytic trafficking
Tags CF CELLIM, CF PLANT, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Pavla Foltynová, Ph.D., učo 106624. Changed: 29/4/2021 12:02.
Abstract
The flexible development of plants is characterized by a high capacity for post-embryonic organ formation and tissue regeneration, processes, which require tightly regulated intercellular communication and coordinated tissue (re-)polarization. The phytohormone auxin, the main driver for these processes, is able to establish polarized auxin transport channels, which are characterized by the expression and polar, subcellular localization of the PIN1 auxin transport proteins. These channels are demarcating the position of future vascular strands necessary for organ formation and tissue regeneration. Major progress has been made in the last years to understand how PINs can change their polarity in different contexts and thus guide auxin flow through the plant. However, it still remains elusive how auxin mediates the establishment of auxin conducting channels and the formation of vascular tissue and which cellular processes are involved. By the means of sophisticated regeneration experiments combined with local auxin applications in Arabidopsis thaliana inflorescence stems we show that (i) PIN subcellular dynamics, (ii) PIN internalization by clathrin-mediated trafficking and (iii) an intact actin cytoskeleton required for post-endocytic trafficking are indispensable for auxin channel formation, de novo vascular formation and vascular regeneration after wounding. These observations provide novel insights into cellular mechanism of coordinated tissue polarization during auxin canalization.
Links
GA13-40637S, research and development projectName: Genetické studie k identifikaci molekulárních mechanizmů buněčné polarity a auxinového transportu v rostlinách
Investor: Czech Science Foundation
GA18-26981S, research and development projectName: Genetické studie k objasnění molekulárního mechanizmu účinku strigolaktonů v kořeni
Investor: Czech Science Foundation
LM2015062, research and development projectName: Národní infrastruktura pro biologické a medicínské zobrazování
Investor: Ministry of Education, Youth and Sports of the CR
LQ1601, research and development projectName: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
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